Utilização do programa Cells Analyser® no estudo comparativo entre imagens do endotélio de córneas obtidas por microscopia especular

Melo, Cinthia Mendonça de; Santos, Procópio Miguel dos; Santos, Rosa Maria Nascimento dos; Abib, Fernando César

doi:10.1590/s0004-27492008000100017

Cited by 5 publications

(2 citation statements)

References 14 publications

(12 reference statements)

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“…As instructed by the user manual, we identified the centers of on average 15 (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) cells per image. The software in this model measures the distances between individual cell centers and then computes the mean endothelial cell area and hence the endothelial cell density (15) .…”

Section: Discussionmentioning

confidence: 99%

“…In this study the COV was close in both uveitis groups, it can be reliably used to generate useful data on cell area and cell density, however it should be used cautiously for polymegathism estimations (16) . A recent Brazilian study related the importance of the Cells Analyzer ® software for corneal specular microscopy; its using produced representative, standardized and reproducible results in normal endothelium (20) ; however such software should not be applicable in our study due to endothelial abnormalities presented in our sample.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Corneal specular microscopy in infectious and noninfectious uveitis

Oliveira¹,

Motta²,

Muccioli³

2009

Arq. Bras. Oftalmol.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Corneal specular microscopy in infectious and noninfectious uveitis

Oliveira¹,

Motta²,

Muccioli³

2009

Arq. Bras. Oftalmol.

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Further Analysis of the Predictability of Corneal Endothelial Cell Density Estimates When Polymegethism Is Present

Doughty¹

2017

Cornea

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Possible Sampling Error in Corneal Specular Microscopy: Can Central Endothelial Data Generated by Specular Microscope Represent the Real Clinical Condition of the Whole Cornea?

Berg

Holzchuh

Hida

2020

Cornea

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Purpose: To estimate the minimum number of cells required to obtain reliable data in a specular microscope, which could possibly represent the real clinical condition of the corneal endothelium. Methods: A cross-sectional study of 122 eyes of 61 individuals submitted to noncontact specular microscope was conducted. Data generated by the manufacturer's software were uploaded to specific statistical software for sampling relative error calculation. When relative error was above 5%, new images were acquired and more cells counted until the desired relative error was reached. Data analyzed in this study for the desired relative error were number of cells marked by the examiner for each eye (marked cells), number of cells used for data analysis (analyzed cells), endothelial area used for analysis, sampling error, and absolute number of images used for each eye. Results: The average number of marked cells required to obtain a relative sampling error of less than 5% was 425.2 ± 102.2 cells. The average number of analyzed cells used by the specular microscope to generate the data was 247.4 ± 51.6 cells. The average endothelial area of the analyzed cells was 0.43 ± 0.08 mm2. The mean sampling error was 3.7% ± 0.6%, and an average of 2.95 ± 0.74 images was needed to obtain a relative sampling error of less than 5%. Conclusions: We conclude that, theoretically, a minimum of 425.2 cells from 2.95 images must be marked to obtain reliable results, which could possibly represent the real endothelial clinical condition of the whole cornea.

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Utilização do programa Cells Analyser® no estudo comparativo entre imagens do endotélio de córneas obtidas por microscopia especular

Cited by 5 publications

References 14 publications

Corneal specular microscopy in infectious and noninfectious uveitis

Corneal specular microscopy in infectious and noninfectious uveitis

Further Analysis of the Predictability of Corneal Endothelial Cell Density Estimates When Polymegethism Is Present

Possible Sampling Error in Corneal Specular Microscopy: Can Central Endothelial Data Generated by Specular Microscope Represent the Real Clinical Condition of the Whole Cornea?

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