Utilising polyphenols for the clinical management of Candida albicans biofilms

Shahzad, Muhammad; Sherry, Leighann; Rajendran, Ranjith; Edwards, Christine; Combet, Emilie; Ramage, Gordon

doi:10.1016/j.ijantimicag.2014.05.017

Cited by 96 publications

(63 citation statements)

References 14 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the MIC 80 of QCT were generally > 200 µg/mL consistent with a previous report [19], and the antibiofilm effect of QCT alone was poor as the SMIC 50 were ≥ 1024 µg/mL (Table 2). However, QCT appeared to have strong synergism with FCZ in FCZ-resistant C. albicans isolates ( Table 2 and Fig.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Gao

Wang

Zhu

2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Vulvovaginal candidiasis (VVC) is a common gynecological disease. Candida albicans is believed to be mainly implicated in VVC occurrence, the biofilm of which is one of the virulence factors responsible for resistance to traditional antifungal agents especially to fluconazole (FCZ). Quercetin (QCT) is a dietary flavonoid and has been demonstrated to be antifungal against C. albicans biofilm. Methods: 17 C. albicans isolates including 15 clinical ones isolated from VVC patients were employed to investigate the effects of QCT and/or FCZ on the inhibition of C. albicans biofilm. Results: We observed that 64 µg/mL QCT and/or 128 µg/mL FCZ could (i) be synergistic against 10 FCZ-resistant planktonic and 17 biofilm cells of C. albicans, (ii) inhibit fungal adherence, cell surface hydrophobicity (CSH), flocculation, yeast-to-hypha transition, metabolism, thickness and dispersion of biofilms; (iii) down-regulate the expressions of ALS1, ALS3, HWP1, SUN41, UME6 and ECE1 and up-regulate the expressions of PDE2, NRG1 and HSP90, and we also found that (iv) the fungal burden was reduced in vaginal mucosa and the symptoms were alleviated in a murine VVC model after the treatments of 5 mg/kg QCT and/or 20 mg/kg FCZ. Conclusion: Together with these results, it could be demonstrated that QCT could be a favorable antifungal agent and a promising synergist with FCZ in the clinical management of VVC caused by C. albicans biofilm.

show abstract

Section: Discussionsupporting

confidence: 91%

“…QCT has been demonstrated to possess a series of functions as well as antifungal potentials [19,[32][33][34]. In this study, the MIC 80 of QCT were generally > 200 µg/mL consistent with a previous report [19], and the antibiofilm effect of QCT alone was poor as the SMIC 50 were ≥ 1024 µg/mL (Table 2).…”

Section: Discussionsupporting

confidence: 90%

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Gao

Wang

Zhu

2016

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Cinnamaldehyde was also known to inhibit growth of various microorganisms by affecting membrane integrity, energy generation and extended-spectrum beta-lactamase (ESBL) protein dependant drug resistance [17]- [19]. Curcumin is known to induce apoptosis in eukaryotic cells and also reported to inhibit planktonic and biofilm growth of C. albicans [20] [51]. Thus membrane integrity damage and apoptosis inducing activity of cinnamaldehyde and curcumin could be associated with anti-Candida activity in our study.…”

Section: Discussionmentioning

confidence: 52%

“…Synergistic activity was found to be dependent on accessibility of the cells i.e. very active against planktonic growth (accessible), followed by developing biofilm (less accessible) and mature biofilm (inaccessible) [3]- [5] [27] [51].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on <i>Candida albicans</i>

Thakre¹,

Mulange²,

Kodgire³

et al. 2016

AiM

View full text Add to dashboard Cite

Efficacy of five plant molecules against thirty three clinical isolates and two standard strains of C. albicans, differentially susceptible to fluconazole (FLC) is tested in this study. Effect on biofilm (adhesion, development and maturation) formation, morphogenesis and synergy with fluconazole (FLC) against a FLC resistant strain of Candida albicans ATCC 10231 is also evaluated. All the plant molecules tested were equally effective against isolates and strains of C. albicans (N = 35) tested in this study. Cinnamaldehyde was found most effective against planktonic growth followed by ocimene. Both the molecules exhibited fungicidal activity and killed 99.9% of inoculum within 80 and 20 min of exposure respectively at 0.62 mM and 176.8 mM concentrations. Curcumin (5 -20 mM), camphene (8 -32 mM) and farnesene (25 -100 mM), although inhibited planktonic growth, were fungistatic. All the five plant molecules tested in this study inhibited morphogenesis significantly and exhibited considerable activity against biofilm formation. Inhibition of biofilm was found to be stage specific i.e. efficacy was more against adhesion followed by developing and mature biofilm. Plant molecules tested exhibited excellent synergy with fluconazole. However FIC index values 0.155, 0.062 and 0.046 indicate that ocimene was the most effective synergistic molecule inhibited planktonic growth, developing biofilm and mature biofilm growth respectively at very low concentrations. This is the first report of anti-Candida activity of three terpenoids viz. ocimene, farnesene and camphene against planktonic & biofilm growth, morphogenesis as well as synergy with FLC. Plant molecules tested in this study may find use in antifungal chemotherapy individually and or in a combination with FLC.

show abstract

“…The current treatment options for fungal biofilm-related infections are very scarce due to the intrinsic increased tolerance of biofilms to antimycotics (Bink et al, 2011); hence there is a novel trend towards the identification of natural products, plants and their extracts with anti-biofilm activity. In this respect several natural compounds and/or plant extracts were reported in literature to prevent the C. albicans biofilm formation, most of them are polyphenols (Shahzada et al, 2014). Recently, De Vita et al (2014) investigated caffeic acid and its ester derivatives for their capacity to inhibit the biofilm formation.…”

Section: Antibiofilm Activitymentioning

confidence: 99%

Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves

Scognamiglio

Buommino

Coretti

et al. 2016

Phytochemistry Letters

View full text Add to dashboard Cite

Utilising polyphenols for the clinical management of Candida albicans biofilms

Cited by 96 publications

References 14 publications

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on <i>Candida albicans</i>

Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves

Contact Info

Product

Resources

About

Utilising polyphenols for the clinical management of Candida albicans biofilms

Cited by 96 publications

References 14 publications

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on &lt;i&gt;Candida albicans&lt;/i&gt;

Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves

Contact Info

Product

Resources

About

Effects of Cinnamaldehyde, Ocimene, Camphene, Curcumin and Farnesene on <i>Candida albicans</i>