Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses’ Specific Nucleic Acids

Krejčová, Ludmila; Húska, Dalibor; Hynek, David; Kopel, Pavel; Adam, Vojtěch; Hubálek, Jaromír; Trnková, Libuše; Kizek, René

doi:10.1016/s1452-3981(23)14051-x

Cited by 26 publications

(1 citation statement)

References 70 publications

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“…This binary assay offers rapid and straightforward virus detection at the expense of finite quantification. Krejcova et al used electrochemical detection to identify the H1N1, H3N2, and H5N1 influenza subtypes [ 102 ]. They used probe-conjugated paramagnetic microparticles to capture and isolate three influenza oligonucleotides.…”

Section: Viruses Detected Using Microparticlesmentioning

confidence: 99%

Microparticle-Based Detection of Viruses

Khanthaphixay

Yoon

2023

Biosensors

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Surveillance of viral pathogens in both point-of-care and clinical settings is imperative to preventing the widespread propagation of disease—undetected viral outbreaks can pose dire health risks on a large scale. Thus, portable, accessible, and reliable biosensors are necessary for proactive measures. Polymeric microparticles have recently gained popularity for their size, surface area, and versatility, which make them ideal biosensing tools. This review cataloged recent investigations on polymeric microparticle-based detection platforms across eight virus families. These microparticles were used as labels for detection (often with fluorescent microparticles) and for capturing viruses for isolation or purification (often with magnetic microparticles). We also categorized all methods by the characteristics, materials, conjugated receptors, and size of microparticles. Current approaches were compared, addressing strengths and weaknesses in the context of virus detection. In-depth analyses were conducted for each virus family, categorizing whether the polymeric microparticles were used as labels, for capturing, or both. We also summarized the types of receptors conjugated to polymeric microparticles for each virus family.

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Section: Viruses Detected Using Microparticlesmentioning

confidence: 99%

Microparticle-Based Detection of Viruses

Khanthaphixay

Yoon

2023

Biosensors

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Quantum dot as probe for disease diagnosis and monitoring

Mukherjee

Shim

Song

2015

Biotechnology Journal

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Semiconductor quantum dots (QD) possess unique optical and electric properties like size-tunable light emission, narrow emission range, high brightness and photostability. Recent research advances have minimized the toxicity of QDs and they are successfully used in in vitro and in vivo imaging. Encapsulation of QDs into polymeric nanoparticles and linking them with targeting ligands enabled the detection of tumors and cancer cells in vivo. QD-antibody conjugates were successfully used in monitoring and diagnosis of HIV and myocardial infarction. Application of near infrared (NIR) QDs was found to minimize the absorption and scattering of light by native tissues thus rendering them suitable in deep tissue analysis. Aggregation and endosomal sequestration of QDs pose major challenges for the effective delivery of QDs to the cell cytosol. Toxicity minimization and effective delivery strategies may further increase their suitability for utilization in disease diagnosis. New synthesis of QDs may provide new types of bioconjugates of QDs to biomolecules, which leads to a variety of applications to many challenged research areas. QDs with narrow emission wavelength ranges are very suitable for monitoring multiple cellular targets simultaneously, and still remain the best known probes for imaging as an alternative to traditional fluorophores in disease diagnosis.

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Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses’ Specific Nucleic Acids

Cited by 26 publications

References 70 publications

Microparticle-Based Detection of Viruses

Microparticle-Based Detection of Viruses

Quantum dot as probe for disease diagnosis and monitoring

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

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