Using
            <scp>CRISPR</scp>
            ‐based gene drive for agriculture pest control

Courtier‐Orgogozo, Virginie; Morizot, Baptiste; Boëte, Christophe

doi:10.15252/embr.201744822

Cited by 9 publications

(11 citation statements)

References 6 publications

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“…2A). For any induction times less than 10 h, the gene drive activity (18,19) remained below 50% (Fig. 3D).…”

Section: Percent Gene Drive Activitymentioning

confidence: 95%

“…However, for isolates subjected to galactose induction (5 h), only the two PCRs corresponding to the gene drive allele (A, B) were present; reactions for the target allele were unable to amplify the expected fragment (C, D). A similar analysis was performed for eight clonal isolates from the NES strains (18,19). However, we sampled two isolates that were sensitive and two samples resistant to the SD-HIS condition; diagnostic PCRs illustrated that the target allele was still present for surviving colonies (Fig.…”

Section: Percent Gene Drive Activitymentioning

confidence: 99%

“…There are numerous applications of gene drive biotechnology to control and alter biological populations including global challenges such as eliminating insect-borne diseases [16][17][18] . Recent experimental [19][20][21][22][23][24] and computational studies [25][26][27] highlight the potential of GD systems.…”

Section: Introductionmentioning

confidence: 99%

“…Below, A similar analysis of clonal isolates from the NES-containing strains was performed following drive activation (24 h). Two isolates each from(18) and(19) were chosen that were resistant or sensitive to the SD-HIS condition. (F) The gene drive activities from all measured conditions in (C, D) were plotted.…”

mentioning

confidence: 99%

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Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae

Goeckel

Basgall

Lewis

et al. 2018

Preprint

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The bacterial CRISPR/Cas genome editing system has provided a major breakthrough in molecular biology. One use of this technology is within a nuclease-based gene drive. This type of system can install a genetic element within a population at unnatural rates. Combatting of vectorborne diseases carried by metazoans could benefit from a delivery system that bypasses traditional Mendelian laws of segregation. Recently, laboratory studies in fungi, insects, and even mice, have demonstrated successful propagation of CRISPR gene drives and the potential utility of this type of mechanism. However, current gene drives still face challenges including evolved resistance, containment, and the consequences of application in wild populations. In this study, we use an artificial gene drive system in budding yeast to explore mechanisms to modulate nuclease activity of Cas9 through its nucleocytoplasmic localization. We examine non-native nuclear localization sequences on Cas9 fusion proteins in vivo and demonstrate that appended signals can titrate gene drive activity and serve as a potential molecular safeguard.

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“…2A). For any induction times less than 10 h, the gene drive activity (18,19) remained below 50% (Fig. 3D).…”

Section: Percent Gene Drive Activitymentioning

confidence: 95%

Section: Percent Gene Drive Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae

Goeckel

Basgall

Lewis

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…This super-Mendelian genetic arrangement allows for the forced propagation of a genetic element within a population and has the potential to modify entire species on a global scale (BULL AND BARRICK 2017;GODFRAY et al 2017). Some of the possible benefits of this technology include eradication of invasive species (ESVELT AND GEMMELL 2017;PROWSE et al 2017), agricultural pest management (COURTIER-ORGOGOZO et al 2017), and elimination of insect-borne diseases such as malaria (GODFRAY et al 2017;LAMBERT et al 2018). A number of recent studies have demonstrated the potency and success of CRISPR-based gene drives in fungi (DICARLO et al 2015;ROGGENKAMP et al 2017;ROGGENKAMP et al 2018;SHAPIRO et al 2018), and metazoans (GANTZ et al 2015;HAMMOND et al 2016;CHAMPER et al 2017;GRUNWALD et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Development of a multi-locus CRISPR gene drive system in budding yeast

Yan

Finnigan

2018

Preprint

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The discovery of CRISPR/Cas gene editing has allowed for major advances in many biomedical disciplines and basic research. One arrangement of this biotechnology, a nuclease-based gene drive, can rapidly deliver a genetic element through a given population and studies in fungi and metazoans have demonstrated the success of such a system. This methodology has the potential to control biological populations and contribute to eradication of insect-borne diseases, agricultural pests, and invasive species. However, there remain challenges in the design, optimization, and implementation of gene drives including concerns regarding biosafety, containment, and control/inhibition. Given the numerous gene drive arrangements possible, there is a growing need for more advanced designs. In this study, we use budding yeast to develop an artificial multi-locus gene drive system. Our minimal setup requires only a single copy of S. pyogenes Cas9 and three guide RNAs to propagate three separate gene drives. We demonstrate how this system could be used for targeted allele replacement of native genes and to suppress NHEJ repair systems by modifying DNA Ligase IV. A multi-locus gene drive configuration provides an expanded suite of options for complex attributes including pathway redundancy, combatting evolved resistance, and safeguards for control, inhibition, or reversal of drive action.

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Knowledge Training System of Urban Pest Control Based on Big Data Analysis

Zhang

2020

Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering

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Urban insects are a large group of arthropods closely related to urban economic construction and people’s life. If they are not effectively controlled, they will bring harm and loss to urban economic construction and people’s life. In this context, in order to help the relevant urban management departments to better complete the work of urban pest control, the design of urban pest control knowledge training system based on big data analysis is studied. The research includes the overall design and detailed design of the system. The overall system discusses the overall system architecture, system function architecture, system architecture, system topology and other macro or surface theories. The technical points of the whole training information management system are in the detailed design stage, which is divided into system login configuration module, training demand management module, resource management module, teaching management module, performance management module, system management module and other modules. Finally, the system test and performance analysis are carried out for the system. The results show that the training system can support 500 users to use simultaneously and meet the needs of users’ learning and training.

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Using CRISPR ‐based gene drive for agriculture pest control

Abstract: Response by the authors.

Cited by 9 publications

References 6 publications

Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae

Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae

Development of a multi-locus CRISPR gene drive system in budding yeast

Knowledge Training System of Urban Pest Control Based on Big Data Analysis

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