Using the radioligand-receptor binding assay for paralytic shellfish toxins: A case study on shellfish from Morocco

Naouli, J.; Abouabdellah, Rachid; Bennouna, Asmae; Laissaoui, A.; Swarzenski, Peter W.; Bouh, H. Ait; Mesfioui, Abdelhakim; Benbrahim, M.-S.; Bottein, Marie‐Yasmine Dechraoui

doi:10.1016/j.jenvrad.2018.07.020

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…Besides, MCs, NODs and CYNs were also found in several riverside countries along the Limpopo River [173]. STXs were reported in Morocco [174] and Nigeria [171]. ATXs were distributed in Kenya [175] and Nigeria [171].…”

Section: Distribution Of Cyanotoxinsmentioning

confidence: 99%

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Liu

Yuan

et al. 2019

Toxins

126

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The widespread distribution of cyanobacteria in the aquatic environment is increasing the risk of water pollution caused by cyanotoxins, which poses a serious threat to human health. However, the structural characterization, distribution and identification techniques of cyanotoxins have not been comprehensively reviewed in previous studies. This paper aims to elaborate the existing information systematically on the diversity of cyanotoxins to identify valuable research avenues. According to the chemical structure, cyanotoxins are mainly classified into cyclic peptides, alkaloids, lipopeptides, nonprotein amino acids and lipoglycans. In terms of global distribution, the amount of cyanotoxins are unbalanced in different areas. The diversity of cyanotoxins is more obviously found in many developed countries than that in undeveloped countries. Moreover, the threat of cyanotoxins has promoted the development of identification and detection technology. Many emerging methods have been developed to detect cyanotoxins in the environment. This communication provides a comprehensive review of the diversity of cyanotoxins, and the detection and identification technology was discussed. This detailed information will be a valuable resource for identifying the various types of cyanotoxins which threaten the environment of different areas. The ability to accurately identify specific cyanotoxins is an obvious and essential aspect of cyanobacterial research.

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Section: Distribution Of Cyanotoxinsmentioning

confidence: 99%

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Liu

Yuan

et al. 2019

Toxins

126

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“…To our knowledge, this is the first report of occurrence of GTX-2 and C1 toxin in Boughrara lagoon. These findings serve to draw attention to this potential threat and to provide the information necessary to ensure the biotoxin-monitoring program continue in order to produce a complete risk assessment especially if it is known that, recently, several severe cases of human intoxication have been recorded in Morocco following consumption of shellfish contaminated by paralytic shellfish toxins [29].…”

Section: Hydrophilic and Lipophilic Biotoxins Detected In Clam Samplesmentioning

confidence: 99%

Marine Biotoxin Profile and Karenia selliformis and Alexandrium minitum Occurrence in Boughrara Lagoon During the Last Decade

Marrouchi

Abdennadher

Feki-Sahnoun

et al. 2022

Ecological Chemistry and Engineering S

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As part of the routine control of the Tunisian network for the monitoring of the most problematic microalgae species Karenia selliformis and Alexandrium minutum and for the study of hydrophilic and lipophilic marine biotoxin profiles, clams and seawater samples from Boughrara lagoon were analysed during the past decade. The results showed that the abundance of Karenia selliformis and Alexandrium minutum in the Boughrara lagoon exhibited significant variability at annual scales. K. selliformis was recorded in all sampling years with interannual variability. Among the nine sampled years, 2010, 2012, 2016 and 2017 were, respectively, the most significant in terms of mean abundance (2.85, 2.42, 1.38, 1.69) ∙ 105 cells L–1. A. minutum species were observed in the water column during all investigated years except in 2013, 2016 and 2017, but in low concentrations compared to K. selliformis (≤ 7.4 ∙ 102 cells L–1). Using LC-FLD, traces of paralytic toxins (C1 and GTX-2) were detected in some clam samples. Their concentrations were largely below the regulatory limit. For the first time, N-sulfocarbamoyl gonyautoxin-2 (C-1) and gonyautoxin 2 (GTX-2) were detected in some clam samples from this region. LC-MS/MS analysis confirmed the presence of gymnodimines in some samples. The main peak corresponds to the GYM-A with high concentrations generally above 1 mg kg–1 of clams meat, which confirms the persistence of this neurotoxin in Boughrara lagoon.

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“…Several new methods for MBs analysis have been published. − Notwithstanding, most of these methods present high complexity for sample preparation and/or high costs. Moreover, sometimes more than one technique or analytical approach is necessary to analyze a comprehensive scope of biotoxins.…”

Section: Introductionmentioning

confidence: 99%

Cost-Effective and High-Reliability Analytical Approach for Multitoxin Screening in Bivalve Mollusks by Liquid Chromatography Coupled to Tandem Mass Spectrometry

Molognoni

Santos

Kleemann

et al. 2019

J. Agric. Food Chem.

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A fast, less expensive, analytical approach with high metrologic reliability was developed to assist an official program for 21 marine biotoxins, monitoring in bivalve mollusks. The simultaneous analysis of lipophilic and hydrophilic marine biotoxins was achieved using a sample preparation protocol based on solid–liquid extraction and low-temperature cleanup, followed by liquid chromatography coupled to tandem mass spectrometry. Samples were extracted with acidified methanol/water (90:10), followed by low-temperature cleanup. Chromatographic separation was obtained using a cyano-bonded silica phase. The mobile phase was composed of water and acetonitrile, with both 0.1% formic acid and 2.5 mmol L–1 ammonium formate. Electrospray ionization was used in both negative and positive modes. The single-laboratory validation approach enabled method performance assessment, and the necessary data to design a model for result expression were yielded. With this purpose, a systematic study of errors and uncertainties was performed. This new analytical approach aimed to minimize the use of highly expensive analytical standards, promoting economic viability to be applied by high-throughput routine laboratories. After its implementation on the Brazilian official monitoring program, positive results near the regulatory limits were obtained, demonstrating the fit for purpose of the method as a surveillance tool.

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Using the radioligand-receptor binding assay for paralytic shellfish toxins: A case study on shellfish from Morocco

Cited by 7 publications

References 12 publications

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Marine Biotoxin Profile and Karenia selliformis and Alexandrium minitum Occurrence in Boughrara Lagoon During the Last Decade

Cost-Effective and High-Reliability Analytical Approach for Multitoxin Screening in Bivalve Mollusks by Liquid Chromatography Coupled to Tandem Mass Spectrometry

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