Utilization of biosolids for glucose oxidase production: A potential bio-fenton reagent for advanced oxidation process for removal of pharmaceutically active compounds

Vaithyanathan, Vasanth Kumar; Ravi, Shobana; Leduc, Roland; Vaidyanathan, Vinoth Kumar; Cabana, Hubert

doi:10.1016/j.jenvman.2020.110995

Cited by 15 publications

(9 citation statements)

References 36 publications

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“…In basidiomycetes, like P. dryinus, the extracellular cellular enzyme produced during fermentation closely binds to the mycelial hyphal structure as their interlinkage association is strong (Bentil et al, 2018). In addition, fungi that grow over BS form an agglomeration, which generates a floc-like formation where the secreted enzymes immobilize to it (Vaithyanathan et al, 2020). The enzyme binds strongly to the substrate during hydrolysis, making it difficult to recover by normal washing (Bentil et al, 2018).…”

Section: Enzyme Recovery Using Ultrasonicationmentioning

confidence: 99%

Laccase-Driven Transformation of High Priority Pesticides Without Redox Mediators: Towards Bioremediation of Contaminated Wastewaters

Vaithyanathan

Vaidyanathan

Cabana

2022

Front. Bioeng. Biotechnol.

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In this study, Pleurotus dryinus was grown on municipal biosolids (BS) as the substrate to produce laccase for the removal of pesticides (fungicides, herbicides, and insecticides) from wastewater. Among the various types of BS tested, sterilized biosolids were the most promising substrate for laccase production by P. dryinus with a maximal laccase activity (162.1 ± 21.1 U/g dry substrate), followed by hygenized biosolids (96.7 ± 17.6 U/g dry substrate), unsterilized biosolids (UBS) (31.9 ± 1.2 U/g dry substrate), and alkali-treated biosolids (8.2 ± 0.4 U/g dry substrate). The ultrasound-assisted extraction of this enzyme from fermented UBS was carried out with 0.1 M phosphate buffer at pH 7.0, which increased the enzyme activity of the crude extract by 30%. To test the catalytic potential of the biocatalyst in real matrices, 1 U/ml of recovered crude laccase extract was applied for 24 h for the removal of 29 pesticides (nine fungicides, 10 herbicides, and 10 insecticides) either separately or as a mixture from spiked biologically treated wastewater effluent. When treated with crude enzyme extract, high-priority herbicides metolachlor and atrazine were completely removed, while 93%–97% of the insecticides aldicarb, spinosad, and azinphos-methyl and up to 91% of kresoxim-methyl were removed. Promising results were obtained with BS-derived crude enzyme extract exhibiting improved pesticides removal, which may be due to the mediator effect resulting from the catalytic transformation of other molecules in the cocktail. The results demonstrated a promising integrated bioprocess for the removal of pesticides in wastewater using crude laccase obtained from BS.

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Section: Enzyme Recovery Using Ultrasonicationmentioning

confidence: 99%

Laccase-Driven Transformation of High Priority Pesticides Without Redox Mediators: Towards Bioremediation of Contaminated Wastewaters

Vaithyanathan

Vaidyanathan

Cabana

2022

Front. Bioeng. Biotechnol.

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“…The VAPs obtained from the sludge valorization using aerobic bioaugmentation are listed in Table 3. The promising VAPs are biodiesel from Trichosporon oleaginosus (Zhang et al, 2014;Chen et al, 2021), enzymes from Aspergillus niger (Vaithyanathan et al, 2020), T. versicolor (Rodríguez-Rodríguez et al, 2011Llorens-Blanch et al, 2018) and Bacillus subtilis (Vaithyanathan et al, 2021b), biopesticide from B. thuringiensis, (Balasubramanian and Tyagi, 2017a;Cerda et al, 2019) and biosurfactant from Starmerella bombicola (Cerda et al, 2019). These products have future applications in extraction and production, and thus, provide a way for waste disposal and cost-effective production of VAPs.…”

Section: Aerobic Digestionmentioning

confidence: 99%

“…Glucose oxidase (GOD) is one of the most important enzymes that can be produced by using this nutritious biomass from sludge. Vaithyanathan et al (2020) proposed a cost-effective process to produce GOD using BS as a substrate by employing A. niger. A maximum GOD activity of 6012 U/L was achieved in 48 h when 72-h old inoculum of 20% (w/v) inoculum size was used in 25% (dw/v) BS media.…”

