Utilization of cell culture techniques in carbaryl metabolism studies

Carbaryl: A Literature Review

“…Their work indicated the 4-hydroxycarbaryl accounted for 16.6 and 5.2% of the carbaryl dose in the perfusate and lung tissue, respectively. This was in agreement with BARON and LOCKE (1970) and LIN et al (1975), who found carbaryl metabolized to 4-hydroxycarbaryl and its conjugates by cultured lung cells.…”

“…Metabolism.- BARoN and LOCKE (1970) studied the degradation produets of carbaryl added to human normal embryonic lung. Carbaryl was eompletely metabolized within 3 days, suggesting hydroxylation at the C4-position and hydrolysis of the earbamate group.…”

Studies of carbamate pesticide metabolism utilizing plant and mammalian cells in culture

“…The remaining label contained in the supernatant fraction consisted of an unidentified organoextractable metabolite, neutral conjugates .of carbaryl, anaphthol, and 5,6-dihydro-5,6-dihydroxycarbaryl, and acidic conjugates of carbaryl and a-naphthol.Cell culture techniques have previously been used in studies of pesticide toxicity (Gabliks and Friedman, 1969;Litterest et al, 1969) and, more recently, in studies of pesticide metabolism (Huang et al, 1970). Previous studies from this laboratory on carbaryl metabolism by human embryonic lung cells in culture have shown that cell culture techniques are potentially useful for preliminary studies of pesticide degradation (Baron and Locke, 1970). The same techniques were used to study Banol (2-chloro-4,5-dimethylphenyl A-methylcarbamate) and UC-34096 [A'-Carbaryl (1-naphthyl A-methylcarbamate) metabolism by tobacco cells in suspension culture was also investigated.The metabolite that is produced from Banol in the lung cell system reflects a classical mammalian route of detoxification; it may represent a minor metabolite in the rat (Baron and Doherty, 1967).…”

“…Plates were developed with one of the following solvent systems: A. etherhexane (1:1, v/v); B. acetonitrile-hexane (9:1, v/v); C. methyl ethyl ketone-acetone-water (9.3:2.6:1.0, v/v/v); and D. isopropyl alcohol-concentrated ammonium hydroxidewater (7:1:2, v/v/v).Liquid Scintillation Counting. All radioactivity measurements were made with a Packard TriCarb liquid scintillation spectrometer, Model 3365, using a scintillation phosphor previously described (Baron and Locke, 1970).…”

“…Cell culture techniques have previously been used in studies of pesticide toxicity (Gabliks and Friedman, 1969;Litterest et al, 1969) and, more recently, in studies of pesticide metabolism (Huang et al, 1970). Previous studies from this laboratory on carbaryl metabolism by human embryonic lung cells in culture have shown that cell culture techniques are potentially useful for preliminary studies of pesticide degradation (Baron and Locke, 1970). The same techniques were used to study Banol (2-chloro-4,5-dimethylphenyl A-methylcarbamate) and UC-34096 [A'-…”