Using multiple endpoints to assess the toxicity of cadmium and cobalt for chlorophycean Raphidocelis subcapitata

Reis, Larissa Luiza dos; Alho, Lays de Oliveira Gonçalves; Abreu, Cínthia Bruno de; Melão, Maria da Graça Gama

doi:10.1016/j.ecoenv.2020.111628

Cited by 21 publications

(11 citation statements)

References 85 publications

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“…We observed that the concentration allowed for Cr by legislation (50 µg Cr L −1 , CONAMA 2005) for the protection of aquatic communities was su cient to affect physiological aspects in planktonic species. However, extrapolation and interpretation of the toxic effects of metals obtained from controlled laboratory conditions to the natural environment must be performed with caution (Reis et al 2021). Considering the toxic effects for chlorophycea and the tropical species C. silvestrii, the present work contributes to a better understanding of the contamination pathways and effects of the metal on aquatic biota.…”

Section: Discussionmentioning

confidence: 97%

Chromium toxicity to planktonic organisms: comparison between different routes of exposure to the metal

Vieira

Rodgher

Haneda

et al. 2022

Preprint

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Given the importance of metals in the contamination of aquatic ecosystems, studies are needed to assess the effects of these contaminants on aquatic organisms through different contamination pathways. Chromium (Cr) is a metal widely used in various industrial processes, considered a priority pollutant in several countries. In the present study, ecotoxicological tests were carried out with the microalga Chlorella vulgaris, evaluating the effects of Cr on cell density, pigment concentration, and biochemical composition. Additionally, the effects of chromium exposure on cladocerans (Ceriodaphnia dubia and Ceriodaphnia silvestrii) were investigated through three contamination routes: contaminated medium, contaminated food (C. vulgaris), and the combination of both situations (contaminated medium and food). The toxicity tests with cladocerans evaluated the metal effects on survival, number of newborn produced by female, and feeding activity. The results obtained demonstrated that 48 µg L-1 total dissolved Cr reduced cell density and increased protein, carbohydrate, and lipid content in C. vulgaris. The increase in the biochemical composition of microalgae upon exposure to Cr may have contributed to the non-observation of chronic toxic effects on daphnids in the treatment with contaminated food. A decrease of 31% and 26% in the number of C. silvestrii offspring was observed for the treatment with contaminated medium (35 µg L-1 total dissolved Cr) and associated treatment (35 µg L-1 total dissolved Cr and 3 × 10-14 g Cr cells-1), respectively. The importance of the Cr poisoning pathways and the difference in cladoceran species’ sensitivity to the metal were addressed.

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Section: Discussionmentioning

confidence: 97%

Chromium toxicity to planktonic organisms: comparison between different routes of exposure to the metal

Vieira

Rodgher

Haneda

et al. 2022

Preprint

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“…Moreover, as a vital physiological process of microalgae, photosynthesis has important functions for aquatic ecosystems, which can provide material and energy sources for other organisms. As a result, microalgal photosynthesis is crucial for the normal primary production of the aquatic ecosystem [ 8 ]. However, many studies have reported that heavy metals have toxic effects on the photosynthesis of microalgae [ 1 , 8 , 9 , 10 , 11 , 12 , 13 ] by inhibiting the absorption of light energy, the transmission of photosynthetic electrons, and the conversion of photosynthetic energy [ 14 , 15 , 16 ], which will seriously affect the primary productivity of aquatic ecosystems and pose potential risks to the aquatic environment in severe cases.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, microalgal photosynthesis is crucial for the normal primary production of the aquatic ecosystem [ 8 ]. However, many studies have reported that heavy metals have toxic effects on the photosynthesis of microalgae [ 1 , 8 , 9 , 10 , 11 , 12 , 13 ] by inhibiting the absorption of light energy, the transmission of photosynthetic electrons, and the conversion of photosynthetic energy [ 14 , 15 , 16 ], which will seriously affect the primary productivity of aquatic ecosystems and pose potential risks to the aquatic environment in severe cases. Therefore, rapid and sensitive detection of the toxicity of the heavy metals in water to the photosynthesis of microalgae is of great significance for evaluating the impacts of heavy metals on aquatic ecosystems and predicting their potential environmental risks.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Response Index Selection for Rapid Assessment of Heavy Metals Toxicity to the Photosynthesis of Chlorella pyrenoidosa Based on Rapid Chlorophyll Fluorescence Induction Kinetics

