Marine Biotoxin Profile and Karenia selliformis and Alexandrium minitum Occurrence in Boughrara Lagoon During the Last Decade

Abstract

As part of the routine control of the Tunisian network for the monitoring of the most problematic microalgae species Karenia selliformis and Alexandrium minutum and for the study of hydrophilic and lipophilic marine biotoxin profiles, clams and seawater samples from Boughrara lagoon were analysed during the past decade. The results showed that the abundance of Karenia selliformis and Alexandrium minutum in the Boughrara lagoon exhibited significant variability at annual scales. K. selliformis was recorded in all sampling years with interannual variability. Among the nine sampled years, 2010, 2012, 2016 and 2017 were, respectively, the most significant in terms of mean abundance (2.85, 2.42, 1.38, 1.69) ∙ 105 cells L–1. A. minutum species were observed in the water column during all investigated years except in 2013, 2016 and 2017, but in low concentrations compared to K. selliformis (≤ 7.4 ∙ 102 cells L–1). Using LC-FLD, traces of paralytic toxins (C1 and GTX-2) were detected in some clam samples. Their concentrations were largely below the regulatory limit. For the first time, N-sulfocarbamoyl gonyautoxin-2 (C-1) and gonyautoxin 2 (GTX-2) were detected in some clam samples from this region. LC-MS/MS analysis confirmed the presence of gymnodimines in some samples. The main peak corresponds to the GYM-A with high concentrations generally above 1 mg kg–1 of clams meat, which confirms the persistence of this neurotoxin in Boughrara lagoon.

Measuring Marine Biotoxins in a Hypersaline Coastal Lagoon

“…The objective of this study is to measure these toxins in a hypersaline coastal lagoon that is experiencing severe eutrophication to better understand the processes keeping them in the water. There are a few records of in-water marine biotoxins in Mediterranean coastal lagoons [ 27 , 28 , 29 , 30 , 31 ]. The Mar Menor is the largest hypersaline coastal lagoon in Europe, and is a popular tourist area, with large fisheries, and although it is not a shellfish cultivation area, cockles and sea snails are frequently consumed by local residents.…”