Utilization of a mouse/human chimeric model for long term metabolic testing of human skin

Quantin, Paul; Ghazi, K.; Pereira, Ulysse; Smith, Avi; Degardin, L.; Vigneron, Pascale; Ficheux, H.; Garlick, Jonathan A.; Rapetti, Laetitia; Egles, Christophe

doi:10.1016/j.vascn.2019.106663

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Each of the 91 mouse models was used for one of the following applications, as shown in Table 2: Physiology and pharmacology of the skin (25 models); immunology, graft rejection and tolerance (17 models); carcinogenesis and skin cancer (9 models); skin diseases (25 studies), and wound healing and scars (15 models). Physiology and Pharmacology of the Skin (25 Models) [Krueger et al, 1983;Petersen et al, 1984;Reifenrath et al, 1984;Gross et al, 1985;van Genderen et al, 1985;Robertson et al, 1986;McGown et al, 1987;Klain, 1989;Kanitakis et al, 1990;Kaufmann et al, 1993;Yan et al, 1993;Otulakowski et al, 1994;Petzelbauer et al, 1996;Young et al, 1996;Rosenblatt-Velin et al, 1997;White et al, 1999;Carballido et al, 2003;Moscicka et al, 2003;Kappes et al, 2004;Capt et al, 2007;Kang and Shen, 2012;Staunstrup et al, 2013;Makino et al, 2014;Soria et al, 2014;Quantin et al, 2020] These models were used to study specific cellular types (such as Langerhans' cells), skin metabolism and molecu-lar biology, and to study macroscopical and histological changes in human skin associated with the effect of physical and chemical agents (such as ciclosporine or ultraviolet irradiation).…”

Section: Resultsmentioning

confidence: 99%

Mouse Models for Human Skin Transplantation: A Systematic Review

Cristóbal

Asúnsolo

Sánchez

et al. 2021

Cells Tissues Organs

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Immunodeficient mouse models with human skin xenografts have been developed in the past decades to study different conditions of the skin. Features such as follow-up period and size of the graft are of different relevance depending on the purpose of an investigation. The aim of this study is to analyze the different mouse models grafted with human skin. A systematic review of the literature was performed in line with the PRISMA statement using MEDLINE/PubMed databases from January 1970 to June 2020. Articles describing human skin grafted onto mice were included. Animal models other than mice, skin substitutes, bioengineered skin, postmortem or fetal skin, and duplicated studies were excluded. The mouse strain, origin of human skin, graft dimensions, follow-up of the skin graft, and goals of the study were analyzed. Ninety-one models were included in the final review. Five different applications were found: physiology of the skin (25 models, mean human skin graft size 1.43 cm2 and follow-up 72.92 days), immunology and graft rejection (17 models, mean human skin graft size 1.34 cm2 and follow-up 86 days), carcinogenesis (9 models, mean human skin graft size 1.98 cm2 and follow-up 253 days), skin diseases (25 models, mean human skin graft size 1.55 cm2 and follow-up 86.48 days), and would healing/scars (15 models, mean human skin graft size 2.54 cm2 and follow-up 129 days). The follow-up period was longer in carcinogenesis models (253 ± 233.73 days), and the skin graft size was bigger in wound healing applications (2.54 ± 3.08 cm2). Depending on the research application, different models are suggested. Careful consideration regarding graft size, follow-up, immunosuppression, and costs should be analyzed and compared before choosing any of these mouse models. To our knowledge, this is the first systematic review of mouse models with human skin transplantation.

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Section: Resultsmentioning

confidence: 99%

Mouse Models for Human Skin Transplantation: A Systematic Review

Cristóbal

Asúnsolo

Sánchez

et al. 2021

Cells Tissues Organs

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“…Foreskin grafting is the most common in human-to-mouse skin transplantation experiment, combining high regeneration and good availability [ 29 , 31 , 42 , 52 , 53 ]. Adult skin is appropriate to study normal skin physiology, regeneration, and disease modelling [ 47 , 54 , 55 ]. For several skin pathologies, a skin lesion biopsy can be used for transplantation [ 56 , 57 ].…”

Section: Basic Principles In Xenotransplantationmentioning

confidence: 99%

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

Cherkashina,

Morgun,

Rippa

et al. 2023

IJMS

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Most of the knowledge about human skin homeostasis, development, wound healing, and diseases has been accumulated from human skin biopsy analysis by transferring from animal models and using different culture systems. Human-to-mouse xenografting is one of the fundamental approaches that allows the skin to be studied in vivo and evaluate the ongoing physiological processes in real time. Humanized animals permit the actual techniques for tracing cell fate, clonal analysis, genetic modifications, and drug discovery that could never be employed in humans. This review recapitulates the novel facts about mouse skin self-renewing, regeneration, and pathology, raises issues regarding the gaps in our understanding of the same options in human skin, and postulates the challenges for human skin xenografting.

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Dermatokinetics: Advances and Experimental Models, Focus on Skin Metabolism

Quantin

Stricher

Catoire³

et al. 2022

CDM

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Numerous dermal contact products, such as drugs or cosmetics, are applied on the skin, the first protective barrier to their entrance into the organism. These products contain various xenobiotic molecules that can penetrate the viable epidermis. Many studies have shown that keratinocyte metabolism could affect their behavior by biotransformation. While aiming for detoxification, toxic metabolites can be produced. These metabolites may react with biological macromolecules often leading to sensitization reactions. After passing through the epidermis, xenobiotics can reach the vascularized dermis and therefore be bioavailable and distributed into the entire organism. To highlight these mechanisms, dermatokinetics, based on the concept of pharmacokinetics, has been developed recently. It provides information on the action of xenobiotics that penetrate the organism through the dermal route. The purpose of this review is first to describe and synthesize the dermatokinetics mechanisms to consider when assessing the absorption of a xenobiotic through the skin. We focus on skin absorption and specifically on skin metabolism, the two main processes involved in dermatokinetics. In addition, experimental models and methods to assess dermatokinetics are described and discussed to select the most relevant method when evaluating, in a specific context, dermatokinetics parameters of a xenobiotic. We also discuss the limits of this approach as it is notably used for risk assessment in the industry where scenario studies generally focus only on one xenobiotic and do not consider interactions with the rest of the exposome. The hypothesis of adverse effects due to the combination of chemical substances in contact with individuals and not to a single molecule are being increasingly studied and embraced in the scientific community.

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Utilization of a mouse/human chimeric model for long term metabolic testing of human skin

Cited by 3 publications

References 31 publications

Mouse Models for Human Skin Transplantation: A Systematic Review

Mouse Models for Human Skin Transplantation: A Systematic Review

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

Dermatokinetics: Advances and Experimental Models, Focus on Skin Metabolism

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