Utility of eosin Y as a complexing reagent for the determination of citalopram hydrobromide in commercial dosage forms by fluorescence spectrophotometry

Abstract

An accurate, selective and sensitive spectrofluorimetric method was developed for the determination of citalopram hydrobromide in commercial dosage forms. The method was based on the formation of a fluorescent ion-pair complex between citalopram hydrobromide and eosin Y in the presence of a disodium hydrogen phosphate/citric acid buffer solution of pH 3.4 that was extractable in dichloromethane. The extracted complex showed fluorescence intensity at λem = 554 nm after excitation at 259 nm. The calibration curve was linear over at concentrations of 2.0-26.0 µg/mL. Under optimized experimental conditions, the proposed method was validated as per ICH guidelines. The effect of common excipients used as additives was tested and the tolerance limit calculated. The limit of detection for the proposed method was 0.121 μg/mL. The proposed method was successfully applied to the determination of citalopram hydrobromide in commercial dosage forms. The results were compared with the reference RP-HPLC method.

Validated Analytical Spectrofluorimetric Method for Quantitation of Diphenhydramine HCl in Commercial Dosage Forms

“…In the literature, it was reported that chlorpheniramine [14], doxepin HCl [15], citalopram HBr [16] and imipramine [17] of the diphenhydramine and -C=O of eosin Y. The reaction sequence is shown in Fig.…”

Application of a xanthene dye, eosin Y, as spectroscopic probe in chemical and pharmaceutical analysis; a review

“…Extractive spectrofluorimetric methods were reported for some drugs based on either binary or ternary complex formation with eosin Y. Binary complex formation was applied for the determination of citalopram hydrobromide (Azmi et al 2015) and doxepin hydrochloride (Rahman, Siddiqui & Azmi, 2009) in commercial dosage forms. The method was based on the formation of an ion-pair complex between the drug and eosin Y in the presence of acetate buffer (at pH 3.4 for citalopram and pH 4.52 for doxepin).…”

Utilization of Eosin Dye as an Ion Pairing Agent for Determination of Pharmaceuticals: A Brief Review

“…Moreover, the high sensitivity and specificity offered by this method are also higher and the drug compounds can be analyzed up to nano levels. As data are shown in table 1, these methods were widely used in quantitation of many pharmaceuticals compounds using eosin Y due to its simplicity, low cost, high sensitivity and wide concentration range [52][53][54][55][56][57][58][59][60][61][62][63][64][65][66][67][68].…”