Uterine luminal fluid proteins in goats during various reproductive stages (Follicular stage, luteal stage, and early pregnancy)

Abstract

The purpose of this study was to assess the changes in uterine luminal fluid proteins during various reproductive stages using goat genitalia obtained from a slaughterhouse. Three groups of genital tracts were classified: early pregnancy (n = 13), luteal phase (n = 21), and follicular phase (n = 26). After the genitalia were dissected, Eppendorf tubes were used to gather samples of uterine fluid. The uterine fluid samples were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis examination. The molecular weights of the protein bands were determined by comparing their migration rates on the gel with those of protein markers that had known molecular weights. Molecular