A Quantitative Proteomics Approach to Clinical Research with Non-Traditional Samples

Abstract

The proper handling of samples to be analyzed by mass spectrometry (MS) can guarantee excellent results and a greater depth of analysis when working in quantitative proteomics. This is critical when trying to assess non-traditional sources such as ear wax, saliva, vitreous humor, aqueous humor, tears, nipple aspirate fluid, breast milk/colostrum, cervical-vaginal fluid, nasal secretions, bronco-alveolar lavage fluid, and stools. We intend to provide the investigator with relevant aspects of quantitative proteomics and to recognize the most recent clinical research work conducted with atypical samples and analyzed by quantitative proteomics. Having as reference the most recent and different approaches used with non-traditional sources allows us to compare new strategies in the development of novel experimental models. On the other hand, these references help us to contribute significantly to the understanding of the proportions of proteins in different proteomes of clinical interest and may lead to potential advances in the emerging field of precision medicine.

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

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Mass spectrometry‐based tear proteomics for noninvasive biomarker discovery

“…The high resolution and sensitivity of state‐of‐the‐art MS instrumentations have improved the precision of proteomics profiles (Flores‐Morales & Iglesias‐Gato, 2017) and can dispense with pooled samples, the mandatory condition for an actually personalized medicine. In this context, the single‐tear analysis is standing out as an interesting, so‐called “nonconventional sample,” applicable to a single subject for obtaining personalized profiles (Gerner et al, 2020; Licier et al, 2016).…”

New frontiers on the molecular underpinnings of hypospadias according to severity

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