Utility of capillary microsampling for rat pharmacokinetic studies: Comparison of tail-vein bleed to jugular vein cannula sampling

Korfmacher, Walter A.; Luo, Youfu; Ho, Stacy; Sun, Wei; Shen, Liduo; Wang, Jie; Wu, Zhongtao; Guo, Ying; Snow, Gregory; O’Shea, Thomas J.

doi:10.1016/j.vascn.2015.07.001

Cited by 17 publications

(6 citation statements)

References 10 publications

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“…Generally, rodent evaluation is the most popular form of experimentation; however, in almost all cases, the sacrifice of the animal is necessary to obtain a representative sample of heart blood. Only a few studies have reported the use of small samples of between 30 and 200 µL of serum or plasma collected by capillary microsampling from the vein cannula or tail-vein [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

et al. 2020

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The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.

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Section: Introductionmentioning

confidence: 99%

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

et al. 2020

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“…However, since there were no significant differences in any of the TK parameters (C max , T max and AUC 0-24hr ) important for assessing drug exposure between the two blood sampling sites (jugular vein and tail vein), it was concluded that there is no significant influence between sampling sites for the TK evaluation when one sampling site is used throughout the assessment period. Korfmacher et al (2015) compared the PK parameters of fluoxetine, which has a large volume of distribution (distribution volume of 20-42 L/kg) (Johnson et al, 2007), which is similar to imipramine, between jugular vein cannula sampling and capillary microsampling from the tail vein. As a result of the study, lower blood concentrations from the tail vein than from the jugular vein were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Vangala et al (2015) reported that there were no clear differences when comparing the PK parameters of dapsone (diaphenylsulfone) among the samples collected from the orbital vein, jugular vein and saphenous vein. Korfmacher et al (2015) reported that differences in the changes in blood drug concentrations of some compounds were observed in the PK study when the PK parameters of 5 compounds were compared between jugular vein cannula sampling and capillary microsampling from the tail vein. The differences in sampling site, i.e., sampling from the jugular vein, which is considered to have large blood flow, and the tail vein, which is located at the periphery, may affect the TK assessment and, in particular, compounds that may have a high volume of distribution.…”

Section: Introductionmentioning

confidence: 99%

Effects of serial cervical or tail blood sampling on toxicity and toxicokinetic evaluation in rats

Hattori

Takumi

Saito³

et al. 2020

J. Toxicol. Sci.

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To assess the influences of blood sampling volumes or sites on toxicological and toxicokinetic (TK) evaluations, 4-week duration animal studies and a single-dose TK study of imipramine were conducted. In the toxicological evaluation, six-week-old Sprague-Dawley rats were divided into no blood and blood sampling groups. Fifty microliters (microsampling) or 100 μL (larger sampling) of blood/time point was collected from the jugular vein (50 μL of data was reported previously as Yokoyama et al., 2020) or the tail vein 6 to 7 times on days 1/2 and in week 4. Although no parameters were affected by the 100 μL sample from the tail vein, the 100 μL jugular vein sampling decreased the red blood cell parameters in females, possibly due to hemorrhage at the sampling site. Regarding the TK assessment, 50 μL of blood/site/time point was collected at 6 time points from the tail and jugular vein of the same male rats after single oral administration of 10 or 100 mg/kg imipramine, which was selected as a representative drug with high distribution volume. Although there were no differences in the AUC 0-24hr and C max values between the sites, the plasma concentrations at the early time points were significantly lower from the tail vein than the jugular vein. From our studies, 50 μL of jugular and tail vein microsampling did not affect the toxicity parameters or AUC/C max . However, appropriate toxicity considerations and/or selection of the blood sampling site may be important in the case of larger sampling volumes or blood concentration assessment.

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“…It has been reported that TK parameters including the area under the concentration‐time curve (AUC) differ between the caudal and orbital venous plexus (Hui et al, 2007; Korfmacher et al, 2015), especially for drugs with relatively large partition coefficients (e.g., fluoxetine). A comparative analysis of TK parameters between blood collection sites of jugular vein and tail vein, commonly used microsampling site in Japan and Europe/United States, respectively, was performed for imipramine, which has a large volume of distribution and distribution coefficient as a model drug (Hattori et al, 2020).…”

Section: Influence Of Microsampling On the Toxicity Parameters In Rat...mentioning

confidence: 99%

Impact of microsampling on toxicological evaluation in rodent safety studies

Takahashi,

Hattori,

Yokoyama

et al. 2023

J of Applied Toxicology

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Recently, animal welfare has been attracting worldwide attention, and implementation of 3Rs (replacement, reduction, and refinement) is prioritized in every way possible in the drug development. Microsampling, in which small amounts of blood are collected, is attracting attention in this context. ICH S3A Q&A focused on microsampling was published in November 2017 to help accelerate the application of microsampling for toxicokinetic assessment. The increased sensitivity of drug measurement apparatuses such as mass spectrometers has made it possible to measure drug concentrations with small amounts of blood samples. In this review, we summarized the reports on toxicological influence of microsampling in rodents (rats and mice) with or without drug administration or recovery period after blood collection and influences that may arise from differences in the blood sampling site or blood sampling volume. We also summarized some perspectives on further implementation of microsampling in toxicology studies. The use of microsampling in regulatory toxicology studies has gradually increased, although at a lower rate than in discovery studies. Since more animals are used in GLP toxicology studies than in discovery studies, the effect of reducing the number of animals by microsampling is expected to be greater in the toxicology studies. This report aims to promote the application of microsampling to nonclinical studies, as it is beneficial for improving animal welfare and can contribute to the 3Rs.

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Utility of capillary microsampling for rat pharmacokinetic studies: Comparison of tail-vein bleed to jugular vein cannula sampling

Cited by 17 publications

References 10 publications

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

Effects of serial cervical or tail blood sampling on toxicity and toxicokinetic evaluation in rats

Impact of microsampling on toxicological evaluation in rodent safety studies

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