Utility of Amplified Fragment Length Polymorphisms (AFLP) to Analyse Genetic Structures within the Alexandrium tamarense Species Complex

John, Uwe; Groben, R.; Beszteri, Bánk; Medlin, Linda K.

doi:10.1078/143446104774199574

Cited by 51 publications

(54 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, real-time PCR and fluorescence in situ hybridization (FISH)-based assays have been designed for laboratory and field applications (21,30). New fingerprinting methods such as microsatellite, amplified fragment length polymorphism (AFLP), and intersimple sequence repeat (ISSR) markers have also been developed to analyze the genetic variability among isolates (12,32,43). In order to determine specific characteristics of locally or globally distributed Alexandrium species, a significant number of key studies are currently being carried out on their taxonomy, toxicity, life cycle, physiology, phylogeny, bloom dynamics, biogeography, and genetic diversity.…”

mentioning

confidence: 99%

Characterization of Nontoxic and Toxin-Producing Strains ofAlexandrium minutum(Dinophyceae) in Irish Coastal Waters

Touzet

Franco

Raine

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

A comparative analysis of the morphology, toxin composition, and ribosomal DNA (rDNA) sequences was performed on a suite of clonal cultures of the potentially toxic dinoflagellate Alexandrium minutum Halim. These were established from resting cysts or vegetative cells isolated from sediment and water samples taken from the south and west coasts of Ireland. Results revealed that strains were indistinguishable, both morphologically and through the sequencing of the D1-D2 domain of the large subunit and the ITS1-5.8S-ITS2 regions of the rDNA. High-performance liquid chromatography fluorescence detection analysis, however, showed that only strains derived from retentive inlets on the southern Irish coast synthesized paralytic shellfish poisoning (PSP) toxins (GTX2 and GTX3), whereas all strains of A. minutum isolated from the west coast were nontoxic. Toxin analysis of net hauls, taken when A. minutum vegetative cells were in the water column, revealed no PSP toxins in samples from Killary Harbor (western coast), whereas GTX2 and GTX3 were detected in samples from Cork Harbor (southern coast). These results confirm the identity of A. minutum as the most probable causative organism for historical occurrences of contamination of shellfish with PSP toxins in Cork Harbor. Finally, random amplification of polymorphic DNA was carried out to determine the degree of polymorphism among strains. The analysis showed that all toxic strains from Cork Harbor clustered together and that a separate cluster grouped all nontoxic strains from the western coast.

show abstract

mentioning

confidence: 99%

Characterization of Nontoxic and Toxin-Producing Strains ofAlexandrium minutum(Dinophyceae) in Irish Coastal Waters

Touzet

Franco

Raine

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…the marine dinoflagellate Alexandrium tamarense, the diatom Asterionella formosa and the chlorophyte Chlorella vulgaris, both freshwater algae. AFLP banding patterns in isolates of the dinoflagellate Alexandrium tamarense from the Orkney Islands were correlated with toxin patterns as determined by HPLC analysis (John et al, 2004), but a later study in the same area with more isolates and depending on the spatial scale investigated, AFLP patterns did not correlate with allelopathic capabilities (Alpermann 2009). A preliminary study of Phaeocystis antarctica indicated that the gyres around the Antarctic were not isolated from one another and it was likely that the ACC provided the vehicle for dispersal around the continent (Gaebler et al, 2007).…”

Section: Fingerprinting Methods As Applied To Environmental Samplesmentioning

confidence: 99%

Molecular Techniques to Estimate Biodiversity with Case Studies from the Marine Phytoplankton

Medlin¹,

Töbe²

2011

Biodiversity

View full text Add to dashboard Cite

“…The AFLP technique developed by Vos et al (1995) was used as described in John et al (2004). Genomic DNA of 48 Phaeocystis strains previously extracted in earlier studies (Medlin et al 1994;Lange et al 2002) was digested over night at 37°C with two restriction enzymes (EcoRI and MseI, New England BioLabs, Frankfurt a.…”

Section: Ampliwed Fragment Length Polymorphismmentioning

confidence: 99%

Methods used to reveal genetic diversity in the colony-forming prymnesiophytes Phaeocystis antarctica, P. globosa and P. pouchetii—preliminary results

Gaebler

Hayes

Medlin

2007

Phaeocystis, Major Link in the Biogeochemical Cycling of Climate-Relevant Elements

View full text Add to dashboard Cite

Previous work on the genetic diversity of Phaeocystis used ribosomal DNA and internal transcribed spacer (ITS) sequence analyses to show that there is substantial inter-and intraspeciWc variation within the genus. First attempts to trace the biogeographical history of strains in Antarctic coastal waters were based on a comparison of ITS sequences. To gain deeper insights into the population structure and bloom dynamics of this microalga it is necessary to quantify the genetic diversity within populations of P. antarctica from diVerent locations (i.e., each of the three major gyres in the Antarctic continental waters) and to calculate the gene Xow between them. Here we describe methods to quantify genetic diversity and our preliminary results for P. antarctica in comparison to two other colonial species: P. globosa and P. pouchetii. For this study of genetic diversity, two Wngerprinting techniques were used. First, ampliWed fragment-length polymorphisms (AFLPs) were established as a pre-screening tool to assess clone diversity and to select divergent clones prior to physiological investigations. Second, the more-powerful microsatellite markers were established to assess population structure and biogeography more accurately. Results show diVerences in the AFLP patterns between isolates of P. antarctica from diVerent regions, and that a wide variety of microsatellite motifs could be obtained from the three Phaeocystis species.

show abstract

Utility of Amplified Fragment Length Polymorphisms (AFLP) to Analyse Genetic Structures within the Alexandrium tamarense Species Complex

Cited by 51 publications

References 32 publications

Characterization of Nontoxic and Toxin-Producing Strains ofAlexandrium minutum(Dinophyceae) in Irish Coastal Waters

Characterization of Nontoxic and Toxin-Producing Strains ofAlexandrium minutum(Dinophyceae) in Irish Coastal Waters

Molecular Techniques to Estimate Biodiversity with Case Studies from the Marine Phytoplankton

Methods used to reveal genetic diversity in the colony-forming prymnesiophytes Phaeocystis antarctica, P. globosa and P. pouchetii—preliminary results

Contact Info

Product

Resources

About