Influence of culture conditions on the biosynthesis of Schwanniomyces castellii phytase

Lambrechts, Christel; Boze, Hélène; Segueilha, Laurent; Moulin, G.; Galzy, P.

doi:10.1007/bf00128284

Cited by 28 publications

(12 citation statements)

References 7 publications

(10 reference statements)

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“…Ebune et al (1995) reported that in a solid-state fermentation with A. ficuum, supplementation of 1 mg phosphate to 100 g medium produced the highest amount of phytase, but the production was inhibited when 10 mg and 50 mg of phosphate were added to the medium. Phytase synthesis by Schwanniomyces castellii was strongly inhibited when 1AE0 mmol l )1 phosphate (equivalent to about 132 mg l )1 ) or more was added (Lambrechts et al 1993). In the case of bacteria, it was reported that the optimal phosphate level for phytase production in Lact.…”

Section: Discussionmentioning

confidence: 99%

“…They also observed that glucose concentration in the medium had an effect on enzyme production. In continuous culture, Lambrechts et al . (1993) found that the level of phytase production by Schwanniomyces castellii increased with the increase of pH (when the pH of medium was over 6·0) and dilution rate, but decreased when the phytic acid or phosphate content increased.…”

Section: Introductionmentioning

confidence: 99%

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Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Lan

Abdullah²,

Jalaludin

et al. 2002

Journal of Applied Microbiology

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Aims: The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. Method and Results: By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by sodium phytate. Inclusion of glucose into rice bran-soybean milk (RB-SM) medium inhibited the phytase production. Supplementation of 0AE05-0AE5% of phosphate to RB-SM medium neither inhibited nor stimulated the phytase production. The optimum pH and temperature for the phytase production of M. jalaludinii were 7AE02 and 39°C, respectively. Surfactants such as Tween-80 (0AE5% and 1AE0%) and Triton X-100 (1AE0%) did not affect the phytase production. Conclusions, Significance and Impact of the Study: The study has provided important data on the culture conditions affecting phytase production of M. jalaludinii, which will facilitate future research on the optimization of fermentation processes for production of high phytase activity by M. jalaludinii.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Lan

Abdullah²,

Jalaludin

et al. 2002

Journal of Applied Microbiology

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“…Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been puriWed and characterized from various molds [37,38,49,50,54], bacteria [11,21,35,47] and yeasts [25,26,53]. Various researchers have shown the eVect of range of parameters including the fermentation technique adopted, culture conditions, inoculum age and size, and media nutrients on the production of phytase [9,12,25,52]. The fermentation conditions (pH 5.3, 30°C…”

Section: Introductionmentioning

confidence: 99%

Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Gunashree

Venkateswaran

2008

J Ind Microbiol Biotechnol

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The present article deals with the studies on the effect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF. Sodium dihydrogen phosphate (NaH(2)PO(4)) favored 34% higher enzyme yield than the control, which was followed by 19% higher activity in potassium dihydrogen phosphate (KH(2)PO(4)) in SSF at 0.015% w/v. The addition of Tween-20 in SmF showed a maximum yield of 12.6 U/mL while, SDS suppressed the growth of the fungus. None of the surfactants favored the enzyme yield in SSF. Calcium chloride (CaCl(2)) was extensively efficient in stimulating more than 55% higher phytase production in SmF at 0.01% v/v. In SSF, none of the metal salts stimulated phytase production.

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“…the initial pH and the temperature used for cultivation of the microorganisms. Both biomass and enzyme production respond to all these parameters, but temperature and pH for maximal growth and production of phytate-degrading enzymes were shown to be different in some moulds and yeast (Lambrechts et al, 1993;Kim et al, 1999;Sano et al, 1999;Mandviwala and Khire, 2000;Andlid et al, 2004), indicating a biomass-independent effect of temperature and pH on the synthesis of phytate-degrading enzymes in these microorganisms. No comparable studies have been performed so far in bacteria.…”

Section: Regulation Of Phytate-degrading Enzyme Formation In Microorgmentioning

confidence: 99%

Phytate-degrading enzymes: regulation of synthesis in microorganisms and plants.

Greiner

2006

Inositol Phosphates: Linking Agriculture and the Environment

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Phytate-degrading enzymes, also known as phytases, have a wide distribution in plants, microorganisms and some animal tissues (Konietzny and Greiner, 2002; see Hill and Richardson, Chapter 5, and Mullaney and Ullah, Chapter 7, this volume). They belong to a special class of phosphomonoesterases termed myo-inositol hexakisphosphate phosphohydolases, which are capable of initiating the stepwise release of phosphate residues from phytate (salts of myo-inositol hexakisphosphate), the major storage form of phosphate in plant seeds and pollen. The ability of such enzymes to hydrolyse phytate is usually known only from in vitro assays, and information on their in vivo function is rather limited. As enzymes are classified in general by their in vivo function, the term 'phytate-degrading enzyme' is preferred here to the term 'phytase', and is used throughout the chapter. The classification of enzymes as phytases based solely on in vitro assays becomes problematic when considering enzymes such as glucose-1-phosphatase in Escherichia coli and Enterobacter cloacae. This enzyme can hydrolyse phytate, albeit slowly, in vitro even though this is clearly not its in vivo function. Unless otherwise stated, designation of an enzyme as 'phytatedegrading' is based on in vitro assay using soluble sodium phytate.

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Influence of culture conditions on the biosynthesis of Schwanniomyces castellii phytase

Abstract: The influence of several factors on the biosynthesis of Schwanniomyces castellii phytase was studied in continuous culture. The level of phytase production increased with pH and dilution rate. It decreased when the phytic acid or phosphate content increased.

Cited by 28 publications

References 7 publications

Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Phytate-degrading enzymes: regulation of synthesis in microorganisms and plants.

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