Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Abstract

This study determines the ability of an isolated Trichosporon domesticum yeast strain to accumulate intracellular lipids in media with deproteinated potato wastewater (DPW) containing various carbon sources. The yeast strain was isolated from kefir and identified by internal transcribed spacer (ITS) region sequence analysis. The sequence was deposited in GenBank under accession number MH094668, and the strain was deposited in Polish Collection of Microorganisms as T. domesticum PCM 2960. DPW is an inexpensive and valuable source of nitrogen, potassium, phosphorus, and other elements in yeast cultures. DPW supplemented with glucose medium was most effective at stimulating lipid biosynthesis by T. domesticum PCM 2960 and bioreactor incubation resulted in a final lipid yield of 4.8 g L −1. The lipids of the T. domesticum PCM 2960 biomass were characterized by high contents of linoleic acid (Δ9,12 C18:2), oleic acid (Δ9 C18:1), palmitic acid (C16:0), and α-linolenic acid (Δ9,12,15 C18:3). Theoretical calculations for biodiesel properties showed that the methylated esters of lipids from T. domesticum PCM 2960 biomass cannot be used as a biodiesel in diesel engines. Additionally, the ability to produce biofilm as one criterion for pathogenicity was tested. The ability for biofilm formation by the isolated strain was low. This study provides a promising solution for the more economical production of microbial lipids with DPW.

Valorization of Deproteinated Potato Juice Water into β-Glucan Preparation of C. utilis Origin: Comparative Study of Preparations Obtained by Two Isolation Methods

“…According to trends analysis in global potato starch industry the market reached app. 3.6 mln tons in 2017 and the forecast indicate an increase in potato starch production [15,17]. In accordance with calculations [15] the global potato starch industry generated app.…”

“…Our previous studies stated the important increase in the content of discussed functional polysaccharide in cells of C. utilis cultivated on food-agricultural waste-deproteinated potato juice water (DPJW) with the addition of glycerol [10,13]. Management of substantial quantity of DPJW constitutes a problem for the potato starch industry [14][15][16]. The waste arises after deproteinization of potato juice generated through potato tubers disintegration during starch separation.…”

“…The waste arises after deproteinization of potato juice generated through potato tubers disintegration during starch separation. Discussed by-product is also named as deproteinated potato wastewater in literature [15]. According to trends analysis in global potato starch industry the market reached app.…”

Valorisation of potato wastes

“…Raigond et al () studied the possibility of converting starch from waste potatoes and peel wastes in high valuable nanocrystals, which can be further modified as desired. Gientka et al () proposed the valorisation of potato wastes using their deproteinated wastewater as a sustainable nitrogen source in Trichosporon domesticum yeast lipids biosynthesis. Isolated protein products were used for animal feed.…”

“…Potato leaves are additional disposals of potato processing (Anjali Krishna et al , ). Also from the starch processing industry, large amount of residues are generated as wastewaters, where important amounts of gelling or bioactive compounds are contained and could be easily recovered (Smerilli et al , ; Torres et al , ; Gientka et al , ).…”