Uterine Environment in Early Pregnancy

Fischer, Bernd; Beier, Henning M.

doi:10.1007/978-94-009-5038-2_6

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…It is known from various species that ULF contains blood proteins, as well as proteins secreted by the uterine epithelium (Fischer & Beier 1986, Kayser et al 2006, Lee et al 1998, Roberts & Parker 1974. Therefore, a quantitative proteome study was performed in order to detect proteins differing in abundance between both biological fluids (Faulkner et al 2012).…”

Section: Modulation Of the Ulf Proteome During Oestrous Cyclementioning

confidence: 99%

Proteomics of bovine endometrium, oocytes and early embryos

Deutsch¹,

Fröhlich²,

Arnold³

2019

Proc

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Embryo-maternal communication, as well as recognition and establishment of pregnancy, is predominantly controlled by secretion of hormones and proteins. Furthermore, uterine fluid serves as a histotroph and contains factors essential for embryo development and elongation, making it a meaningful source for proteomic approaches of early embryonic development. The studies included in this review address the analysis of uterine fluid and endometrium during the oestrous cycle, early pregnancy, maternal effects on embryo development and embryo-induced alterations to the maternal environment. Clear differences between the proteome of uterine fluid and blood plasma unravelled the active contribution of the endometrium to the composition of uterine luminal fluid. Proteins were identified which may contribute either to growth enhancement of blastocysts or influence secretion of "Interferon tau". Several studies suggested important roles of intrauterine factors including "Tissue inhibitor of metalloproteinases 2" for pregnancy establishment and "Acyl-CoA-binding protein" for the embryo prior to implantation. A meta-analysis of quantitative proteome studies revealed functional clusters including the terms "degradation of extracellular matrix", "platelet degranulation" and "MHC class II antigen presentation". Proteomic studies targeting oocytes and early embryos provided evidence for the function of "Cyclin B" in mitotic resumption, for "14-3-3 epsilon" to activate "maturation promotion factor" for promotion of mitosis during in vitro maturation, or for the inhibition of proteasomes during the late phase of maturation. Analysis of blastocyst derived trophectoderm cell lines suggested effects of annexin down-regulation on the failure rate of nuclear transfer pregnancies. Finally, absolute quantification of "Y-box-binding protein 2" and "Insulin-like growth factor 2 mRNA-binding protein 3" during early embryogenesis by "selected reaction monitoring" demonstrated the dynamism of proteome alterations during the first embryonic cell divisions.

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Section: Modulation Of the Ulf Proteome During Oestrous Cyclementioning

confidence: 99%

Proteomics of bovine endometrium, oocytes and early embryos

Deutsch¹,

Fröhlich²,

Arnold³

2019

Proc

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“…Early embryo losses, defined from fertilisation to implantation, have been estimated at around 15% [6] and they may result of an inherited abnormal development, an asynchronism between the stage of embryo development and the uterine environment, or to an inadequate steroidogenic pattern [14,37].…”

Section: Embryo Developmentmentioning

confidence: 99%

Embryo Manipulation Techniques in the Rabbit

García¹

2018

New Insights Into Theriogenology

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Rabbits are both productive and classic laboratory animals. Some particularities of female reproductive physiology make the rabbit an extraordinary model for the study of embryology and assisted reproductive techniques. For instance, as the ovulation is induced, the embryo development can be known with accuracy. Embryos are surrounded by a mucin coat which is crucial to prevent embryo mortality. Besides, the anatomy of the uterus does not allow embryo transmigration between both uterine horns, and so it is possible to test different reproductive techniques. Knowledge on early embryo development, and on influencing factors, has allowed to develop new insights into embryo manipulation, such as recovery, transfer, cryopreservation, in vitro fertilisation, cloning, or transgenesis. Also the rabbit may be used as a model for human reproductive health, because rabbit embryo and feto-placental development are similar to the human. This chapter reviews the aspects of the reproductive physiology in the female rabbit and discusses some embryo manipulation techniques available in the species.

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“…The changes in the uterine luminal fluid proteins (ULFP) appear to be necessary act as protease inhibitors (Fazleabas et al, 1982) [10] enzymes (Hansen et al, 1985) [21] carrier molecules for hormone, vitamin, and mineral (Zavy et al, 1979: Buchi et al, 1982: Pentacost and Tang, 1987 [43,7,34] pregnancy recognition signalling molecules (Godkin et al, 1984;Bartol et al, 1985;Godkin et al, 1988) [17,34,18] immunoregulatory molecules (Murray et al, 1978) [31] as well as several more likely unidentified roles. Some ULFPs are synthesized from the uterine endometrium, while the majority are sequestered from the blood into the uterine lumen (Fischer and Beier, 1986) [11] . The significant variations in the ULFPs have been seen in mares during the estrous cycle (Zavy et al, 1978;Zavy et al, 1979) [43,44] , pigs (Kayser et al, 2006) [25] , buffaloes (Kumar and Purohit, 2018) [26] , cows (Alavi-Shoushtari et al, 2006; Alavi-Shoushtari et al, 2014) [3,2] .…”

Section: Introductionmentioning

confidence: 99%

Uterine luminal fluid proteins in goats during various reproductive stages (Follicular stage, luteal stage, and early pregnancy)

Pannu,

Purohit,

Pateer

2024

Int. J. Adv. Biochem. Res.

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The purpose of this study was to assess the changes in uterine luminal fluid proteins during various reproductive stages using goat genitalia obtained from a slaughterhouse. Three groups of genital tracts were classified: early pregnancy (n = 13), luteal phase (n = 21), and follicular phase (n = 26). After the genitalia were dissected, Eppendorf tubes were used to gather samples of uterine fluid. The uterine fluid samples were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis examination. The molecular weights of the protein bands were determined by comparing their migration rates on the gel with those of protein markers that had known molecular weights. Molecular

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Uterine Environment in Early Pregnancy

Cited by 11 publications

References 30 publications

Proteomics of bovine endometrium, oocytes and early embryos

Proteomics of bovine endometrium, oocytes and early embryos

Embryo Manipulation Techniques in the Rabbit

Uterine luminal fluid proteins in goats during various reproductive stages (Follicular stage, luteal stage, and early pregnancy)

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