Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation

Oliveira, Sérgio Dantas; Padilha, Carlos Eduardo de Araújo; Asevedo, Elson; Pimentel, Vanessa Carvalho; Araújo, Fernanda Rodrigues de; Macêdo, Gorete Ribeiro de; Santos, Everaldo Silvino dos

doi:10.1016/j.jece.2017.12.038

Cited by 40 publications

(22 citation statements)

References 31 publications

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“…The induction of cellulase induction is strongly influenced by carbon, nitrogen, and physical parameters such as pH, temperature, and incubation time [32]. The lack of an effect that drives the production of cellulase activity by the mineral media can be associated with a saturation of nutrients in the A. tubingensis NKBP-55 Copra meal 3.8 [52] T. asperellum MR 1 Petiole palm oil pressing 0.72 [53] Inonotus obliquus Wheat bread 3.16 [15] Aspergillus fumigatus Sugarcane bagasse 0.62 [54] A. tubingensis NRRL4700 Cynara cardunculus 4 medium. High concentrations, some minerals like Mn, Co, Zn, and Fe, can cause a decrease in cellulase activity yields [31].…”

Section: Evaluation Of the Use Of Mineral Enrichment Media In The Production Of Cellulase Enzymes Using Green Tea Residues As Supportmentioning

confidence: 99%

“…One of the strategies that can be applied for the use of this class of waste is its use for the production of compounds of interest, such as enzymes, through solid-state fermentation processes (SSF) [15]. SSF defines it as an aerobic fermentation process developed in the absence of free water but with sufficient moisture for microbial growth [16].…”

Section: Introductionmentioning

confidence: 99%

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Use of wastes from the tea and coffee industries for the production of cellulases using fungi isolated from the Western Ghats of India

Saldaña-Mendoza

Ascacio‐Valdés

Palacios-Ponce

et al. 2020

Syst Microbiol and Biomanuf

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In this study, coffee pulp (Coffea arabica) and green tea (Camellia sinensis) residues were characterized for use as a substrate of solid-state fermentation for cellulases production. The invasion rate was evaluated, as well as cellulases production by strains of Aspergillus niger and Trichoderma asperellum from the western Ghats of India, on coffee pulp, green tea, and a mixture of both substrates (50:50). T. asperellum (AFP) strain was found to have the highest growth rate (0.409 ± 0.021 mm/h) using a mixture of both substrates. The production of cellulases by T. asperellum was unsatisfactory due to the presence of polyphenols in the supports to which A. nigger cellulases are more resistant. The production of cellulases by A. nigger was linked to the pH of the supports, favouring the use of T and TC. It was found that the extracts produced by A. niger (28A strain using a mixture substrate, 28A, and 20A strains using only green tea as a substrate) presented the highest cellulase activities when evaluated using a plate technique producing degradation halos of 2.3 ± 0.1 cm of diameter. Aspergillus 28A strain did not require mineral enrichment media for cellulase production using green tea residues as support of solid-state fermentation.

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Section: Evaluation Of the Use Of Mineral Enrichment Media In The Production Of Cellulase Enzymes Using Green Tea Residues As Supportmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of wastes from the tea and coffee industries for the production of cellulases using fungi isolated from the Western Ghats of India

Saldaña-Mendoza

Ascacio‐Valdés

Palacios-Ponce

et al. 2020

Syst Microbiol and Biomanuf

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“…With the XRD data collection, the cellulose Ⅰ characteristic peak was near 2θ of 22.5° ([002] lattice plane), whereas the amorphous cellulose characteristic peak was near 2θ of 18°. To calculate the crystallinity index (CrI), the Origin 8.0 software (Rigaku, Tokyo, Japan) was used; the CrI equation is as follows (Agarwal et al 2017;Oliveira et al 2017),…”

Section: Pulp Testingmentioning

confidence: 99%

Understanding the effect of severity factor of prehydrolysis on dissolving pulp production using prehydrolysis kraft pulping and elemental chlorine-free bleaching sequence

Wei

Tian

et al. 2020

BioRes

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Prehydrolysis kraft pulping is an effective approach to produce dissolving pulp, which can be used for viscose application. The prehydrolysis process using hot liquid water could remove hemicellulose and loosen the compact cell wall, thus facilitating subsequent pulping and bleaching processes. In this study, the composite severity factor (CSF) was used to reveal the intensity of prehydrolysis treatment and its effect on the pulping and bleaching process by combining the temperature, time, and pH variables. Results showed that the optimum CSF was 6.61, which produced a pulp with α-cellulose of 92.3%, degree of polymerization (DP) of 1081, brightness of 85.1% ISO, and Kappa number of 0.61. In addition, the fiber quality, crystalline structure, and microstructure of pulps were characterized by FQA (fiber quality analysis), XRD (X-ray diffraction), and SEM (scanning electron microscopy).

