A Sensitive Response Index Selection for Rapid Assessment of Heavy Metals Toxicity to the Photosynthesis of Chlorella pyrenoidosa Based on Rapid Chlorophyll Fluorescence Induction Kinetics

Gan, Tingting; Yin, Guisheng; Zhao, Nanjing; Tan, Xiaoxuan; Wang, Y.

doi:10.3390/toxics11050468

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…The drop in TR O /ABS indicates that the light use efficiency of PSII RCs is lowered by Cd stress [39,65]. PI ABS , a comprehensive performance index of PSII [25,58,66], is a more sensitive response index than TR O /ABS in quantitatively detecting the toxicity of heavy metals [67]. In this study, PI ABS was more sensitive than TR O /ABS to Cd stress.…”

Section: Discussionmentioning

confidence: 58%

Exploration of the Effects of Cadmium Stress on Photosynthesis in Oenanthe javanica (Blume) DC.

Zhou,

Xu,

et al. 2024

Toxics

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Cadmium ion (Cd2+) stress is a major abiotic stressor affecting plant photosynthesis. However, the impact of sustained high-concentration Cd stress on the photosynthetic electron transport chain of aquatic plants is currently unclear. Here, prompt fluorescence (PF), delayed fluorescence (DF), and P700 signals were simultaneously measured to investigate the effect of Cd stress on photosynthesis in water dropwort [Oenanthe javanica (Blume) DC.]. We aimed to elucidate how Cd stress continuously affects the electron transport chain in this species. The PF analysis showed that with prolonged Cd stress, the FJ, FI and FP steadily decreased, accompanied by a positive shift in the K-band and L-band. Moreover, JIP-test parameters, including TRO/ABS, ABS/CSO, TRO/CSO and PIABS, were significantly reduced. The P700 signals showed that exposure to Cd stress hindered both the fast decrease and slow increase phases of the MR transient, ultimately resulting in a gradual reduction in both VPSI and VPSII − PSI. The DF analysis showed a gradual decrease in the I1 and I2 values as the duration of stress from Cd increased. The above results suggested that Cd stress affected the photosynthetic electron transport in water dropwort by influencing the amount of active PSII and PSI, primarily affecting PSII RCs in the early to mid-stages and PSI reductive activity in the later stage.

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Section: Discussionmentioning

confidence: 58%

Exploration of the Effects of Cadmium Stress on Photosynthesis in Oenanthe javanica (Blume) DC.

Zhou,

Xu,

et al. 2024

Toxics

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“…Root cutting induced changes in OJIP curve of A. ordosica leaves Rapid ChlF can be an effective tool for monitoring the photosynthetic physiological status of plants under various environmental stresses, such as salt stress (Zushi and Matsuzoe, 2017), drought stress (Erdal and Ekmekci, 2023), and heavy metal stress (Gan et al, 2023), among others, in several plant species. OJIP transient detection technology, as a non-destructive, simple, and fast detection method, is widely used to evaluate the degree of damage to photosynthetic organs under various environmental stresses (Kalaji et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll a fluorescence as a tool to monitor physiological status in the leaves of Artemisia ordosica under root cutting conditions

Liu,

Gong,

Pei

et al. 2024

Front. Plant Sci.

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BackgroundRoot cutting caused by underground coal mining subsidence is among the leading causes of plant damage in western China. Detection of root cutting stress is of great importance in evaluating the degree of plant damage and changes in physiological conditions in underground coal mining disturbance conditions.MethodsThe present study assessed the use of chlorophyll fluorescence OJIP transient data to evaluate the disturbance characteristics of root cutting stress on leaf photosynthetic mechanisms in the typical shrub Artemisia ordosica Krasch. Different root cutting ratios (10%, 20%, 30%, 50%, 75%, and 100%) were established on the roots of A. ordosica in the field, and the OJIP transient and JIP parameters of the leaves were measured.ResultsThe overall OJIP curves and each OJIP step in leaves decreased as the root cutting ratio increased, but the impact was relatively small for root cutting ratios of less than 30%. Through the analysis of JIP parameters and the established energy pipeline model, it was found that the energy capture efficiency and electron transfer efficiency of photosystem II decreased as the root cutting ratio increased. Therefore, we also inferred that the threshold for the plant root cutting ratio at which leaf photosynthetic mechanisms begin to change is 30–50%.ConclusionThese results indicate that OJIP transient analysis can serve as a non-destructive, rapid technique for detecting plant root cutting stress in coal mining subsidence areas, which is of great value for non-destructive monitoring of plant root damage.

