Using Mass Spectrometry for Proteins

Vestling, Martha M.

doi:10.1021/ed080p122

Cited by 10 publications

(13 citation statements)

References 8 publications

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“…The traditional grooming model pairs a mentor with a protégé to enhance their possibility of success, while the networking model uses a non-hierarchical connections among a number of people to support the student [12]. During the training in proteomics, students had one-on-one meetings with the faculty director, Proteomics staff, and the project coordinator.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, the cost of the liquid chromatography and mass spectrometry instrumentation used in proteomics is prohibitive for most undergraduate institutions. Existing literature in chemical education provided individual example experiments in mass spectrometry [12–19] and proteomics [20–22]; these existing laboratory exercises could be combined with additional materials to develop a cohesive strategy for instruction. In addition to the development of a one semester curriculum, this element of the training would also need to support the transition from the undergraduate laboratory to a cancer research setting.…”

Section: Introductionmentioning

confidence: 99%

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An ET-CURE Pilot Project Supporting Undergraduate Training in Cancer Research, Emerging Technology, and Health Disparities

et al. 2012

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The National Cancer Institute’s Center to Reduce Cancer Health Disparities has created pilot training opportunities under the “Continuing Umbrella of Research Experiences” (CURE) program that focus on emerging technologies (ET). In this pilot project, an eighteen month cancer biology research internship was reinforced with: instruction in an emerging technology (proteomics), a transition from the undergraduate laboratory to a research setting, education in cancer health disparities, and community outreach activities. A major goal was to provide underrepresented undergraduates with hands-on research experiences that are rarely encountered at the undergraduate level, including mentoring, research presentations, and participation in local and national meetings. These opportunities provided education and career development for the undergraduates, and they have given each student the opportunity to transition from learning to sharing their knowledge and from being mentored to mentoring others. Here, we present the concepts, curriculum, infrastructure, and challenges for this training program along with evaluations by both the students and their mentors.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An ET-CURE Pilot Project Supporting Undergraduate Training in Cancer Research, Emerging Technology, and Health Disparities

et al. 2012

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“…John B. Fenn and Koichi Tanaka shared a Nobel prize in chemistry (2002) for the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), respectively [4]. Their contribution enabled scientist to identify and quantify proteins with a high level of precision.…”

Section: Techniques In Quantitative Proteomicsmentioning

confidence: 99%

A Quantitative Proteomics Approach to Clinical Research with Non-Traditional Samples

2016

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The proper handling of samples to be analyzed by mass spectrometry (MS) can guarantee excellent results and a greater depth of analysis when working in quantitative proteomics. This is critical when trying to assess non-traditional sources such as ear wax, saliva, vitreous humor, aqueous humor, tears, nipple aspirate fluid, breast milk/colostrum, cervical-vaginal fluid, nasal secretions, bronco-alveolar lavage fluid, and stools. We intend to provide the investigator with relevant aspects of quantitative proteomics and to recognize the most recent clinical research work conducted with atypical samples and analyzed by quantitative proteomics. Having as reference the most recent and different approaches used with non-traditional sources allows us to compare new strategies in the development of novel experimental models. On the other hand, these references help us to contribute significantly to the understanding of the proportions of proteins in different proteomes of clinical interest and may lead to potential advances in the emerging field of precision medicine.

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“…If a brief introduction to the operation principles is warranted, they can be explained using basic physics with which most students should be familiar (e.g., kinetic energy) or which will be introduced as part of the chemistry curriculum. Incorporation of mass spectrometry-based laboratory activities into the upper-division undergraduate laboratory environment is now well-established and widespread. − Albeit less widespread, there has also been a move to incorporate mass spectrometry in introductory-level laboratory work. , There have been several laboratory units developed incorporating mass spectrometry as a method for investigating isotopic abundances and patterns of various elements or molecules; while not all of these were aimed specifically at introductory courses, they are, perhaps, closest to mass spectrometry as a complement to the material typically taught in a general chemistry course. − Various overviews of mass spectrometry and its applications have previously been presented within the education community, including early overviews of mass spectrometry, − a history of mass spectrometry with a focus on its role in the Manhattan Project, a feature on the 2002 Nobel Prize in Chemistry summarizing the use of mass spectrometry for protein analysis, and a history of the application of mass spectrometry to the analysis of polymers . Mass spectrometry is already typically introduced to introductory/general chemistry students to varying degrees depending on the instructor and the textbook.…”

mentioning

confidence: 99%

“…9,10 There have been several laboratory units developed incorporating mass spectrometry as a method for investigating isotopic abundances and patterns of various elements or molecules; while not all of these were aimed specifically at introductory courses, they are, perhaps, closest to mass spectrometry as a complement to the material typically taught in a general chemistry course. 11−14 Various overviews of mass spectrometry and its applications have previously been presented within the education community, including early overviews of mass spectrometry, 15−17 a history of mass spectrometry with a focus on its role in the Manhattan Project, 18 a feature on the 2002 Nobel Prize in Chemistry summarizing the use of mass spectrometry for protein analysis, 19 and a history of the application of mass spectrometry to the analysis of polymers. 20 Mass spectrometry is already typically introduced to introductory/general chemistry students to varying degrees depending on the instructor and the textbook.…”

mentioning

confidence: 99%

Instructor’s Reference for Integrating Mass Spectrometry into the General Chemistry Classroom

Patrick

2020

J. Chem. Educ.

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Mass spectrometry is one of the few instrumental analysis techniques that can be relatively well-understood with physics/chemistry knowledge typical of an advanced high school or a beginning undergraduate student. This positions mass spectrometry well to be used as a bridge between abstract concepts typically set as learning goals in courses at those levels, practical problem sets based on realistic data graphs, and historical or applications case studies. Such case studies tie general chemistry learning goals in with areas of student interest, potential student career paths, and/or areas with which students might already be more familiar. The purpose of this work is to provide (1) an introduction to mass spectrometry, (2) a discussion of mass spectrometry applications which are both relevant to the general chemistry curriculum and likely of interest to the student population, and (3) examples of potential mass spectrometry-based exercises that could be integrated into classroom exercises or student assessment. Importantly, all three of these purposes are addressed at a level easily accessible for either instructors with little to no mass spectrometry background or for students in the target audience. An additional goal of the proposed activities is to encourage development of student skills in graph reading and analysis. Thus, this work’s aim is to serve as an instructor’s reference, bridging the gap between the field of mass spectrometry and the introductory or general chemistry classroom.

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Using Mass Spectrometry for Proteins

Cited by 10 publications

References 8 publications

An ET-CURE Pilot Project Supporting Undergraduate Training in Cancer Research, Emerging Technology, and Health Disparities

An ET-CURE Pilot Project Supporting Undergraduate Training in Cancer Research, Emerging Technology, and Health Disparities

A Quantitative Proteomics Approach to Clinical Research with Non-Traditional Samples

Instructor’s Reference for Integrating Mass Spectrometry into the General Chemistry Classroom

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