Utilization of Methylthio-s-Triazine for Growth of Soil Fungi 1

Rieck, W.; Lynd, J. Q.

doi:10.1128/aem.19.1.11-13.1970

Cited by 5 publications

(3 citation statements)

References 2 publications

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“…1). None of these compounds is known to be a carbon or nitrogen source for growth [18,50] (Table 1), so enrichments were prepared to test the hypothesis [93] that SIIT and SILT are sulphur sources for growth [50].…”

Section: Enrichments With a Sulphur Limitationmentioning

confidence: 99%

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Biodegration of s-triazine xenobiotics

Cook

1987

FEMS Microbiology Letters

206

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SUMMARYBiodegradation of xenobiotic compounds is examined with s-triazines as an example and with biological treatment of wastewater containing striazines as an aim. s-Triazines have been termed recalcitrant, but examination of the literature indicates that a potential for biodegradation exists. Nitrogen-limited enrichment cultures yield organisms able to degrade by-products of the industrial synthesis of s-triazine herbicides as sources of nitrogen. The choice of inoculum for these enrichments is important and often allows for successful enrichment after simple batch culture, but organisms containing several degradative reactions could be obtained only after selection in extended culture. Routine, specific determinations of all s-triazines (by HPLC) were essential throughout the work. Molar growth yields show complete mass balances for the utilization of s-triazines. Kinetic experiments indicate that specific degradation rates of s-triazines in growing cells are about 0.4 mkat/kg of protein. Characterised biochemical pathways consist of a series of hydrolytic cleavages of chloro-, amino-and alkylaminogroups from the s-triazine ring. Pathways con-

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Section: Enrichments With a Sulphur Limitationmentioning

confidence: 99%

“…These organisms were obtained only from inocula with an extensive history of exposure to striazines [50]. The claim [93] that a culture-collection strain of Aspergillus niger [96] utilizes SIIT as a sulphur source appears to be due to utilisation of traces of contaminant sulfur [50] (section 5.1.3).…”

Section: Enrichments With a Sulphur Limitationmentioning

confidence: 99%

Biodegration of s-triazine xenobiotics

Cook

1987

FEMS Microbiology Letters

206

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“…1) were investigated because they are widely used herbicides and are available in highly pure form. There is a claim (21) for the fungal utilization of prometryne. The data, however, do not appear to verify the claim, because the molar growth yields with sulfate and prometryne as sulfur sources differed by a factor of about 150, and the amount of prometryne supplied was apparently eightfold lower than the amount measured as disappearing during the incubation.…”

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confidence: 99%

Ametryne and Prometryne as Sulfur Sources for Bacteria

Cook

Hütter

1982

Appl Environ Microbiol

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Bacteria were isolated that could utilize quantitatively the s-triazine herbicide prometryne [N,N'-bis(1-methylethyl)-6-(methylthio)-1,3,5-triazine-2,4-diamine] or ametryne [N-ethyl-N'-(1-methylethyl)-6-(methylthio)-1,3,5-triazine-2,4-diamine], or both, as a sole source of sulfur for growth. The success of enrichments depended on previous exposure of the soil inoculum to s-triazine herbicides. Deaminoethylametryne [4-(1-methylethyl)amino-6-(methylthio)-1,3,5-triazine-2-(lH)-one], methylsulfonic acid, and sodium sulfate could also be used as sulfur sources. Utilization of a compound was quantified as the growth yield per mole of sulfur supplied. Yields were about 6 kg of protein per mol of sulfur. The product of the desulfuration of an striazine was identified as the corresponding hydroxy-derivative. This is the first substantiated report of the utilization of these s-triazines as sulfur sources by bacteria.

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