Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars

Stiles, John I.; Lemme, C.; Sondur, Suresh N.; Morshidi, M. B.; Manshardt, Richard M.

doi:10.1007/bf00225007

Cited by 141 publications

(68 citation statements)

References 8 publications

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“…Especially within the C. papaya accessions, limited variation was detected due to high level of similarity among these genotypes. This result is in agreement with other studies (STILES et al, 1993;MORSHIDI, 1998).…”

Section: Gs Xy = 2n Xy N X + N Ysupporting

confidence: 94%

“…The AFLP technique is more efficient, has better resolution for assessing genetic diversity, is inexpensive and requires less labor than the PCR-RFLP technique ( VAN DROOGENBROECK et al, 2004), In addition, the number of AFLP polymorphic bands obtained per primer combination is higher than those reported for RAPD markers (JÓBIN-DÉCOR et al, 1997;STILES et al, 1993;SONDUR et al, 1996). In the present study, a rate of 34.8 AFLP markers/primer enzyme combinations was obtained.…”

Section: Cluster Analysismentioning

confidence: 40%

“…In the case of papaya, some studies were conducted to characterize genetic diversity using isozymes (MORSHIDI, 1998), Random amplification of polymorphic DNA -RAPD (STILES et al, 1993;SONDUR et al, 1996;JOBIN-DÉCOR et al, 1997), Restriction Fragment Length Polymorphism (RFLP) (ARADHYA et al, 1999), Amplified Fragment Length Polymorphism (AFLP) markers (KIM et al, 2002;VAN DROOGENBROECK et al, 2002;RATCHADAPORN et al, 2007), PCR-RFLP ( VAN DROOGENBROECK et al, 2004), Inter-Simple Sequence Repeats (ISSR) (CARRASCO et al, 2009) and Simple Sequence Repeats (SSR) (OLIVEIRA et al, 2010a(OLIVEIRA et al, , 2010b. However, most of these studies are concerned with the diversity in germplasm collections without immediate commercial value for cultivar release.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of papaya genotypes using AFLP markers

et al. 2011

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-Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes, and the improved germplasm (CMF041) and landrace (CMF233) the most distant. The main papaya cultivars commercially grown in Brazil, as well as four inbred lines and three improved germplasm, were clustered together, however, were not grouped in the same branch. The genetic distance between the Sunrise and Golden cultivars was 0.329, and even arising from mutation and selection within the Sunrise variety, the Golden stores considerable genetic variability. Additional variability was observed in the inbred lines derived from papaya breeding program at Embrapa Cassava and Fruits. Index terms: papaya breeding, genetic resources, genetic diversity, Carica papaya L.. CARACTERIZAÇÃO MOLECULAR DE GENÓTIPOS DE MAMOEIRO COM USO DE MARCADORES DO TIPO AFLPRESUMO -Em virtude da baixa variabilidade genética relatada nos plantios comerciais de mamoeiro (Carica papaya L.), o objetivo deste trabalho foi analisar a divergência genética de 32 genótipos incluindo cultivares, variedades locais, linhagens e germoplasma melhorado, utilizando a técnica de AFLP (Amplified Fragment Length Polymorphism). A matriz de distâncias genéticas foi obtida com a distância de Nei e Li, e o agrupamento foi realizado utilizando o método UPGMA (unweighted pair-method with arithmetic mean). Utilizaram-se 11 combinações de iniciadores com corte das enzimas EcoRI / MseI. Foram geradas 383 bandas polimórficas, com média de 34,8 por combinação de iniciadores. O agrupamento dos dados permitiu a formação de cinco grupos, sendo a cultivar Sunrise e a linhagem CMF-L30-08 os genótipos mais relacionados, e CMF041 (germoplasma melhorado) e CMF233 (variedade local), os mais dissimilares. As cultivares de mamoeiro mais cultivadas no Brasil, bem como quatro linhagens e três acessos de germoplasma melhorado foram agrupadas, porém não no mesmo ramo. Mesmo sendo oriunda de mutação e seleção dentro de Sunrise, a cultivar Golden ainda possui considerável variabilidade genética, uma vez que a distância genética entre estas cultivares foi de 0,329. Variabilidade adicional foi observada nas linhagens do programa de melhoramento. Termos para indexação: melhoramento, recursos genéticos, diversidade genética, Carica papaya L..Rev. Bras.

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Section: Gs Xy = 2n Xy N X + N Ysupporting

confidence: 94%

Section: Cluster Analysismentioning

confidence: 40%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of papaya genotypes using AFLP markers

et al. 2011

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“…The nature of the data set seems suitable for parsimony (Hadrys et a!., 1992), but this is not often used. Stiles et al (1993) found different distance measures gave a similar result, consistent with tradi-…”

Section: Discussionsupporting

confidence: 80%

“…Despite this, several studies have used them successfully in phylogenetic analysis (Demeke et a!., 1992;Stiles et al, 1993;Badaracco et at., 1995;Stammers et al, 1995;Comincini et a!., 1996;Millan et a!., 1996). A consensus opinion seems to be that RAPDs are reliable for intraspecific variation, but become unreliable as phylogenetic distance increases (Grosberg et a!., 1996;Rieseberg, 1996).…”

Section: Introductionmentioning

confidence: 99%

Variability of the bushcricket Ephippiger ephippiger: RAPDs and song races

et al. 1997

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The tettigoniid bushcricket, Ephippiger, shows extensive variability in morphology and behaviour in Western Europe. Several species and subspecies have been described, but many may only represent local forms of uncertain origin. Within Ephippiger ephippiger, patterns of variation differ between morphology (upon which most taxonomic forms are defined) and behaviour. There is sufficient variation in a sexual signal (calling song) within the species that 'song races' have been described, which show strong premating isolation from each other. Here RAPD markers (138 fragments from 10 primers) have been used to examine the extent and pattern of genetic differentiation within the species and between two congeneric species.Ephippiger ephippiger is clearly distinguished from its relatives. High genetic similarity has been found within E. ephippiger, Nei and Li's S averaging 0.67. Using both distance and parsimony methods resolves the relationships of the song races and morphological forms of E. ephippiger with the exception of one population. The song races represent the deepest division within the species, but the lack of concordance in different traits suggests that a complex pattern of subspecific variation is found within this species.

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