Using Reverse Genetics to Improve Influenza Vaccines

Elderfield, Ruth A.; Hartgroves, Lorian C.; Barclay, Wendy S.

doi:10.1002/9781118405338.ch8

Cited by 2 publications

(2 citation statements)

References 98 publications

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“…It might also help to manipulate the genetic reassortment process in order to improve the generation, selection, and production of high yielding vaccinal seeds [62]. …”

Section: Discussionmentioning

confidence: 99%

“…Understanding the fundamental molecular mechanisms that underlie packaging of the IAV genome is of the utmost importance since it should lead, in the long term, to a better understanding of the mechanisms sustaining genetic reassortment of IAVs [ 9 , 38 ] and help to assess the likelihood of the appearance of reassortant viruses, including those with pandemic potential. It might also help to manipulate the genetic reassortment process in order to improve the generation, selection, and production of high yielding vaccinal seeds [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

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Experimental Approaches to Study Genome Packaging of Influenza A Viruses

Isel

Munier

Naffakh

2016

Viruses

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The genome of influenza A viruses (IAV) consists of eight single-stranded negative sense viral RNAs (vRNAs) encapsidated into viral ribonucleoproteins (vRNPs). It is now well established that genome packaging (i.e., the incorporation of a set of eight distinct vRNPs into budding viral particles), follows a specific pathway guided by segment-specific cis-acting packaging signals on each vRNA. However, the precise nature and function of the packaging signals, and the mechanisms underlying the assembly of vRNPs into sub-bundles in the cytoplasm and their selective packaging at the viral budding site, remain largely unknown. Here, we review the diverse and complementary methods currently being used to elucidate these aspects of the viral cycle. They range from conventional and competitive reverse genetics, single molecule imaging of vRNPs by fluorescence in situ hybridization (FISH) and high-resolution electron microscopy and tomography of budding viral particles, to solely in vitro approaches to investigate vRNA-vRNA interactions at the molecular level.

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“…It might also help to manipulate the genetic reassortment process in order to improve the generation, selection, and production of high yielding vaccinal seeds [62]. …”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Experimental Approaches to Study Genome Packaging of Influenza A Viruses

Isel

Munier

Naffakh

2016

Viruses

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Developments in Plant Negative-Strand RNA Virus Reverse Genetics

Jackson

2016

Annu. Rev. Phytopathol.

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Twenty years ago, breakthroughs for reverse genetics analyses of negative-strand RNA (NSR) viruses were achieved by devising conditions for generation of infectious viruses in susceptible cells. Recombinant strategies have subsequently been engineered for members of all vertebrate NSR virus families, and research arising from these advances has profoundly increased understanding of infection cycles, pathogenesis, and complexities of host interactions of animal NSR viruses. These strategies also permitted development of many applications, including attenuated vaccines and delivery vehicles for therapeutic and biotechnology proteins. However, for a variety of reasons, it was difficult to devise procedures for reverse genetics analyses of plant NSR viruses. In this review, we discuss advances that have circumvented these problems and resulted in construction of a recombinant system for Sonchus yellow net nucleorhabdovirus. We also discuss possible extensions to other plant NSR viruses as well as the applications that may emanate from recombinant analyses of these pathogens.

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Using Reverse Genetics to Improve Influenza Vaccines

Cited by 2 publications

References 98 publications

Experimental Approaches to Study Genome Packaging of Influenza A Viruses

Experimental Approaches to Study Genome Packaging of Influenza A Viruses

Developments in Plant Negative-Strand RNA Virus Reverse Genetics

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