Using spectroscopic and microscopic methods to probe the structural stability of human adenovirus type 4

Characterization of an Oncolytic Herpes Simplex Virus Drug Candidate

“…The decrease in light scattering intensity may also reflect a change in the differential refractive index (d n /d c ). This decrease in light scattering intensity has been previously observed in human adenovirus type 4, measles virus, and filovirus virus‐like particles (VLPs) . In these studies, as well as the current study, there is no direct evidence to support or disprove the precise mechanism.…”

“…The accessibly of viral DNA to solution was measured as a function of temperature by using the nucleic acid binding dye, PI . PI cannot penetrate the intact viral membrane and is essentially non‐fluorescent when not bound to DNA.…”

“…The accessibility of viral DNA was measured using propidium iodide (PI) (Life Technologies), which is a membrane impermeable dye that binds the major groove of double stranded DNA (dsDNA) . PI (suspended in ultrapure water) was mixed with o‐HSV at a final concentration of 20 μM.…”

“…The accessibility of viral DNA was measured using propidium iodide (PI) (Life Technologies), which is a membrane impermeable dye that binds the major groove of double stranded DNA (dsDNA). 30,31 PI (suspended in ultrapure water) was mixed with o-HSV at a final concentration of 20 :M. Duplicate samples were measured using an excitation wavelength of 535 nm with the emission spectra monitored from 550 to 650 nm as a function of temperature (10 • C-87.5 • C) and pH (5.5-8). The excitation and emission slits were set at 4 nm.…”

Subunit Vaccine Delivery

“…Similar physical characterization has been performed with different rotavirus serotypes ) and multiple types of adenoviruses (Rexroad et al 2006a ;Rexroad et al 2006b ;Rexroad et al 2003 ;He et al 2010 ). …”