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“…Furthermore, toxicological studies also incorporated additional test endpoints, such as chlorophyll content [ 10 , 13 , 14 ], enzyme activities [ 15 , 16 ], colony size [ 17 , 18 ] and root length [ 19 , 20 ]. More recently, as an emerging non-invasive technique, the chlorophyll fluorescence (ChlF) induction method has also been increasingly utilized in various plant- and algae-based ecotoxicological studies [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Phytotoxicity of Metallic Elements on Duckweed Lemna gibba L. Using Growth- and Chlorophyll Fluorescence Induction-Based Endpoints

Irfan,

Mészáros,

Szabó

et al. 2024

Plants

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In this study, we exposed a commonly used duckweed species—Lemna gibba L.—to twelve environmentally relevant metals and metalloids under laboratory conditions. The phytotoxic effects were evaluated in a multi-well-plate-based experimental setup by means of the chlorophyll fluorescence imaging method. This technique allowed the simultaneous measuring of the growth and photosynthetic parameters in the same samples. The inhibition of relative growth rates (based on frond number and area) and photochemical efficiency (Fv/Fo and Y(II)) were both calculated from the obtained chlorophyll fluorescence images. In the applied test system, growth-inhibition-based phytotoxicity endpoints proved to be more sensitive than chlorophyll-fluorescence-based ones. Frond area growth inhibition was the most responsive parameter with a median EC50 of 1.75 mg L−1, while Fv/Fo, the more responsive chlorophyll-fluorescence-based endpoint, resulted in a 5.34 mg L−1 median EC50 for the tested metals. Ag (EC50 0.005–1.27 mg L−1), Hg (EC50 0.24–4.87 mg L−1) and Cu (EC50 0.37–1.86 mg L−1) were the most toxic elements among the tested ones, while As(V) (EC50 47.15–132.18 mg L−1), Cr(III) (EC50 6.22–19.92 mg L−1), Se(VI) (EC50 1.73–10.39 mg L−1) and Zn (EC50 3.88–350.56 mg L−1) were the least toxic ones. The results highlighted that multi-well-plate-based duckweed phytotoxicity assays may reduce space, time and sample volume requirements compared to the standard duckweed growth inhibition tests. These benefits, however, come with lowered test sensitivity. Our multi-well-plate-based test setup resulted in considerably higher median EC50 (3.21 mg L−1) for frond-number-based growth inhibition than the 0.683 mg L−1 median EC50 derived from corresponding data from the literature with standardized Lemna-tests. Under strong acute phytotoxicity, frond parts with impaired photochemical functionality may become undetectable by chlorophyll fluorometers. Consequently, the plant parts that are still detectable display a virtually higher average photosynthetic performance, leading to an underestimation of phytotoxicity. Nevertheless, multi-well-plate-based duckweed phytotoxicity assays, combined with chlorophyll fluorescence imaging, offer definite advantages in the rapid screening of large sample series or multiple species/clones. As chlorophyll fluorescence images provide information both on the photochemical performance of the test plants and their morphology, a joint analysis of the two endpoint groups is recommended in multi-well-plate-based duckweed phytotoxicity assays to maximize the information gained from the tests.

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A Sensitive Response Index Selection for Rapid Assessment of Heavy Metals Toxicity to the Photosynthesis of Chlorella pyrenoidosa Based on Rapid Chlorophyll Fluorescence Induction Kinetics

Cited by 5 publications

References 41 publications

Exploration of the Effects of Cadmium Stress on Photosynthesis in Oenanthe javanica (Blume) DC.

Exploration of the Effects of Cadmium Stress on Photosynthesis in Oenanthe javanica (Blume) DC.

Chlorophyll a fluorescence as a tool to monitor physiological status in the leaves of Artemisia ordosica under root cutting conditions

Comparative Phytotoxicity of Metallic Elements on Duckweed Lemna gibba L. Using Growth- and Chlorophyll Fluorescence Induction-Based Endpoints

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