Utilization of Enzymatically Hydrolyzed Soybean Protein and Crystalline Amino Acid Diets by Rats with Exocrine Pancreatic Insufficiency

Imondi, A. R.; Stradley, R.P.

doi:10.1093/jn/104.6.793

Cited by 24 publications

(13 citation statements)

References 24 publications

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“…It is also suggested that enzymatically hydrolyzed proteins are utilized more efficiently than mixtures of amino acids in the absence of exocrine pancreatic function [30]. Based on our data, SP is absorbed more rapidly than is LP by the jejunum of the normal rat intestine, and the peptides of a soybean hydrolysate are absorbed effectively as a source of nitrogen in the rat small intestine.…”

Section: Effects Of Cpm On Mucosal Parametersmentioning

confidence: 57%

Effect of Peptide Chain Length on Absorption and Intact Transport of Hydrolyzed Soybean Peptide in Rat Intestinal Everted Sac.

Chun

Sasaki

Fujiyama

et al. 1996

J. Clin. Biochem. Nutr.

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SummaryIn this study using everted sacs of rat jejunum, we investigated the difference in absorption of two types of soybean peptides (small peptide: SP, and large peptide: LP). We investigated the influence of peptide length on absorption and intact transport of hydrolyzed soybean peptides. The everted sacs were incubated for S or 10 min in bicarbonate-saline buffer containing a 1% (w/v) concentration of each soybean peptide. After the incubation, the amounts of free amino acids and peptides that were transported to the serosal fluids and the mucosa were measured with an automatic amino acid analyzer both before and after the hydrolysis of the serosal fluids and mucosa. The results indicated that SP was absorbed more rapidly than LP and that the more rapid absorption of SP was due to the greater intact transport of SP. When the everted sacs were made from jejunum that had been injured by intraperitoneal injection of cyclophosphamide (300 mg/kg), no significant differences were noted between the absorption of SP and that of LP. We also ascertained that an aminopeptidase inhibitor (bestatin) decreased the aminopeptidase activities of the rat jejunum. In the presence of 1 X 10_4 M bestatin in the incubation buffer, no significant differences were noted between the absorption of SP and that of LP. We conclude that SP is absorbed more rapidly than LP in normal rat jejunum due to greater intact transport.

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Section: Effects Of Cpm On Mucosal Parametersmentioning

confidence: 57%

Effect of Peptide Chain Length on Absorption and Intact Transport of Hydrolyzed Soybean Peptide in Rat Intestinal Everted Sac.

Chun

Sasaki

Fujiyama

et al. 1996

J. Clin. Biochem. Nutr.

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“…29 Recent evidence in both animal and human studies suggests that elemental or semi-elemental small peptide formulas are well tolerated, and efficiently absorbed in the gut lumen with little or no pancreatic enzyme secretion. [30][31][32] Previous studies have shown that jejunal feeding results in negligible increases in pancreatic enzyme, bicarbonate, and volume output from the pancreas. 33,34 Similarly, the present study showed that jejunal administration of small peptide formulas was well tolerated in APinduced rats.…”

Section: Commentmentioning

confidence: 99%

Enteral Nutrition Prevents Bacterial Translocation but Does Not Improve Survival During Acute Pancreatitis

Kotani

Usami²,

Nomura³

et al. 1999

Arch Surg

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To evaluate the effect of enteral nutrition (EN) in attenuating bacterial and/or endotoxin translocation, maintaining immune responsiveness, and improving outcome in early acute pancreatitis (AP) in Wistar male rats. Design: Acute pancreatitis was induced in rats receiving total parenteral nutrition (TPN) (AP/TPN group) (n=34) and EN (AP/EN group) (n=35) by pressure injection of 1% deoxycholate into the biliopancreatic duct (0.6 mg/kg of body weight). Rats in the sham/ TPN and sham/EN groups (n=10 each) underwent laparotomy without induction of AP. Catheters for TPN and EN were placed into the external jugular vein and jejunum, respectively. Rats were infused with Ringer lactate solution for 48 hours followed by TPN in the AP/TPN and sham/TPN groups, and EN in the AP/EN and sham/EN groups until day 7. The fluid volume and energy (calories) intake were similar in all groups. Setting: Medical school research laboratory. Main Outcome Measures: Survival, blood endotoxin level, villus height, 5-bromo-2'-deoxyuridine (BrdU) uptake in the jejunum and ileum, bacterial culture of mes-enteric lymph nodes, and CD4/CD8 ratio of T cells in mesenteric lymph nodes, spleen, and peripheral blood. Results: There was no difference in survival and pancreatic healing between the AP/TPN and AP/EN groups. Colony-forming units of the mesenteric lymph nodes and the endotoxin level were significantly lower in the AP/EN group than in the AP/TPN group (PϽ.05). Villus height and BrdU intake was significantly higher in the AP/EN group than in the AP/TPN group (PϽ.05). The CD4/CD8 ratio of T cells in spleen and peripheral blood was higher in the AP/EN group than in the AP/TPN group (PϽ.05), whereas there was no difference in mesenteric lymph nodes.

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confidence: 99%

“…In both animal and human studies, elemental or semi-elemental small peptide formulas have been shown to be well tolerated and efficiently absorbed in a gut lumenal environment with little or no pancreatic enzyme secretion. [10][11][12][13] Case series have documented that TEN could be used safely as transitional feeding in patients who had peaked and had begun to resolve the inflammatory response associated with pancreatitis. [14][15][16][17][18][19][20][21] The potential advantages of TEN over TPN and the feasibility that TEN administered via jejunal feedings might be well tolerated prompted us to set up this prospective randomized trial comparing early TEN to TPN in acute pancreatitis.…”

mentioning

confidence: 99%

Comparison of the Safety of Early Enteral vs Parenteral Nutrition in Mild Acute Pancreatitis

McClave

Greene

Snider

et al. 1997

J Parenter Enteral Nutr

397

215

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Utilization of Enzymatically Hydrolyzed Soybean Protein and Crystalline Amino Acid Diets by Rats with Exocrine Pancreatic Insufficiency

Cited by 24 publications

References 24 publications

Effect of Peptide Chain Length on Absorption and Intact Transport of Hydrolyzed Soybean Peptide in Rat Intestinal Everted Sac.

Effect of Peptide Chain Length on Absorption and Intact Transport of Hydrolyzed Soybean Peptide in Rat Intestinal Everted Sac.

Enteral Nutrition Prevents Bacterial Translocation but Does Not Improve Survival During Acute Pancreatitis

Comparison of the Safety of Early Enteral vs Parenteral Nutrition in Mild Acute Pancreatitis

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