Using Odd-Alkanes as a Carbon Source to Increase the Content of Nutritionally Important Fatty Acids inCandida krusei, Trichosporon cutaneum,andYarrowia lipolytica

Abstract

We investigated the possibility of utilizing unusual carbon sources by three yeast strains: Candida krusei DBM 2136, Trichosporon cutaneum CCY 30-5-10, and Yarrowia lipolytica CCY 30-26-36. These strains are characterized by high biomass yield, ability to accumulate high amounts of lipids, and their potential as producers of dietetically important fatty acids. The aim of this work was the production of nutritionally important fatty acids by utilization of n-alkanes with an odd number of carbon atoms, alone and in combination with glucose and subsequent analysis of microbial lipids accumulation and fatty acid profile. All three yeast strains were able to grow and produce high amounts of the fatty acids of interest. Yarrowia lipolytica was found as the most suitable strain for the growth on n-alkanes (n-pentadecane and n-heptadecane) as the only source of carbon. The addition of biosurfactants rhamnolipids into the cultivation increased the ratio of heptadecenoic acid (up to 17.9% of total FAs in Y. lipolytica CCY 30-26-36, 14.9% in T. cutaneum CCY 30-5-10, and 17.5% in C. krusei DBM 2136) and the total biomass yield. The results show that, by manipulation of the initial cultivation conditions, the ratio of important fatty acids may be increased.

Use of Non-Conventional Yeast Yarrowia lipolytica in Treatment or Upgradation of Hydrophobic Industry Wastes

“…The yeast Y. lipolytica has been intensively studied in view of its highly efficient degradation of hydrophobic substrates such as alkanes [13]. Matatkova et al [115] conducted research to evaluate the ability of Y. lipolytica CCY 30-26-36 strain to grow in media containing glucose with odd n-alkanes (pentadecane/heptadecane) or odd alkanes as the only source of carbon. Microbial oil from yeast biomass contained about 80% of unsaturated fatty acids.…”

Rhamnolipids as a Tool for Eradication of Trichosporon cutaneum Biofilm

“…The mechanism of action is believed to be based on the solubilization of cell membranes [ 1 , 3 ]. Therefore, rhamnolipids are intensively studied as potential antimicrobial and antibiofilm substances to be used for the treatment of surfaces against drug-resistant pathogens, the development of new pharmaceuticals [ 4 ] or the modulation of hydrophobic substrates intake [ 5 ].…”

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

“…DCAs are important platform chemicals and building blocks for biodegradable polymers (Ngo et al 2006). It is also possible to employ n-alkanes as inexpensive substrates for biotransformation into microbial lipids by T. cutaneum (Matakova et al 2017). This work determines the ability of the Trichosporon domesticum strain isolated from kefir to accumulate intracellular lipids in media with DPW containing glucose, lactose, and glycerol as carbon sources.…”