Utility of NBD-Cl for the spectrophotometric determination of some skeletal muscle relaxant and antihistaminic drugs

Saleh, Hanaa; Elhenawee, Magda; Ragab, Gamal H.; El-Hay, Soad S. Abd

doi:10.1016/j.saa.2006.09.039

Cited by 43 publications

(20 citation statements)

References 40 publications

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“…It has also been used in charge transfer reactions due to its electrophilic properties, where it acts as -acceptor for determination of some β-blockers [54], some skeletal muscle relaxant and antihistaminic drugs [55].…”

Section: Resultsmentioning

confidence: 99%

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

et al. 2013

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KEYWORDSSeveral simple, sensitive, accurate and inexpensive spectrophotometric and spectrofluorimetric methods were developed for the determination of propafenone HCl and diltiazem HCl using 4-chloro-7-nitrobenzofurazan (NBD-Cl) accompanied with kinetic study, either in pure form or in pharmaceutical preparations. In this work, the cited drugs react with (NBD-Cl) in presence of borate buffer of pH = 7.6 at a fixed time of 30 minutes on thermostated water bath at (75-80 °C). The absorbance was measured using spectrophotometric technique at 489 and 481 nm for propafenone HCl and diltiazem HCl, respectively, or by using spectrofluorimetric technique after dilution at the specific wavelength of excitation and emission.The calibration curves were linear in the range of 4-44, 16-96 µg/mL when using spectrophotometric method, and 0.4-3.6, 1.6-8.8 µg/mL when spectrofluorimetric method was applied for propafenone HCl and diltiazem HCl, respectively. The limit of quantitation and the limit of detection were also calculated. The methods were applied successfully to commercial dosage form and can be further applied for their determination on a large scale in quality control laboratories. The obtained results statistically agreed with those obtained by reference methods. The determination of the studied drugs by the fixed concentration and rate constant methods is feasible with the calibration equations obtained, but the fixed time method proves to be more applicable.Diltiazem HCl Propafenone HCl Spectroflourimetric Spectrophotometric Kinetic determination 4-Chloro-7-nitrobenzofurazan

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Section: Resultsmentioning

confidence: 99%

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

et al. 2013

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“…NBD has been used as a fluorogenic or chromogenic reagent in pharmaceutical analysis. The analysis of different compounds/drugs either in pharmaceuticals/biological samples was performed after derivatization with NBD followed by measuring the resulted product by means of spectrophotometry [33][34][35][36][37][38] and spectrofluorimetry [39,40]. Due to electrophilic properties, NBD has also been used in charge transfer reactions.…”

Section: Construction Of Calibration Curve: For Both the Proposedmentioning

confidence: 99%

“…Due to electrophilic properties, NBD has also been used in charge transfer reactions. This property is used in the quantification of various β-blockers [41], skeletal muscle relaxant [33] and antihistaminic drugs [33].…”

Section: Construction Of Calibration Curve: For Both the Proposedmentioning

confidence: 99%

Spectrophotometric quantification of direct factor xa inhibitor, rivaroxaban, in raw and tablet dosage form

Bsv¹,

Cb²

2017

Glob Drugs Therap

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“…Many efforts have been devoted to the development of analytical methods for the determination of BAL using spectrophotometry [3,4], potentiometry [5], thin-layer chromatography [6], gas chromatography [7], high-performance liquid chromatography (HPLC) [8,9,10] and capillary electrophoresis [11,12]. As the target analyte is usually present in complex samples at low concentrations, HPLC coupled with all kinds of detection methods such as UV-vis absorption [8,9], fluorescence detection [10] and mass spectrometry (MS) [13] is an especially effective and important technique due to its separation process and high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cao

2012

Acta Chromatographica

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Summary. Two rapid, sensitive and reproducible methods for the determination of baclofen(BAL) in urine and plasma based on high-performance liquid chromatography (HPLC) with UV-vis and fluorescent detection, respectively, were developed for the first time using a new synthesized fluorescent label, 6-oxy-(N-succinimidylacetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF). The optimal derivatization yield was achieved in borate buffer (pH 8.0) for 15 min at room temperature (25 °C). With a mixture of methanol and water containing 5 mmol L −1 sodium citrate buffer (pH 5.0) as mobile phase, BAL was determined at λ = 455 nm with UV-vis detection and at λ ex /λ em = 488/520 nm with FD detection. The detection limits are 1.065 × 10 −3 mg mL −1 and 1.065 × 10 −2 mg mL −1 with HPLC-UV-vis and HPLC-FD, respectively. The proposed method has been successfully applied to the analysis of BAL in human urine and plasma samples. The established method is rapid (15 min of derivatization process and 10 min of chromatographic run), reproducible and sensitive.

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Utility of NBD-Cl for the spectrophotometric determination of some skeletal muscle relaxant and antihistaminic drugs

Cited by 43 publications

References 40 publications

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

Spectrophotometric quantification of direct factor xa inhibitor, rivaroxaban, in raw and tablet dosage form

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

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