Changes in the serum metabolomic profiles of subjects with NAFLD in response to n-3 PUFAs and phytosterol ester: a double-blind randomized controlled trial

XianBin, Ding; Xu, Yinfei; Nie, Pan; Zhong, Lingyue; Feng, Lihua; Qi, Guijun; Liu, Song

doi:10.1039/d1fo03921k

Cited by 8 publications

(6 citation statements)

References 53 publications

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“…In the present study, we observed that a high dose of n -3 PUFA and n -6 PUFA supplementation increased the level of PC (32:3), and decreased the levels of PC (40:9), PC (42:10), and Cer-18:0. A previous study found that fish oil supplementation increased serum PC (35:5) and PC (36:5) levels and decreased PC (36:6) and PC (38:1) levels in patients with nonalcoholic fatty liver disease (NAFLD) [ 41 ]. Moreover, the C20:4n-6 enriched diet significantly decreased the hepatic levels of PC containing C18:2n-6 in mice compared with the control diet [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of n-3 and n-6 Polyunsaturated Fatty Acids on Placental and Embryonic Growth and Development in Diabetic Pregnant Mice

Li,

Chen,

Liu

et al. 2024

Nutrients

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The present study aimed to investigate the differential effects of n-3 and n-6 polyunsaturated fatty acids (PUFAs) on placental and embryonic development. Pregnant mice were assigned to five groups: healthy control (HC), diabetes mellitus control (DMC), diabetes + low-dose n-3 PUFA (Ln-3), diabetes + high-dose n-3 PUFA (Hn-3), and diabetes + n-6 PUFA (n-6). On E12.5d, the Hn-3 group, but not the n-6 group, had a higher placenta weight. The weight ratio of embryo to placenta in the n-6 group was significantly lower than in the Hn-3 group but higher than in the DMC group. The Hn-3 group had significantly higher protein levels of VEGF, IGF-1, and IGFBP3, while the n-6 group had lower VEGF than the DMC group. Compared with the DMC group, embryonic Cer-16:0 was significantly higher in the Hn-3 group, while embryonic PC (36:6), PC (38:7), and PE (40:7) were significantly lower in the n-6 group. The embryo and placenta weights were positively correlated with placental VEGF, IGFBP3, and embryonic Cer-16:0, and they were negatively correlated with embryonic PC (36:6) and PE (40:7). The weight ratio of embryo to placenta was negatively correlated with embryonic PC (36:6). In addition, embryonic Cer-16:0 was positively correlated with placental VEGF and IGFBP3. In conclusion, n-3 PUFA and n-6 PUFA improved placental and embryonic growth through different mechanisms.

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Section: Discussionmentioning

confidence: 99%

Differential Effects of n-3 and n-6 Polyunsaturated Fatty Acids on Placental and Embryonic Growth and Development in Diabetic Pregnant Mice

Li,

Chen,

Liu

et al. 2024

Nutrients

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“…For example, statins can induce various adverse events including muscle disorders, liver dysfunction, kidney injury, and myocardial infarction (Cai et al, 2021). Some functional food, such as chickpeas (Mirizzi et al, 2019), wheat germ (Salehi‐Sahlabadi et al, 2022), and n‐3 polyunsaturated fatty acids (Ding et al, 2022) are used to prevent hepatic fat accumulation, thereby providing a potential way to ameliorate NAFLD. In this study, Bio‐A, one of the isoflavones in chickpea, reduced the intracellular TG content, inhibited ADRP expression, increased the expression of autophagosome formation biomarkers Beclin 1, LC3‐II, and ULK‐1 phosphorylation, and decreased the expression of p62, an autophagic flux biomarker.…”

Section: Discussionmentioning

confidence: 99%

Biochanin A ameliorated oleate‐induced steatosis in HepG2 cells by activating the SIRT3/AMPK/ULK‐1 signaling pathway

