Utilization of natural electrical charges on airborne microorganisms for their collection by electrostatic means

Yao, Maosheng; Mainelis, Gediminas

doi:10.1016/j.jaerosci.2005.05.006

Cited by 46 publications

(41 citation statements)

References 22 publications

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“…The evaluations followed a commonly used protocol (Yao and Mainelis 2006) with an experimental setup which is shown schematically in Figure S3. First, a downstream particle concentration C(V = 0,Q) was measured by the optical particle counter without applying the collection voltage.…”

Section: Evaluation Of the Physical Capture Efficiencymentioning

confidence: 99%

“…Compared to collection by impaction methods, advantages of the electrostatic-sampling-based method are lower impaction stress (Mainelis et al 1999), lower pressure drop and lower power consumption (Tan et al 2011). Yao demonstrated that electrostatic sampling could achieve culturable bioaerosol concentration levels as much as nine times higher than the BioStage impactor (Yao and Mainelis 2006) and five to ten times higher airborne allergen and toxin concentration levels than the BioSampler (Yao et al 2009). High concentration rates were also obtained with a smart implementation of the Lotus effect (Barthlott and Neinhuis 1997;Feng et al 2002) with an ultra hydrophobic surface (Han and Mainelis 2008;Han et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of a New Electrostatic Sampler for Concentrating Biological and Non-Biological Aerosol Particles

Roux

Kaspari

Heinrich

et al. 2013

Aerosol Science and Technology

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The detection of airborne pathogens is becoming a subject of great concern in modern day society. Recent studies have shown that electrostatic samplers are suitable for collecting microorganisms as well as preserving their viability. In most of these studies, flow rates were lower than 12.5 L/min or required a concentration stage to increase the flow rate to 100 L/min. In the present study, a single stage electrostatic sampler was developed for an efficient collection of microorganisms at 100 L/min. The design is based on the positive DC corona between coaxial cylinders to continuously create charged particles as they pass through the sampler. The physical collection efficiency of the device was investigated by sampling the ambient air particles. The efficiency in collecting live biological samples was determined by sampling live bacterial spores with the collector situated inside an aerosol chamber. It is shown that particles of 0.3-0.35 µm are captured with an efficiency of about 86%, whereas cultivable spores of Bacillus thuringiensis are collected with an efficiency of about 94%. Results are compared with an analytical predictive model. The experimental results are similar to the efficiencies previously reported in the literature; however, the current sampler design features a higher flow rate which enables the device to be used as an alarm trigger in a shorter period of time.

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Section: Evaluation Of the Physical Capture Efficiencymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of a New Electrostatic Sampler for Concentrating Biological and Non-Biological Aerosol Particles

Roux

Kaspari

Heinrich

et al. 2013

Aerosol Science and Technology

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show abstract

“…Their results indicated that the RCS High Flow obtained lower culturable counts than that of the BioSampler due to its higher desiccation factors ). Among various bioaerosol samplers, the electrostatic sampler is increasingly being applied to detect biological aerosols (Yao and Mainelis 2006c;Yao et al 2009b;Han et al 2010). It was shown that when the electrostatic sampler was used, the culturable bioaerosol concentrations could be 5-9 times higher than those obtained by the BioStage impactor (Yao and Mainelis 2006c).…”

Section: Introductionmentioning

confidence: 99%

“…Among various bioaerosol samplers, the electrostatic sampler is increasingly being applied to detect biological aerosols (Yao and Mainelis 2006c;Yao et al 2009b;Han et al 2010). It was shown that when the electrostatic sampler was used, the culturable bioaerosol concentrations could be 5-9 times higher than those obtained by the BioStage impactor (Yao and Mainelis 2006c). It was suggested that the electrostatic sampler introduced less impaction and desiccation stress than the BioStage impactor (Yao and Mainelis 2006c).…”

Section: Introductionmentioning

confidence: 99%

“…It was shown that when the electrostatic sampler was used, the culturable bioaerosol concentrations could be 5-9 times higher than those obtained by the BioStage impactor (Yao and Mainelis 2006c). It was suggested that the electrostatic sampler introduced less impaction and desiccation stress than the BioStage impactor (Yao and Mainelis 2006c). In the meantime, filter sampling including gelatin filter was also investigated for collecting bioaerosols in numerous studies (Wang et al 2001;Burton et al 2005;Chen and Li 2005;Nehme et al 2008;Droogenbroeck et al 2009;Yao et al 2009a;Wu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Culturable Bacterial and Fungal Aerosol Diversity Obtained Using Different Samplers and Culturing Methods

Yao

2011

Aerosol Science and Technology

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In this study, biological collection efficiencies and culturable bacterial and fungal aerosol diversities were investigated when different bioaerosol sampling tools and culturing methods were applied. The samplers included Reuter centrifugal sampler (RCS) High Flow, BioSampler, electrostatic sampler, gelatin filter, BioStage impactor, mixed cellulose ester (MCE) filter as well as gravitational settling methods. For culturable bacterial aerosol diversity, the colony-forming units (CFUs) were washed off from the agar plates, and further went through polymerase chain reactionand denaturing gradient gel electrophoresis (PCR-DGGE). For culturable fungal aerosol diversity, microscopic identification method was applied.In general, the BioStage impactor, MCE filter, and the BioSampler remained robust when sampling culturable bioaerosols. The PCR-DGGE study revealed that the use of different samplers and culturing methods resulted in different culturable bioaerosol diversity. For indoor bacterial aerosols, the MCE filter with direct culturing on agar plates and the BioSampler resulted in the highest number of visible bands on DGGE gels, followed by the gravitational settling (26 • C), the RCS High Flow, electrostatic sampler, and the MCE filter extracted. However, for outdoor bacterial aerosols, the BioStage impactor obtained the highest number of visible bands followed by the BioSampler, MCE filter, electrostatic sampler, the RCS High Flow, and gravitational settling at 26• C. When sampling fungal aerosols, the electrostatic sampler was observed to produce the highest diversity. Similar to previous studies, Alternaria, Cladosporium, and Aspergillus species were found to dominate the fungal community in most samples collected here.The results obtained here suggested that the sampler design, sampling environments, and culturing methods should be considered together when assessing the biological aerosol exposure.

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Biological Particle Sampling

Reponen

Willeke

Grinshpun

et al. 2011

Aerosol Measurement

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Utilization of natural electrical charges on airborne microorganisms for their collection by electrostatic means

Cited by 46 publications

References 22 publications

Investigation of a New Electrostatic Sampler for Concentrating Biological and Non-Biological Aerosol Particles

Investigation of a New Electrostatic Sampler for Concentrating Biological and Non-Biological Aerosol Particles

Analysis of Culturable Bacterial and Fungal Aerosol Diversity Obtained Using Different Samplers and Culturing Methods

Biological Particle Sampling

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