Utilization of antimicrobial peptides, analogues and mimics in creating antimicrobial surfaces and bio-materials

Pinto, Ingrid Batista; Machado, Leandro dos Santos; Meneguetti, Beatriz T.; Nogueira, Micaella Lima; Carvalho, Cristiano Marcelo Espínola; Roel, Antônia Railda; Franco, Octávio L.

doi:10.1016/j.bej.2019.107237

Cited by 22 publications

(14 citation statements)

References 196 publications

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“…AMPs have attracted considerable interest for the design of antibacterial coatings [13][14][15][22][23][24], but some aspects, particularly longer-term performance, are still in need of further study, as is the question of whether some AMPs might be less effective due to conformational changes or accessibility after grafting with specific immobilization chemistries. Their potential use as covalently grafted protective coatings needs to be informed by considerations such as mechanism(s) of action and possible cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

“…To retain AMPs on surfaces of biomedical devices in biological environments, they must be bound covalently ("grafted") to the biomaterial surface. There are many reports on grafting of AMPs onto various materials by various interfacial linking chemistries, as discussed in recent reviews [22][23][24]. In this study, we have utilized the approach of using a plasma polymer interlayer bearing surface aldehyde groups that can react with amine groups of AMPs to form an interfacial covalent bond [25].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

et al. 2021

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To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including coatings comprising naturally occurring antimicrobial peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were selected on the basis of well-known membrane disruption activity in solution and were covalently grafted onto a plasma polymer platform, which enables application of this multilayer coating strategy to a wide range of biomaterials. Detailed surface analyses were performed to verify the intended outcomes of the coating sequence. Samples were challenged by incubation in bacterial growth media for 5 and 20 h. Compared with the control plasma polymer surface, all three grafted AMP coatings showed considerable reductions in bacterial colonization even at the high bacterial challenge of initial seeding at 1 × 107 CFU, but there were increasing numbers of dead bacteria attached to the surface. All three grafted AMP coatings were found to be non-toxic to primary fibroblasts. These coatings thus could be useful to produce antibacterial surface coatings for biomaterials, though possible consequences arising from the presence of dead bacteria need to be studied further, and compared to non-fouling coatings that avoid attachment of dead bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

et al. 2021

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show abstract

“…AMPs have attracted considerable interest for the design of antibacterial coatings, [13][14][15][22][23][24] but some aspects, particularly longer-term performance, are still in need of further study, as is the question of whether some AMPs might be less effective due to conformational changes or accessibility after grafting with specific immobilization chemistries. Their potential use as covalently grafted protective coatings needs to be informed by considerations such as mechanism(s) of action and possible cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

“…There are many reports on grafting of AMPs onto various materials by various interfacial linking chemistries, as discussed in recent reviews. [22][23][24] In this study, we have utilized the approach of using a plasma polymer interlayer bearing surface aldehyde groups that can react with amine groups of AMPs to form an interfacial covalent bond. [25] The attraction of using a plasma polymer interlayer is that identical plasma polymers can facilely be deposited onto a wide range of materials and devices, and hence our coating strategy is generically applicable to a wide range of potential products.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

Griesser¹,

Jasieniak²,

Vasilev³

et al. 2020

Preprint

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To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including coatings comprising naturally occurring antimicrobial peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were covalently grafted onto a plasma polymer platform, which enables application of this multilayer coating strategy to a wide range of biomaterials. Detailed surface analyses were performed to verify the intended outcomes of the coating sequence. Samples were challenged by incubation in bacterial growth media for 5 and 20 hrs. Compared with the control plasma polymer surface, all three grafted AMP coatings showed considerable reductions in bacterial colonization even at the high bacterial challenge of initial seeding at 1x107 CFU, but there were increasing numbers of dead bacteria attached to the surface. All three grafted AMP coatings were found to be non-toxic to primary fibroblasts. These coatings thus could be useful to produce antibacterial surface coatings for biomaterials, though possible consequences arising from the presence of dead bacteria need to be studied further, and compared to non-fouling coatings that avoid attached dead bacteria.

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“…Antimicrobial peptides (AMPs) represent a promising alternative to conventional antibiotics to reduce the incidence of MDIs [ 103 ]. AMPs are small-sized (12–50 amino acids) amphipathic cationic defense molecules widely diffused in nature, which can be found in four structural conformations (α-helix, β-sheet, extended helix, and loops) [ 104 ]. Cathelicidins (LL-37), human β-defensins 3 (HBD-3), magainins, and temporins are the most prevalent AMPs.…”

Section: Promising Antimicrobial Compounds For the Treatment Of Cardiac And Orthopaedic Device-associated Infectionsmentioning

confidence: 99%

Trends in Managing Cardiac and Orthopaedic Device-Associated Infections by Using Therapeutic Biomaterials

et al. 2021

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Over the years, there has been an increasing number of cardiac and orthopaedic implanted medical devices, which has caused an increased incidence of device-associated infections. The surfaces of these indwelling devices are preferred sites for the development of biofilms that are potentially lethal for patients. Device-related infections form a large proportion of hospital-acquired infections and have a bearing on both morbidity and mortality. Treatment of these infections is limited to the use of systemic antibiotics with invasive revision surgeries, which had implications on healthcare burdens. The purpose of this review is to describe the main causes that lead to the onset of infection, highlighting both the biological and clinical pathophysiology. Both passive and active surface treatments have been used in the field of biomaterials to reduce the impact of these infections. This includes the use of antimicrobial peptides and ionic liquids in the preventive treatment of antibiotic-resistant biofilms. Thus far, multiple in vivo studies have shown efficacious effects against the antibiotic-resistant biofilm. However, this has yet to materialize in clinical medicine.

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Utilization of antimicrobial peptides, analogues and mimics in creating antimicrobial surfaces and bio-materials

Cited by 22 publications

References 196 publications

Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

Trends in Managing Cardiac and Orthopaedic Device-Associated Infections by Using Therapeutic Biomaterials

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