Utility of spa typing for investigating the local epidemiology of MRSA on a UK intensive care ward

Khandavilli, Suneeta; Wilson, Peter; Cookson, Barry; Cepeda, Jorge; Bellingan, Geoffrey; Brown, Jeremy S.

doi:10.1016/j.jhin.2008.09.011

Cited by 26 publications

(24 citation statements)

References 24 publications

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“…Spa typing assigned these strains to t018, a spa type previously found in common methicillin-resistant S. aureus clones in the United Kingdom and Denmark (Bartels et al, 2009;Khandavilli et al, 2009), as well as S. aureus strains associated with nasal colonization, infections, and food poisoning (Wattinger et al, 2012). These seven highly similar isolates originated from food samples, nasal swabs of the caterer, as well as a stool sample from a patient.…”

Section: Discussionmentioning

confidence: 93%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

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In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy. Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transform-infrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determine the presence/absence of genes involved in virulence and antimicrobial resistance. We were able to match an enterotoxigenic strain from the stool sample of a patient to isolates of the same strain obtained from food and the nasal cavity of a food handler. The strain produced the enterotoxin SEA and the toxic shock syndrome toxin-1, and was also found to exhibit the genes encoding enterotoxins SEG and SEI, as well as the enterotoxin gene cluster egc. This is one of only a few studies that were able to link a staphylococcal food poisoning outbreak to its source. In 2008, 150 people gathered for a wedding celebration in Baden-Wü rttemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy.Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transforminfrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determin...

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Section: Discussionmentioning

confidence: 93%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

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“…Spa types t008, t015, t056, and t084 were reported among methicillinsensitive S. aureus causing infections in humans [22,23] and spa type t018 was found in common MRSA clones in the United Kingdom and Denmark [24,25].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections

Wattinger

Stephan

Layer

et al. 2011

Eur J Clin Microbiol Infect Dis

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Staphylococcus aureus represents an organism of striking versatility. While asymptomatic nasal colonization is widespread, it can also cause serious infections, toxinoses and life-threatening illnesses in humans and animals. Staphylococcal food poisoning (SFP), one of the most prevalent causes of foodborne intoxication worldwide, results from oral intake of staphylococcal enterotoxins leading to violent vomiting, diarrhea and cramps shortly upon ingestion. The aim of the present study was to compare isolates associated with SFP to isolates collected from cases of human nasal colonization and clinical infections in order to investigate the role of S. aureus colonizing and infecting humans as a possible source of SFP. Spa typing and DNA microarray profiling were used to characterize a total of 120 isolates, comprising 50 isolates collected from the anterior nares of healthy donors, 50 isolates obtained from cases of clinical infections in humans and 20 isolates related to outbreaks of staphylococcal food poisoning. Several common spa types were found among isolates of all three sources (t015, t018, t056, t084). DNA microarray results showed highly similar virulence gene profiles for isolates from all tested sources. These results suggest contamination of foodstuff with S. aureus colonizing and infecting food handlers to represent a source of SFP.

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“…In Ireland, ST22-MRSA-IV isolates belong to a PFGE group described as pulsed-field group 01 (PFG-01) and exhibit the non-multiantibiotic-resistant antibiogram-resistogram (AR) type AR06 or closely related AR patterns (47). ST22-MRSA-IV isolates also yield a limited number of spa types following DNA sequencing of the protein A (spa) gene (27,55).…”

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confidence: 99%

Enhanced Discrimination of Highly Clonal ST22-Methicillin-Resistant Staphylococcus aureus IV Isolates Achieved by Combining spa , dru , and Pulsed-Field Gel Electrophoresis Typing Data

et al. 2010

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Utility of spa typing for investigating the local epidemiology of MRSA on a UK intensive care ward

Cited by 26 publications

References 24 publications

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections

Enhanced Discrimination of Highly Clonal ST22-Methicillin-Resistant Staphylococcus aureus IV Isolates Achieved by Combining spa , dru , and Pulsed-Field Gel Electrophoresis Typing Data

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