Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma

Ikeda, Chisaki; Haga, Hiroaki; Makino, Naohiko; Inuzuka, Tatsutoshi; Kurimoto, Ayako; Ueda, Toshiki; Matsuda, Akiko; Kakizaki, Yasuharu; Ishizawa, Tetsuya; Kobayashi, Toshikatsu; Sugahara, Shinpei; Tsunoda, Michihiko; Suda, Kensei; Ueno, Yoshiyuki

doi:10.1038/s41598-021-81023-y

Cited by 18 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to non-coding RNA, proteins in extracellular vesicles can also be used as markers for patients with CCA. Ikeda, C et al (121)used EDEG to separate human bile EVs and analyzed proteins in extracellular vesicles for the first time. They found that Claudin-3 in bile EVs can distinguish between CCA and patients with bile duct stones with a sensitivity and specificity of 87.5% and 87.5%, respectively (Table 3).…”

Section: Ctdnamentioning

confidence: 99%

Research progress of bile biomarkers and their immunoregulatory role in biliary tract cancers

Liu

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Biliary tract cancers (BTCs), including cholangiocarcinoma and gallbladder carcinoma, originate from the biliary epithelium and have a poor prognosis. Surgery is the only choice for cure in the early stage of disease. However, most patients are diagnosed in the advanced stage and lose the chance for surgery. Early diagnosis could significantly improve the prognosis of patients. Bile has complex components and is in direct contact with biliary tract tumors. Bile components are closely related to the occurrence and development of biliary tract tumors and may be applied as biomarkers for BTCs. Meanwhile, arising evidence has confirmed the immunoregulatory role of bile components. In this review, we aim to summarize and discuss the relationship between bile components and biliary tract cancers and their ability as biomarkers for BTCs, highlighting the role of bile components in regulating immune response, and their promising application prospects.

show abstract

Section: Ctdnamentioning

confidence: 99%

Research progress of bile biomarkers and their immunoregulatory role in biliary tract cancers

Liu

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…The expression of miR-483-5p and miR-126-3p was found to be significantly higher in bile EVs derived from patients with malignant biliary obstructions, and could distinguish benign and malignant biliary obstructions with high accuracy and specificity ( 29 ). Claudin-3, a protein derived from human bile EVs sample, was identified as cholangiocarcinoma-specific, and the diagnostic accuracy was 0.945 AUC ( 30 ). Besides, long non-coding RNAs from bile EVs, ENST00000588480.1 and ENST00000517758.1 also showed significantly up-regulated expression in patients with cholangiocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Evaluation and Application of a Novel Method to Isolate Cell-Free DNA Derived From Bile of Biliary Tract Cancer Patients

et al. 2022

View full text Add to dashboard Cite

Cell-free DNA (cfDNA) exists in various types of bodily fluids, including plasma, urine, bile, and others. Bile cfDNA could serve as a promising liquid biopsy for biliary tract cancer (BTC) patients, as bile directly contacts tumors in the biliary tract system. However, there is no commercial kit or widely acknowledged method for bile cfDNA extraction. In this study, we established a silica-membrane-based method, namely 3D-BCF, for bile cfDNA isolation, exhibiting effective recovery of DNA fragments in the spike-in assay. We then compared the 3D-BCF method with four other commercial kits: the BIOG cfDNA Easy Kit (BIOG), QIAamp DNA Mini Kit (Qiagen), MagMAXTM Cell-Free DNA Isolation Kit (Thermo Fisher), and NORGEN Urine Cell-Free Circulating DNA Purification Mini Kit (Norgen Biotek). The proposed 3D-BCF method exhibited the highest cfDNA isolation efficiency (p < 0.0001) from patient bile samples, and bile cfDNA of short, medium or long fragments could all be extracted effectively. To test whether the extracted bile cfDNA from patients carries tumor-related genomic information, we performed next-generation sequencing on the cfDNA and verified the gene-mutation results by polymerase chain reaction (PCR)-Sanger chromatograms and copy-number-variation (CNV) detection by fluorescence in situ hybridization (FISH) of tumor tissues. The 3D-BCF method could efficiently extract cfDNA from bile samples, providing technical support for bile cfDNA as a promising liquid biopsy for BTC patient diagnosis and prognosis.

show abstract

“…By using proteomics approaches, Heat shock protein 90 (HSP90) was confirmed that differentially phosphorylated in invasive CCA cells and was expected to serve as an implement for indicating the metastatic of CCA [ 69 ]. Besides, proteomic analysis showed that Claudin-3 was enriched in human bile-derived exosomes, and might become a novel biomarker for CCA as well [ 70 ]. In comparison with serum exosomes isolated from normal individuals, several specific proteins were observed to be concentrated in exosomes of CCA patients.…”

Section: Exosomes In Cca Diagnosismentioning

confidence: 99%

Exosomes in the tumor microenvironment of cholangiocarcinoma: current status and future perspectives

Zhao

Shi

et al. 2022

J Transl Med

View full text Add to dashboard Cite

Cholangiocarcinoma (CCA) refers to an aggressive malignancy with a high fatality rate and poor prognosis. Globally, the morbidity of CCA is increasing for the past few decades, which has progressed into a disease that gravely endangers human health. Exosomes belong to a class of extracellular vesicles (EVs) with diameters ranging from 40 to 150 nm that can be discharged by all living cells. As communication messengers of the intercellular network, exosomes carry a diverse range of cargoes such as proteins, nucleic acids, lipids, and metabolic substances, which are capable of conveying biological information across different cell types to mediate various physiological activities or pathological changes. Increasing studies have demonstrated that exosomes in the tumor microenvironment participate in regulating tumorigenesis and progression via multiple approaches in the tumor microenvironment. Here, we reviewed the current research progress of exosomes in the context of cancer and particularly highlighted their functions in modulating the development of CCA. Furthermore, the potential values of exosomes as diagnostic and therapeutic targets in CCA were overviewed as well.

show abstract

Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma

Cited by 18 publications

References 40 publications

Research progress of bile biomarkers and their immunoregulatory role in biliary tract cancers

Research progress of bile biomarkers and their immunoregulatory role in biliary tract cancers

Comprehensive Evaluation and Application of a Novel Method to Isolate Cell-Free DNA Derived From Bile of Biliary Tract Cancer Patients

Exosomes in the tumor microenvironment of cholangiocarcinoma: current status and future perspectives

Contact Info

Product

Resources

About