Using liquid crystals for the label-free detection of catalase at aqueous–LC interfaces

Hu, Qiongzheng; Jang, Chang‐Hyun

doi:10.1016/j.jbiotec.2011.11.010

Cited by 42 publications

(17 citation statements)

References 31 publications

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“…A second example involves the detection of catalase activity based on doping of the aldehyde dodecanal into 5CB. In brief, hydrogen peroxide was generated by the catalytic oxidation of dodecanal into dodecanoic acid, thus leading to homeotropic anchoring as the dodecanoic acid formed carboxylate anions at the aqueous interface [76]. Finally, we note that urease has been detected via changes in pH that were induced by the reaction product ammonia [77].…”

Section: Biomolecular Sensing At Lc-aqueous Interfacesmentioning

confidence: 99%

Chemical and biological sensing using liquid crystals

Carlton

Hunter

Miller

et al. 2013

Liquid Crystals Reviews

325

236

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The liquid crystalline state of matter arises from orientation-dependent, non-covalent interaction between molecules within condensed phases. Because the balance of intermolecular forces that underlies formation of liquid crystals is delicate, this state of matter can, in general, be easily perturbed by external stimuli (such as an electric field in a display). In this review, we present an overview of recent efforts that have focused on exploiting the responsiveness of liquid crystals as the basis of chemical and biological sensors. In this application of liquid crystals, the challenge is to design liquid crystalline systems that undergo changes in organization when perturbed by targeted chemical and biological species of interest. The approaches described below revolve around the design of interfaces that selectively bind targeted species, thus leading to surface-driven changes in the organization of the liquid crystals. Because liquid crystals possess anisotropic optical and dielectric properties, a range of different methods can be used to read out the changes in organization of liquid crystals that are caused by targeted chemical and biological species. This review focuses on principles for liquid crystal-based sensors that provide an optical output.

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Section: Biomolecular Sensing At Lc-aqueous Interfacesmentioning

confidence: 99%

Chemical and biological sensing using liquid crystals

Carlton

Hunter

Miller

et al. 2013

Liquid Crystals Reviews

325

236

View full text Add to dashboard Cite

show abstract

“…Due to its rapid orientational response on chemically functionalized surface, the nematic LC has served as an attractive medium to report chemical/biochemical events occurring at the interfacial biological membranes. [26][27][28][29][30][31][32][33][34][35][36][37][38] So, far uid biometric membrane systems supported on LCs have been coupled to the screening of specic protein binding event, 28,29,[31][32][33][34][35] DNA hybridization, [39][40][41][42] monitoring enzymatic reaction, 30,36,[43][44][45] and different pathogenic toxic detections. [46][47][48][49] For example, Abbott and his coworkers reported the specic interaction of immunoglobulin (IgG) antibody to the surface immobilized antigen resulting in a change in optical response of LCs.…”

Section: Introductionmentioning

confidence: 99%

Design of bio-molecular interfaces using liquid crystals demonstrating endotoxin interactions with bacterial cell wall components

2015

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Interaction of different bacterial cell membrane components such as, peptidoglycan (PG) and lipoteichoic acid (LTA) with bacterial endotoxin (LPS) shows diverse consequences on toxicity of gram negative bacteria in mammalian hosts, implying huge importance of studying this interaction for clinical understanding associated with gram negative bacterial infections. In this advance, herein, we report a liquid crystal (LC) based simple, robust experimental design for rapid and precise recognition of the interaction of LPS with PG and LTA. The optical appearance of nematic 4-cyano-4'-pentylbiphenyl (5CB) LCs changed from dark to bright (consistent with an ordering transition of the LCs) in contact with an aqueous solution of PG and LTA on LPS-laden aqueous-LC interfaces. The ordering transition demonstrates strong interaction between PG and LTA with LPS at these interfaces. Our experiment also revealed that the interaction of PG and LTA towards LPS is highly specific. In addition, PG and LTA shows different binding affinity towards LPS and response of the LC is found to vary significantly from one to another which is conveniently quantified by measurement of the light intensity transmitted through the LC under crossed polars. Langmuir Blodgett (LB) and polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) measurements provide further insight on LPS laden aqueous-LC interfaces. Finally, we have also quantified the different binding affinity of PG and LTA towards LPS by measuring the optical retardance of the LC at aqueous-LC interfaces. Overall, the results presented in this paper offer a promising approach to study and quantify the interactions between different bacterial cell membrane components with LPS at aqueous-LC interfaces.

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“…It can be used as a portable biosensor in resourcelimited regions. Previously, there are many reports of LC-based biosensors for the detection of bacteria (Xu et al, 2010;Zafiu et al, 2016), viruses (Han et al, 2014), enzymes (Hu and Jang, 2012), proteins (Xue and Yang, 2008;Alino et al, 2012;Kim et al, 2018;Kim and Jang, 2019), organophosphate (Yang, 2005), DNA (Yang, 2005;Price and Schwartz, 2008;Chen and Yang, 2010), and glucose (Zhong and Jang, 2014) etc., but it has not been used for the detection of gp-120 due to the lack of specific molecular probes.…”

Section: Introductionmentioning

confidence: 99%

Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein

et al. 2021

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Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions.

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Using liquid crystals for the label-free detection of catalase at aqueous–LC interfaces

Cited by 42 publications

References 31 publications

Chemical and biological sensing using liquid crystals

Chemical and biological sensing using liquid crystals

Design of bio-molecular interfaces using liquid crystals demonstrating endotoxin interactions with bacterial cell wall components

Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein

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