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“…In addition, after SSF using SCB previously submitted to alkaline pretreatment, the enzymatic activities increased to 90. glucosidase after SSF, respectively. Oliveira et al [28] evaluated different substrates (sugarcane bagasse, green coconut husk and cashew apple bagasse) for production of cellulases by A. fumigatus.…”

Section: Production Of Enzymes By Isolated Ascomycetesmentioning

confidence: 99%

“…Um estudo comparando os extratos foi realizado aplicando a correlação linear de Pearson. As análises foram executadas no Action 2.9 (ANOVA) e Sisvar 5.6 (Scott Knott) Oliveira et al (2018). avaliaram diferentes biomassas na produção de celulases por A. fumigatus por FES e o melhor resíduo foi o bagaço de cana, na qual foram obtidas 4,20 e 0,64 U/g de CMCase e FPase, respectivamente.Portanto, os resultados obtidos no presente trabalho para produção de celulases pelas linhagens A. niger SCBM1(F1)e A. fumigatus SCBM6 (F2) isoladas foram superiores à maioria desses estudos anteriores.…”

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Uso de coquetéis lignocelulolíticos produzidos por consórcio fúngico na sacarificação de bagaço de cana-de-açúcar e produção de etanol

Rodrigues¹

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The present study evaluated the production of lignocellulolytic cocktails by solid-state fermentation (SSF), using sugarcane bagasse and wheat bran as substrates and the fungal strains Aspergillus niger SCBM1 (Ni), Aspergillus fumigatus SCBM6 (Fu), Ganoderma lucidum 601 (Ga), Trametes versicolor 561 (Tr) and Pleurotus ostreatus PLO6 (Pl) in monocultures and/or in consortia. The crude extracts produced were evaluated in the hydrolysis of sugarcane bagasse pretreated by autohydrolysis (AH) and by autohydrolysis followed by alkaline pretreatment (NaOH) (AH-Soda). The enzymatic cocktails that presented the best yields in terms of fermentable sugars were selected and evaluated in new hydrolyses experiments with high loads of solids and with addition of the surfactant Triton X-100. At last, alcoholic fermentations of the hydrolysates containing the highest glucose concentrations were carried out. In the SSF performed with isolated strains, the fermentation that used A. niger SCBM1 as inoculum (Ni-F1 extract) was the most outstanding in the production of enzymes, with maximum activities for endoglicanae (82.70 U/g), exoglicanae (80.48 U/g), βxylosidase (145.01 U/g) and manganese peroxidase (3.38 U/g). For the SSF performed in co-culture of fungal strains, the extracts produced by consortia between Ni+Fu+Pl (F9), Ni+Tr+Pl (F6) and Ni+Ga+Pl (F10) presented the highest productions of cellulases and hemicellulases, mainly β-glucosidase (104.70, 171.09 and 106.10 U/g), β-xylosidase (89.72, 139.99 and 121.02 U/g) and xylanase (2582.38, 2267.96 and 1697.74 U/g), respectively. The crude cocktail produced from the consortium between Basidiomycetes (Tr+Ga+Pl) was the most efficient for laccase production (25.27 U/g). After the hydrolyses, the highest glucose concentrations were obtained in the hydrolysates of sugarcane bagasse pretreated by AH and AH-Soda using Ni extract (F1) with 10.78 and 10,32 g/L, Fu+Ga+Pl extract (F11) with 9.76 g/L (AH) and 10.60 g/L (AH-Soda) and Ni+Ga+Pl extract (F10) with 11.92 g/L in AH-Soda hydrolysate. For xylose, the highest release was obtained after F8 hydrolysis that used Ni+Fu+Tr extract in sugarcane bagasse pretreatment by AH (5.08 g/L). After alcoholic fermentation, 60.80 % (in relation to the theoretical maximum) of conversion was achieved in F1 (Ni hydrolysate), with a production of 3,20 g/L of ethanol and 58,15 % in F6 (3,08 g/L) of ethanol. In hydrolysis performed with high load of solids, the results obtained showed that the concentrations of total reducing sugars (TRS) increased from 15.54 to 44.84 g/L in Ni hydrolysate (F1), when the percentage of bagasse pretreatment by AH increased from 10 to 35 %. In the Ni+Tr+Pl (F6) hydrolysate, TRS concentration increased from 11.38 to 31.77 g/L under the same conditions. The addition of surfactant did not significantly influenced concentration of TRS in Ni hydrolysate (45.16 g/L). In the F6 hydrolysis, the TRS concentration increased from 30.94 to 48.16 g/L, suggesting that for this extract, the use of surfactant favored the enzymatic action. The...

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Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation

Cited by 40 publications

References 31 publications

Use of wastes from the tea and coffee industries for the production of cellulases using fungi isolated from the Western Ghats of India

Use of wastes from the tea and coffee industries for the production of cellulases using fungi isolated from the Western Ghats of India

Understanding the effect of severity factor of prehydrolysis on dissolving pulp production using prehydrolysis kraft pulping and elemental chlorine-free bleaching sequence

Uso de coquetéis lignocelulolíticos produzidos por consórcio fúngico na sacarificação de bagaço de cana-de-açúcar e produção de etanol

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