Utilization of Citric Acid by some So-called Citrate-non-Utilizing Bacteria

Lominski, Iwo; Conway, Nancy S.; Harper, Elizabeth M.; Rennie, J. Basil

doi:10.1038/160573b0

Cited by 19 publications

(8 citation statements)

References 2 publications

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“…As citrate utilization had been reported to be difficult in this species of microorganism (1), the situation is quite similar to the case of E. coli mentioned previously (4,5). Formate and glycollate were found not to be metabolized even with the addition of auxiliary nutrients.…”

Section: Resultssupporting

confidence: 64%

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Utilization of Organic Acids for the Glutamic Acid Production by Micrococcus Glutamicus

Kimura¹

1964

J. Gen. Appl. Microbiol.

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Section: Resultssupporting

confidence: 64%

“…For example, Escherichia coli which was known to be unable to utilize citric acid in KOSER's medium was later found to be able to metabolize it when the medium was supplemented with a small amount of a nutrient such as peptone, glucose or acetate (4,5).…”

Section: Assimilationmentioning

confidence: 99%

Utilization of Organic Acids for the Glutamic Acid Production by Micrococcus Glutamicus

Kimura¹

1964

J. Gen. Appl. Microbiol.

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“…On the other hand, all Shigella species and three Proteus species did not cause clotting. Lominski, Conway, Harper & Rennie (1947) Corda (1947) disagreed with this basis for differentiation because of the instability of the character. Our experiments also showed the unreliability of this criterion since results occasionally varied with the batch of plasma used.…”

Section: Discussionmentioning

confidence: 99%

Clotting of Citrated Plasma and Citrate Utilization by Intestinal Gram-negative Bacilli

Mushin

Kerr

1954

Journal of General Microbiology

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SUMMARY : Salmonella, shigella, alkalescens-dispar, proteus, paracolon and coliform strains were tested for the presence of a coagulase enzyme as manifested by the ability to clot plasma. The shigella and proteus strains examined gave negative results ; a high percentage of the remaining strains clotted citrated plasma but this reaction was due to metabolism of citrate and subsequent liberation of calcium ions and not to a coagulase enzyme. Results depended on the bacterial strain and on the kind and dilution of plasma used. Utilization of citrate in citrated plasma was compared with the ability of the tested strains to attack citrate in Koser's ammonium citrate medium and in sodium citrate peptone medium. The Shigella and Proteus species examined failed to utilize citrate in any of the media under the conditions of the tests, Salmonella species gave fairly uniform results in the three media, while paracolon, coliform and alkalescens-dispar strains differed in their activity, the highest percentage of positive reactions being obtained in plasma-containing media.In the course of a study of Gram-negative bacilli these organisms were examined to see whether they possessed coagulases capable of clotting plasma. A survey of literature showed that numerous bacterial species have been examined for plasma-coagulating ability. Conflicting results have been obtained, due possibly to different experimental conditions. In the present communication only intestinal Gram-negative bacilli will be considered.Loeb (

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“…coli to oxidize citrate, failure to dissimilate this compound may be due also to lack of citridesmolase development in cells grown in citrate +mineral salt media. Lominski, Conway, Harper & Rennie (1947) and Vaughn, Osborne, Wedding, Tabachnick, Beisel & Braxton (1950) reported disappearance of citrate from cultures of E . coli supplied with various nutrients, while Grunberg-Manago & Gunsalus (1953) prepared extracts of the organism which dissimilated citrate.…”

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confidence: 99%

Dissimilation of Citric Acid by Aerobacter aerogenes and Escherichia coli

Dagley

1954

Journal of General Microbiology

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SUMMARY: When Aerobacter aerogenes was harvested from citrate + mineral salt media in which the concentration of carbon source limited the crop, citridesmolase activity of cells was high but was absent when growth ceased with citrate in excess. These differences were abolished by disintegration of the cells. The ability of Escherichia coli to dissimilate citrate was measurable only after growth in a medium containing peptone, citrate and glucose : when the latter was consumed activity fell and was revived by further glucose additions. Differences in activity of such cells were not abolished by their disintegration. Cells lost their activity when incubated for 30 min. with phosphate buffer or with the citrate +mineral salt medium that supports growth of A. aerogenes. E. cold grown at the expense of glucose in a mineral salt medium+citrate, did not utilize citrate for growth during the first day and no citridesmolase activity could be detected, but when subsequently tested in a medium which permitted development of activity, higher activities were reached by cells that had grown in presence of citrate. E. co2i grown in peptone + citrate +glucose medium possessed little oxaloacetate decarboxylase ; by the action of disintegrated cells, citrate gave rise to equimolar quantities of keto-acid, chiefly oxaloacetic acid.

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Utilization of Citric Acid by some So-called Citrate-non-Utilizing Bacteria

Cited by 19 publications

References 2 publications

Utilization of Organic Acids for the Glutamic Acid Production by Micrococcus Glutamicus

Utilization of Organic Acids for the Glutamic Acid Production by Micrococcus Glutamicus

Clotting of Citrated Plasma and Citrate Utilization by Intestinal Gram-negative Bacilli

Dissimilation of Citric Acid by Aerobacter aerogenes and Escherichia coli

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