Utility of <scp>MGG</scp> and Papanicolaou stained smears in the detection of Mucormycosis in nasal swab/scraping/biopsy samples of <scp>COVID</scp> 19 patients

Philip, Ashish C.; P, Madan; Sharma, Sonal; Das, Shukla

doi:10.1002/dc.24924

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…Philip et al conducted a study in a tertiary health care hospital. 37 Thirty‐two nasal swabs/scrapings/biopsy samples from patients suspected to have mucormycosis were subjected to KOH testing. Sixteen of the cases were positive while 16 were negative on KOH preparation.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of liquid‐based cytology in the diagnosis of mucormycosis in COVID‐19 treated patients

et al. 2022

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Background The coronavirus disease 2019 (COVID‐19) infection caused by the novel severe acute respiratory syndrome corona virus 2 (SARS‐CoV‐2) is associated with a wide range of disease patterns, ranging from mild to life‐threatening pneumonia. COVID‐19 can be associated with a suppressed immune response and/or hyperinflammatory state due to a cytokine storm. Reduced immunity, combined with steroid usage to prevent a cytokine storm along with various pre‐existing comorbidities can prove to be fertile ground for various secondary bacterial and fungal infection, including mucormycosis. Diagnosis of Mucor is a challenging task given the high negativity rate of various detection methods. While histopathology is considered the gold standard, the acquisition of necessary tissue biopsy specimens requires invasive procedures and is time consuming. Method In this study five different methods of Mucor detection, namely conventional cytopathology, liquid‐based cytology (LBC, BD SurePath™), potassium hydroxide (KOH) preparation, culture, and histopathology were analysed. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for all five methods. Results LBC had values for sensitivity, specificity, PPV, and NPV of 72.4%, 100%, 100%, and 38.4%, respectively, closely matching histopathology in sensitivity (75.9%). The sensitivity of culture, conventional cytopathology, and KOH were very low compared to histopathology and LBC. Conclusion This study showed that LBC, can be a rapid and effective alternative to histopathology in Mucor diagnosis.

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Section: Discussionmentioning

confidence: 99%

Sensitivity of liquid‐based cytology in the diagnosis of mucormycosis in COVID‐19 treated patients

et al. 2022

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Utility of MGG and Papanicolaou stained smears in the detection of Mucormycosis in nasal swab/scraping/biopsy samples of COVID 19 patients

Philip

Sharma

et al. 2021

Diagnostic Cytopathology

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Background COVID 19 has been rapidly spreading across the globe. As a result of alteration of the immune milieu by COVID 19 and its treatment, there has been a rise in opportunistic fungal infections particularly Mucormycosis in these patients. Delay in diagnosis of these fungal infections can be fatal. The usual diagnostic modalities used to detect Mucor include potassium hydroxide (KOH) mount, fungal culture, and histopathology. Since histopathology and fungal culture have a long turnaround time we are dependent on KOH mount for rapid results. Here we investigate the role of stained cytology smears in the rapid diagnosis of Mucormycosis. Methods A prospective observational study was conducted in a tertiary health care hospital on samples of patients clinically suspected to have Mucormycosis. We performed May Grunwald Giemsa (MGG) and Papanicolaou (PAP) stains on the remnant samples of nasal swabs/scrapings/biopsies after KOH test and fungal culture. We took 16 KOH positive and 16 KOH negative samples. We also examined 16 fresh samples from patients whose earlier samples were reported to be negative on KOH test. Results The 6/16 KOH positive samples were found to be positive on stained cytology smears and 2 were mixed infections wherein both Mucor and Aspergillus were seen. The 4/16 KOH negative samples were positive for Mucor with one sample having both Mucor and Aspergillus. The 3/16 repeat samples which were earlier negative on KOH test were positive for Mucor. Conclusion Stained cytology smears if used in conjunction with KOH test can increase the overall sensitivity of detection of Mucormycosis and mixed infections.

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Severe mold fungal infections in critically ill patients with COVID-19

Koulenti,

Paramythiotou,

Almyroudi

et al. 2024

Future Microbiology

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Utility of MGG and Papanicolaou stained smears in the detection of Mucormycosis in nasal swab/scraping/biopsy samples of COVID 19 patients

Cited by 4 publications

References 26 publications

Sensitivity of liquid‐based cytology in the diagnosis of mucormycosis in COVID‐19 treated patients

Sensitivity of liquid‐based cytology in the diagnosis of mucormycosis in COVID‐19 treated patients

Utility of MGG and Papanicolaou stained smears in the detection of Mucormycosis in nasal swab/scraping/biopsy samples of COVID 19 patients

Severe mold fungal infections in critically ill patients with COVID-19

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