Utility of cefixime as a complexing reagent for the determination of Ni(II) in synthetic mixture and water samples

Abstract

A simple, sensitive, and accurate UV spectrophotometric method has been developed for the determination of nickel in synthetic mixture and water samples. The method is based on the complexation reaction of nickel ion with cefixime, thus leading to the formation of Ni-cefixime complex in ethanol-distilled water medium at room temperature. The complex showed the maximum absorption wavelength at 332 nm. Beer's law is obeyed in the working concentration range of 0.447-4.019 μg mL(-1) with apparent molar absorptivity of 7.314 × 10(3) L mol(-1) cm(-1) and Sandell's sensitivity of 0.008 μg/cm(2)/0.001 absorbance unit. The limits of detection and quantitation for the proposed method are 0.016 and 0.054 μg mL(-1), respectively. The factors such as cefixime concentration and solvent affecting the complexation reaction were carefully studied and optimized. The method is validated as per the International Conference on Harmonisation guideline. The method is successfully applied to the determination of Ni(II) in synthetic mixture and wadi water samples collected from Al Rustaq. The same water samples are also analyzed by atomic absorption spectrophotometry. Both methods determined the amount of Ni(II) in water sample and found to be approximately the same.

Optimization of HPLC method for determination of cefixime using 2-thiophenecarboxaldehyde as derivatizing reagent: A new approach

“…Various analytical methods have been reported for analysis of cefixime and other antibiotics after complexation and derivatization with a variety of chemical reagents (Sayed et al, 2013, El-Shaboury et al, 2007, Wani and Patil, 2013, Ahmed et al, 2011, Adegoke and Quadri, 2012, Adegoke and Umoh, 2009). These methods involve spectrophotometric methods (Thakkar and Mashru, 2012, Azmi et al, 2013, Ethiraj et al, 2012, Attimarad et al, 2012, Ramadan et al, 2013, Shah and Kilambi, 2006, Agbaba et al, 1997, El-Wailily et al, 2000), voltammetric method (Rajeev et al, 2010), capillary electrophoresis (Ahemed, 2013), clinical studies (Khan et al, 2008), flow injection spectrophotometry (Abass et al, 2011), HPLC/tandem mass spectrometry (Ronaldo et al, 2001), UPLC/HPLC & IPLC (Ion-Pairing Liquid Chromatography) (Uslu and Ozden, 2013, Manna and Valvo, 2004), alternative continuous infusion method (Hiroyuki et al, 2006) and attenuated total reflectance (ATR) fourier transform infrared (FT-IR) spectroscopy (Kandhro et al, 2013). Among these techniques, UV/Vis spectrophotometry is considered as one of the most widely used technique for determination of cefixime after derivatization (Sayed et al, 2013, El-Shaboury et al, 2007, Wani and Patil, 2013, Ahmed et al, 2011, Adegoke and Quadri, 2012, Adegoke and Umoh, 2009, Thakkar and Mashru, 2012, Azmi et al, 2013, Ethiraj et al, 2012, Attimarad et al, 2012, Ramadan et al, 2013, Shah and Kilambi, 2006, Agbaba et al, 1997, El-Wailily et al, 2000, Rajeev et al, 2010, Ahemed, 2013, Al-Momani, 2001).…”