Section: Enzymesmentioning

confidence: 99%

“…A maximum GOD activity of 6012 U/L was achieved in 48 h when 72-h old inoculum of 20% (w/v) inoculum size was used in 25% (dw/v) BS media. This approach can also simultaneously improve recalcitrant organic compound removal in treatment plants (Vaithyanathan et al, 2020).…”

Section: Enzymesmentioning

confidence: 99%

“…Apart from their nutrient content, BS are also rich in microbial sources, pathogens, organic and inorganic contaminants (Marguí et al, 2016;Fijalkowski et al, 2017). There are many published works that describe the production of these solid wastes around the globe (Verlicchi and Zambello, 2015;Kumar et al, 2017;Sharma et al, 2017;Gao et al, 2020;Mohajerani and Karabatak, 2020;Vaithyanathan et al, 2020). The amount of BS produced annually worldwide has increased dramatically due to the construction of treatment plants and upgrading of existing facilities as a result of increased water demand and WWTP regulations (Suksiripattanapong et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Integrated Biotechnology Management of Biosolids: Sustainable Ways to Produce Value—Added Products

Vaithyanathan

Cabana

2021

Front. Water

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Biosolids (BS) are organic dry matter produced from wastewater treatment plants (WWTPs). The current yearly worldwide production of BS is estimated to be around 100–125 million tons and is expected to continuously increase to around 150–200 million tons by 2025. Wastewater treatment industries across the globe strive to achieve a green and sustainable manufacturing base for the management of enormous amounts of municipal BS, which are rich in nutrients and organic dry matter along with contaminants. The management of these organic-rich wastes through environmentally friendly recovery technologies is a major challenge. The need to improve waste biomass disposal by biological development and develop more economically viable processes has led to a focus on the transformation of waste resources into value-added products (VAP). This paper assesses the leading disposal methods (based on volume and contaminant reduction) and reviews the state of biotechnological processes for VAP recovery from municipal wastewater sludge (untreated solid waste residual) and BS (stabilized solid waste which meets criteria for its use in land). A review of the anaerobic and aerobic digestion processes is presented to provide a holistic overview of this growing research field. Furthermore, the paper also sheds light on the pollutant reduction and resource recovery approaches for enzymes, bioflocculants, bioplastics, biopesticides, and biogas as a mean to represent BS as a potential opportunity for WWTPs. However, only a few technologies have been implemented for VAP resource recovery and a shift from WWTPs to waste resource recovery facilities is still far from being achieved.

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Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

Zhou,

Zhang,

Qian

2023

Biotech & Bioengineering

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Glucose oxidase (EC 1.1.3.4, GOD) is a widely used industrial enzyme. To construct a GOD‐hyperproducing Pichia pastoris strain, combinatorial strategies have been applied to improve GOD activity, synthesis, and secretion. First, wild‐type GOD was subjected to saturation mutagenesis to obtain an improved variant, MGOD1 (V20W/T30S), with 1.7‐fold higher kcat/KM. Subsequently, efficient signal peptides were screened, and the copy number of MGOD1 was optimized to generate a high‐producing strain, 8GM1, containing eight copies of AOX1 promoter‐GAS1 signal peptide‐MGOD1 expression cassette. Finally, the vesicle trafficking of 8GM1 was engineered to obtain the hyperproducing strain G1EeSe co‐expressing the trafficking components EES and SEC. 22, and the EES gene (PAS_chr3_0685) was found to facilitate both protein secretion and production for the first time. Using these strategies, GOD secretion was enhanced 65.2‐fold. In the 5‐L bioreactor, conventional fed‐batch fermentation without any process optimization resulted in up to 7223.0 U/mL extracellular GOD activity (3.3‐fold higher than the highest level reported to date), with almost only GOD in the fermentation supernatant at a protein concentration of 30.7 g/L. Therefore, a GOD hyperproducing strain for industrial applications was developed, and this successful case can provide a valuable reference for the construction of high‐producing strains for other industrial enzymes.

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Utilization of biosolids for glucose oxidase production: A potential bio-fenton reagent for advanced oxidation process for removal of pharmaceutically active compounds

Cited by 15 publications

References 36 publications

Laccase-Driven Transformation of High Priority Pesticides Without Redox Mediators: Towards Bioremediation of Contaminated Wastewaters

Laccase-Driven Transformation of High Priority Pesticides Without Redox Mediators: Towards Bioremediation of Contaminated Wastewaters

Integrated Biotechnology Management of Biosolids: Sustainable Ways to Produce Value—Added Products

Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

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