Using metabolomics to develop precision medicine strategies to treat nonalcoholic steatohepatitis

Changes in the serum metabolomic profiles of subjects with NAFLD in response to n-3 PUFAs and phytosterol ester: a double-blind randomized controlled trial

“…Metabolomics allows the exploration of the complex relationship between nutrition and metabolism and is considered an ideal choice for the investigation of physiological functions of beneficial compounds at the molecular level. 26 Serum contains hundreds of small molecules, some of which may serve as biomarkers for monitoring pathological states and responses to dietary interventions. 27,28 In the present study, to explore the serum metabolic profile of NAFLD subjects treated with the combination of PSE and n-3 PUFAs, the NAFLD subjects in three study groups were given PSE, n-3 PUFAs or a combination of PSE and n-3 PUFAs daily for 12 continuous weeks, and serum metabolites were analysed using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS E [E represents collision energy]) on the basis of a previous study.…”