Wang

Liu

Yang

et al. 2022

Journal of Food Biochemistry

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Biochanin A (Bio‐A), an isoflavone abundant in chickpeas, possesses hypoglycemic, hypolipidemic, and anti‐inflammatory effects. However, whether Bio‐A has antihepatosteatosis effect remains unclear. This study aimed to evaluate the antihepatosteatosis effect of Bio‐A on oleate (OA)‐treated hepatocytes, and explore the underlying mechanism. When incubated with OA for 24 h, HepG2 cells were treated with various concentrations of Bio‐A for 24 h to obtain an optimal antihepatosteatosis dose. HepG2 cells were treated with the AMP‐activated protein kinase (AMPK) inhibitor Compound C, or the sirtuin‐3 (SIRT3) inhibitor 3‐TYP, and incubated with 50 μM Bio‐A. The results indicated that 12.6% of lipid content, particularly 11.0% of triglyceride content, and the expression of adipocyte differentiation‐related protein were significantly decreased in Bio‐A‐treated hepatosteatosis cells, followed by an increase in the expression of Beclin 1, phosphorylation of Unc‐51‐like kinase 1 (ULK‐1), the microtubule‐associated protein 1 light chain 3 (LC3)‐II/LC3‐I ratio, and a decrease in expression of p62. The results indicated that Bio‐A upregulated autophagosome formation and autophagy flux. In addition, Bio‐A increased SIRT3 expression and AMPK phosphorylation in OA‐treated HepG2 cells. Blockade of AMPK and SIRT3 blocked the antihepatosteatosis effect and ULK‐1 activation by Bio‐A. AMPK inhibition did not eliminate the activation of SIRT3 by Bio‐A. AutoDock analysis demonstrated that interaction might exist between Bio‐A and SIRT3. In conclusion, Bio‐A reduced fat accumulation in OA‐treated HepG2 cells by activating SIRT3/AMPK/ULK‐1‐mediated autophagy. The findings provide a theoretical basis for the effect of Bio‐A on hepatic steatosis‐related diseases. Practical applications This study highlights the antihepatosteatosis effects of biochanin A (Bio‐A) on oleate (OA)‐treated hepatocytes. Bio‐A, one of the isoflavones in Cicer arietinum Linn., possesses multiple bioactivities such as antiobesity, anti‐inflammation, and hypoglycemic and hypolipidemic effects. This study provides a new application of Bio‐A to treat hepatic steatosis, and revealed the underlying mechanism of Bio‐A involved in the activation of the SIRT3/AMPK/ULK‐1‐mediated autophagy. The findings provide a theoretical basis for the application of Bio‐A to hepatic steatosis‐related diseases.

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“…Untargeted metabolomic analysis was performed using a UPLC-ESI-Q-TOF-MS system. 22 The raw data was processed using Progenesis QI v2.3 software, which performed peak alignment, peak picking, and deconvolution. Metabolite identification was conducted by comparing the data with information from internet sources such as Metlin, LIPID MAPS, and the Human Metabolome database.…”

Section: Untargeted Metabolomic Analysismentioning

confidence: 99%

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Song,

Guo,

Sun

et al. 2024

Food Funct.

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Changes in the serum metabolomic profiles of subjects with NAFLD in response to n-3 PUFAs and phytosterol ester: a double-blind randomized controlled trial

Abstract: Nonalcoholic fatty liver disease (NAFLD) has become the most common cause of chronic liver disease and threatens human health worldwide. As shown in our previous study, cosupplementation with phytosterol ester...

Cited by 8 publications

References 53 publications

Differential Effects of n-3 and n-6 Polyunsaturated Fatty Acids on Placental and Embryonic Growth and Development in Diabetic Pregnant Mice

Differential Effects of n-3 and n-6 Polyunsaturated Fatty Acids on Placental and Embryonic Growth and Development in Diabetic Pregnant Mice

Biochanin A ameliorated oleate‐induced steatosis in HepG2 cells by activating the SIRT3/AMPK/ULK‐1 signaling pathway

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

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