Utility of fluorescamine-based approach for highly sensitive spectrofluorimetric determination of Ceftazidime and Vancomycin in pharmaceuticals and real human plasma

Marzouq, Mostafa A.; Salman, Baher I.; Hussein, S. A.; Ali, Marwa F.

doi:10.1016/j.microc.2018.10.037

Cited by 23 publications

(23 citation statements)

References 15 publications

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“…Each 0.5 ml of plasma was transferred to a centrifugation tube and spiked with various aliquots of the standard SPRC solution. Here, 1 ml acetonitrile was utilized as a protein precipitation agent, [17][18][19][20] the content was completed to 10 ml with distilled water and then centrifuged at 18 659 g for 10 min. The general analytical procedure was performed exactly as described in Section 2.4.…”

Section: Procedures For Detection Of Spec In Human Plasmamentioning

confidence: 99%

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Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

et al. 2019

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Spectinomycin hydrochloride (SPEC) is an aminoglycoside antibiotic that has a broad spectrum against several bacterial strains of humans and veterinary infections.However, SPEC lacks a fluorophore or chromophore and this lack makes its analysis a challenge. Our study provides a simple, sensitive and low-cost spectrofluorimetric/spectrophotometric method based on the reaction between secondary amine groups and a benzofurazan reagent using borate buffer (pH 9.2). The yielding compound was measured fluorimetrically at 530 nm (excitation at 460 nm) with colorimetry at 410 nm. The calibration curves ranged from 30 to 400 ng ml −1 and from 0.2 to 6 μg ml −1 for spectrofluorimetric and spectrophotometric analyses, respectively. The limits of detection were calculated to be 4.15 ng ml −1 and 0.05 μg ml −1 for spectrofluorimetric and spectrophotometric processes, respectively.The ultra-affectability and high selectivity of the proposed method permitted analysis of SPEC in the dosage form and in human plasma samples, with good recoveries of about 101.19 and 97.11%, respectively, without any matrix interference. The proposed strategy was validated using International Conference on Harmonization standards and validated bioanalytically using USFDA recommendations.

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Section: Procedures For Detection Of Spec In Human Plasmamentioning

confidence: 99%

“…Stoichiometry between SPEC and NBD-Cl was checked using a continuous variation method (Job's method), [22] as described previously [20,23,24] by preparing 1 × 10 −4 M SPEC and NBD-Cl.…”

Section: Stoichiometry Mechanismmentioning

confidence: 99%

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

et al. 2019

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“…The amount of Pheo a conjugated to γ-PGA was determined by measuring the absorbance at 405 nm using an ultraviolet–visible (UV–visible) spectrometer. The amount of the targeting ligand EAE7 decorated onto HA was also measured using a fluorescamine assay [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

Photodynamic and Cold Atmospheric Plasma Combination Therapy Using Polymeric Nanoparticles for the Synergistic Treatment of Cervical Cancer

Kim

2021

IJMS

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Integrating multi-modal therapies into one platform could show great promise in overcoming the drawbacks of conventional single-modal therapy and achieving improved therapeutic efficacy in cancer. In this study, we prepared pheophorbide a (Pheo a)/targeting ligand (epitope analog of oncoprotein E7, EAE7)-conjugated poly(γ-glutamic acid) (γ-PGA)/poly(lactide-co-glycolide)-block-poly(ethylene glycol) methyl ether (MPEG-PLGA)/hyaluronic acid (PPHE) polymeric nanoparticles via self-assembly and encapsulation method for the photodynamic therapy (PDT)/cold atmospheric plasma (CAP) combinatory treatment of human papillomavirus (HPV)-positive cervical cancer, thereby enhancing the therapeutic efficacy. The synthesized PPHE polymeric nanoparticles exhibited a quasi-spherical shape with an average diameter of 80.5 ± 17.6 nm in an aqueous solution. The results from the in vitro PDT efficacy assays demonstrated that PPHE has a superior PDT activity on CaSki cells due to the enhanced targeting ability. In addition, the PDT/CAP combinatory treatment more effectively inhibited the growth of cervical cancer cells by causing elevated intracellular reactive oxygen species generation and apoptotic cell death. Moreover, the three-dimensional cell culture model clearly confirmed the synergistic therapeutic efficacy of the PDT and the CAP combination therapy using PPHE on CaSki cells. Overall, these results indicate that the PDT/CAP combinatory treatment using PPHE is a highly effective new therapeutic modality for cervical cancer.

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“…According to the literature survey, some techniques were reported for the estimation of VANH in different matrices including spectrophotometric [4][5][6], spectro uorimetric [7][8][9], electrochemical [10][11][12], HPLC [13][14][15][16][17][18][19][20][21], LC-MS/MS [22][23][24][25][26][27][28][29][30][31][32][33], capillary electrophoresis [34][35][36][37], radioimmunoassay and ELISA-based immunoassay techniques [38][39][40]. VANH has native uorescence at 335 nm after excitation at 268 nm according to this study [8].…”

Section: Introductionmentioning

confidence: 99%

Utility of silver-nanoparticles for nano-fluorimetric determination of vancomycin hydrochloride in pharmaceutical formulation and biological fluids: Greenness assessment

Mohamed

2022

Preprint

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Vancomycin hydrochloride (VANH) is a glycopeptide antibiotic commonly employed in the prophylaxis and therapy of various gram-positive bacterial life-threatening infections. Due to the narrow therapeutic window of VANH, its serum levels should be well-monitored to avoid toxicity and optimize its therapy. Herein, an innovative silver-nanoparticles enhanced fluorescence technique was designed for VANH rapid analysis in miscellaneous matrices. This technique is based on reinforcement of VANH fluorescence intensity with silver-nanoparticles that were synthesized by a redox reaction between VANH and silver nitrate in NaOH alkaline medium using polyvinylpyrrolidone as a stabilizer. The produced silver-nanoparticles were characterized by using UV-visible spectroscopy and transmission electron microscope (TEM) micrograph. The fluorescence intensity was measured at 394 nm after excitation at 259 nm. Under optimum conditions, a good linear relationship was accomplished between the VANH concentration and the fluorescence intensity in a range of (1-36) ng/mL with a limit of detection of 0.29 ng/mL. Greenness assessment was performed using two assessment tools namely; eco-scale scoring and green analytical procedure index revealing excellent greenness of the proposed technique. The proposed technique was validated according to ICH recommendations and statistically compared with the reported HPLC method revealing no significant difference concerning accuracy and precision at p=0.05. The proposed technique depended primarily on the water as a cheap and eco-friendly solvent consequently; it’s regarded as a green alternative to the expensive chromatographic techniques that utilize hazardous organic solvents. Furthermore, the attained ultrasensitivity of the proposed technique can be adapted for VANH monitoring and studying its bioequivalence in biological fluids.

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Utility of fluorescamine-based approach for highly sensitive spectrofluorimetric determination of Ceftazidime and Vancomycin in pharmaceuticals and real human plasma

Cited by 23 publications

References 15 publications

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

Photodynamic and Cold Atmospheric Plasma Combination Therapy Using Polymeric Nanoparticles for the Synergistic Treatment of Cervical Cancer

Utility of silver-nanoparticles for nano-fluorimetric determination of vancomycin hydrochloride in pharmaceutical formulation and biological fluids: Greenness assessment

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