Gan

Yin

Zhao

et al. 2023

Toxics

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Heavy metals as toxic pollutants have important impacts on the photosynthesis of microalgae, thus seriously threatening the normal material circulation and energy flow of the aquatic ecosystem. In order to rapidly and sensitively detect the toxicity of heavy metals to microalgal photosynthesis, in this study, the effects of four typical toxic heavy metals, chromium (Cr(VI)), cadmium (Cd), mercury (Hg), and copper (Cu), on nine photosynthetic fluorescence parameters (φPo, ΨEo, φEo, δRo, ΨRo, φRo, FV/FO, PIABS, and Sm) derived from the chlorophyll fluorescence rise kinetics (OJIP) curve of microalga Chlorella pyrenoidosa, were investigated based on the chlorophyll fluorescence induction kinetics technique. By analyzing the change trends of each parameter with the concentrations of the four heavy metals, we found that compared with other parameters, φPo (maximum photochemical quantum yield of photosystem II), FV/FO (photochemical parameter of photosystem II), PIABS (photosynthetic performance index), and Sm (normalized area of the OJIP curve) demonstrated the same monotonic change characteristics with an increase in concentration of each heavy metal, indicating that these four parameters could be used as response indexes to quantitatively detect the toxicity of heavy metals. By further comparing the response performances of φPo, FV/FO, PIABS, and Sm to Cr(VI), Cd, Hg, and Cu, the results indicated that whether it was analyzed from the lowest observed effect concentration (LOEC), the influence degree by equal concentration of heavy metal, the 10% effective concentration (EC10), or the median effective concentration (EC50), the response sensitivities of PIABS to each heavy metal were all significantly superior to those of φRo, FV/FO, and Sm. Thus, PIABS was the most suitable response index for sensitive detection of heavy metals toxicity. Using PIABS as a response index to compare the toxicity of Cr(VI), Cd, Hg, and Cu to C. pyrenoidosa photosynthesis within 4 h by EC50 values, the results indicated that Hg was the most toxic, while Cr(VI) toxicity was the lowest. This study provides a sensitive response index for rapidly detecting the toxicity of heavy metals to microalgae based on the chlorophyll fluorescence induction kinetics technique.

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“…Copper (Cu) is an essential metal used in numerous physiological processes but becomes toxic at higher concentrations ( Gaetke and Chow, 2003 ; Rico et al., 2013 ) leading to DNA damage ( Mittler, 2002 ; Knauert and Knauer, 2008 ). Cobalt (Co) is also an essential metal and a constituent of vitamin B12 required for cellular growth ( Adam and Garnier-Laplace, 2003 ; dos Reis et al., 2021 ). In presence of high concentrations of Co(II), the syntheses of chlorophyll a and chlorophyll b ( El-Sheekh et al., 2003 ; Fathi et al., 2008 ) and RNA ( Nowicka, 2022 ) is disturbed.…”

Section: Introductionmentioning

confidence: 99%

Molecular changes in phenolic compounds in Euglena gracilis cells grown under metal stress

Bernard

Guéguen

2023

Front. Plant Sci.

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Metal presence in the aquatic ecosystem has increased and diversified over the last decades due to anthropogenic sources. These contaminants cause abiotic stress on living organisms that lead to the production of oxidizing molecules. Phenolic compounds are part of the defense mechanisms countering metal toxicity. In this study, the production of phenolic compounds by Euglena gracilis under three different metal stressors (i.e. cadmium, copper, or cobalt) at sub-lethal concentration was assessed using an untargeted metabolomic approach by mass spectrometry combined with neuronal network analysis (i.e. Cytoscape). The metal stress had a greater impact on molecular diversity than on the number of phenolic compounds. The prevalence of sulfur- and nitrogen-rich phenolic compounds were found in Cd- and Cu-amended cultures. Together these results confirm the impact of metallic stress on phenolic compounds production, which could be utilized to assess the metal contamination in natural waters.

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Using multiple endpoints to assess the toxicity of cadmium and cobalt for chlorophycean Raphidocelis subcapitata

Cited by 21 publications

References 85 publications

Chromium toxicity to planktonic organisms: comparison between different routes of exposure to the metal

Chromium toxicity to planktonic organisms: comparison between different routes of exposure to the metal

A Sensitive Response Index Selection for Rapid Assessment of Heavy Metals Toxicity to the Photosynthesis of Chlorella pyrenoidosa Based on Rapid Chlorophyll Fluorescence Induction Kinetics

Molecular changes in phenolic compounds in Euglena gracilis cells grown under metal stress

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