Using source-associated mobile genetic elements to identify zoonotic extraintestinal E. coli infections

Liu, Cindy M.; Aziz, Maliha; Park, Daniel E.; Wu, Zhenke; Stegger, Marc; Li, Mengbing; Wang, Yashan; Schmidlin, Kara; Johnson, Timothy J.; Koch, Benjamin J.; Hungate, Bruce A.; Nordstrom, Lora; Gauld, Lori; Weaver, Brett; Rolland, Diana; Statham, Sally; Hall, Brantley; Sariya, Sanjeev; Davis, Gregg S.; Keim, Paul; Johnson, James R.; Price, Lance B.

doi:10.1016/j.onehlt.2023.100518

Cited by 18 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ST131-B has been previously widely indicated as a foodborne uropathogen, in particular related to consumption of poultry meat (39,40). Combined, this evidence indicates that both ST95 and ST131-B lineages circulating in human hosts are stably split into human vs. avian adapted subclades assisted by their plasmid-associated traits, and that the latter likely reflects a constant spillover from the avian niche via foodborne transmission.…”

Section: Discussionmentioning

confidence: 75%

“…In both evolutionary backgrounds, these clades were non-overlapping with the clades hosting pColV-like plasmid, and have been found to be strongly associated with avian pathogenic E. coli 25,38,52 . ST131-B has been previously widely indicated as a foodborne uropathogen, in particular related to consumption of poultry meat 53,54 . Combined, this evidence indicates that ST95 and ST131-B lineages circulating in human hosts are stably divided into human vs. avian adapted subclades assisted by their plasmid-associated traits, and that the latter likely reflects a constant spillover from the avian niche via foodborne transmission.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Plasmid-driven strategies for clone success inEscherichia coli

Arredondo-Alonso,

Pöntinen,

Gama

et al. 2023

Preprint

View full text Add to dashboard Cite

Escherichia coli is a major human pathogen and the most widely studied microbe in history, but its extrachromosomal elements known as plasmids remain poorly delineated. Here we used long-read technology to high-resolution sequence the entire plasmidome and the corresponding host chromosomes from a longitudinal survey covering two decades and over 2,000 E. coli isolates. Separation of chromosomal and extrachromosomal DNA enabled us to reconstruct co-evolutionary trajectories of host lineages and their plasmids on a population-wide scale, demonstrating that plasmid evolution is markedly constrained contrary to the established dogma. We find that some plasmids have persisted in lineages for centuries, and provide a high-resolution map of recent vertical and horizontal evolutionary events in plasmids with key antibiotic resistance, competition and virulence determinants. We present genomic evidence of both chromosomal and plasmid-driven success strategies that represent convergent phenotypic evolution in distant lineages, and use in vitro experiments to verify the importance of bacteriocin-producing plasmids for clone success. Our study has general implications for understanding plasmid biology and bacterial evolutionary strategies, and informing development of future interventions against pathogens.

show abstract

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Plasmid-driven strategies for clone success inEscherichia coli

Arredondo-Alonso,

Pöntinen,

Gama

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This horizontal gene transfer allows bacteria to acquire new resistance mechanisms, leading to the emergence of multidrug-resistant strains that pose a serious threat to public health. The ability of mobile genetic elements to transfer resistance genes between bacteria highlights the importance of understanding and monitoring the spread of these elements in combating antibiotic resistance in E. coli and other pathogenic bacteria ( Liu et al., 2023 and Jeon et al., 2023 ).…”

Section: E Coli Biofilms and Antibiotic Resistancementioning

confidence: 99%

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli

Nasrollahian,

Graham,

Halaji

2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The dissemination of antibiotic resistance in Escherichia coli poses a significant threat to public health worldwide. This review provides a comprehensive update on the diverse mechanisms employed by E. coli in developing resistance to antibiotics. We primarily focus on pathotypes of E. coli (e.g., uropathogenic E. coli) and investigate the genetic determinants and molecular pathways that confer resistance, shedding light on both well-characterized and recently discovered mechanisms. The most prevalent mechanism continues to be the acquisition of resistance genes through horizontal gene transfer, facilitated by mobile genetic elements such as plasmids and transposons. We discuss the role of extended-spectrum β-lactamases (ESBLs) and carbapenemases in conferring resistance to β-lactam antibiotics, which remain vital in clinical practice. The review covers the key resistant mechanisms, including: 1) Efflux pumps and porin mutations that mediate resistance to a broad spectrum of antibiotics, including fluoroquinolones and aminoglycosides; 2) adaptive strategies employed by E. coli, including biofilm formation, persister cell formation, and the activation of stress response systems, to withstand antibiotic pressure; and 3) the role of regulatory systems in coordinating resistance mechanisms, providing insights into potential targets for therapeutic interventions. Understanding the intricate network of antibiotic resistance mechanisms in E. coli is crucial for the development of effective strategies to combat this growing public health crisis. By clarifying these mechanisms, we aim to pave the way for the design of innovative therapeutic approaches and the implementation of prudent antibiotic stewardship practices to preserve the efficacy of current antibiotics and ensure a sustainable future for healthcare.

show abstract

“…The characterisation of large numbers of isolates from diverse sources and locations, by multilocus sequence typing (MLST) and other methods [7], has revealed considerable genetic differentiation among bacteria from different hosts. Where there is clear association with a particular host or source reservoir it may be possible to identify the origin of human infections, for example, with Escherichia coli [8, 9], Salmonella [10], Streptococcus suis [11], Staphylococcus [12] and Campylobacter [13, 14]. In Campylobacter species and strains vary in the range of hosts from which they have been isolated.…”

Section: Introductionmentioning

confidence: 99%

“…example, with Escherichia coli [8,9], Salmonella [10], Streptococcus suis [11], Staphylococcus [12] and Campylobacter [13,14]. In Campylobacter species and strains vary in the range of hosts from which they have been isolated.…”

mentioning

confidence: 99%

Evidence of potential Campylobacter jejuni zooanthroponosis in captive macaque populations

Zang,

Pascoe,

Mourkas

et al. 2023

Microbial Genomics

View full text Add to dashboard Cite

Non-human primates share recent common ancestry with humans and exhibit comparable disease symptoms. Here, we explored the transmission potential of enteric bacterial pathogens in monkeys exhibiting symptoms of recurrent diarrhoea in a biomedical research facility in China. The common zoonotic bacterium Campylobacter jejuni was isolated from macaques (Macaca mulatta and Macaca fascicularis) and compared to isolates from humans and agricultural animals in Asia. Among the monkeys sampled, 5 % (44/973) tested positive for C. jejuni , 11 % (5/44) of which displayed diarrhoeal symptoms. Genomic analysis of monkey isolates, and 1254 genomes from various sources in Asia, were used to identify the most likely source of human infection. Monkey and human isolates shared high average nucleotide identity, common MLST clonal complexes and clustered together on a phylogeny. Furthermore, the profiles of putative antimicrobial resistance genes were similar between monkeys and humans. Taken together these findings suggest that housed macaques became infected with C. jejuni either directly from humans or via a common contamination source.

show abstract

Using source-associated mobile genetic elements to identify zoonotic extraintestinal E. coli infections

Cited by 18 publications

References 27 publications

Plasmid-driven strategies for clone success inEscherichia coli

Plasmid-driven strategies for clone success inEscherichia coli

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli

Evidence of potential Campylobacter jejuni zooanthroponosis in captive macaque populations

Contact Info

Product

Resources

About