Molecular Techniques to Estimate Biodiversity with Case Studies from the Marine Phytoplankton

Medlin, Linda K.; Töbe, Kerstin

doi:10.5772/25265

Cited by 2 publications

(1 citation statement)

References 98 publications

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“…Metabarcoding based on high-throughput sequencing of the V9-18S rRNA gene allows for communities to be studied with high taxonomic resolution. Through this approach, uncultivated species (Johnson and Martiny, 2015;Medlin, et al, 2011), picoplankton species that lack distinct morphological structures (Caron et al, 2012;Massana, 2011;Santi et al, 2021), and spatiotemporal differences (Santoferrara et al, 2020) may be identified, resulting in a more accurate picture of microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Response of microbial eukaryote community to the oligotrophic waters of the Gulf of Mexico: a plausible scenario for warm and stratified oceans

Sidón-Ceseña,

Martínez-Mercado,

Chong-Robles

et al. 2023

Preprint

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In oligotrophic environments, interactions among eukaryotic microorganisms are highly complex. In the Gulf of Mexico (GoM), the Loop Current intensifies in summer and supplies the Gulf with warm and oligotrophic waters. However, mesoscale eddies within the GoM create favorable conditions for biological productivity by bringing nutrient-rich water to the subsurface layer. This study aimed to determine the structure, variability, and ecological roles of the protist in the mixed layer (ML) and deep chlorophyll maximum (DCM), representing the first V9-18S rRNA survey studying the protist community from the Southern GoM. Results revealed different assemblages between the ML and DCM. In the ML, species abundance was highly and positively correlated with temperature but negatively correlated with the nitrate concentration, whereas the opposite pattern was observed in the DCM. Alveolata represented ~60% in both the ML and DCM, while Haptophytes and MAST dominated the ML, and Pelagophytes and Radiolarians dominated the DCM. Interestingly, Ostreococcus abundance increased under upwelling conditions suggesting that it may act as an indicator of the vertical nitrate flux and that picoeukaryotes respond to this instead of diatoms. Finally, our analyses revealed high levels of competition, parasitism, and predation with a high proportion of self-exclusion relationship (30%) in both depths.

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Section: Introductionmentioning

confidence: 99%

Response of microbial eukaryote community to the oligotrophic waters of the Gulf of Mexico: a plausible scenario for warm and stratified oceans

Sidón-Ceseña,

Martínez-Mercado,

Chong-Robles

et al. 2023

Preprint

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Whole cell hybridisation for monitoring harmful marine microalgae

Toebe

2013

Environ Sci Pollut Res

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Fluorescence in situ hybridisation (FISH) is a powerful molecular biological tool to detect and enumerate harmful microorganism in the marine environment. Different FISH methods are available, and especially in combination with automated counting techniques, the potential for a routine monitoring of harmful marine microalgae is attainable. Various oligonucleotide probes are developed for detecting harmful microalgae. However, FISH-based methods are not yet regularly included in monitoring programmes tracking the presence of harmful marine microalgae. A limitation factor of the FISH technique is the currently available number of suited fluorochromes attached to the FISH probes to detect various harmful species in one environmental sample at a time. However, coupled automated techniques, like flow cytometry or solid-phase cytometry, can facilitate the analysis of numerous field samples and help to overcome this drawback. A great benefit of FISH in contrast to other molecular biological detection methods for harmful marine microalgae is the direct visualisation of the hybridised target cells, which are not permitted in cell free formats, like DNA depending analysis methods. Therefore, an additional validation of the FISH-generated results is simultaneously given.

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Molecular Techniques to Estimate Biodiversity with Case Studies from the Marine Phytoplankton

Cited by 2 publications

References 98 publications

Response of microbial eukaryote community to the oligotrophic waters of the Gulf of Mexico: a plausible scenario for warm and stratified oceans

Response of microbial eukaryote community to the oligotrophic waters of the Gulf of Mexico: a plausible scenario for warm and stratified oceans

Whole cell hybridisation for monitoring harmful marine microalgae

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