Using Inter Simple Sequence Repeat Multi-Loci Markers for Studying Genetic Diversity in Guppy Fish

Abstract

Aquarium fish industry has become a capacity for job creation and income generation. Aquarium fish uses for decoration and creating tranquility in the environment and beautification in public places. This study was conducted to investigate genetic variation between different populations of various varieties of guppy ornamental aquarium fish using inter simple sequence repeat (ISSR) markers in Iran for the first time. DNA was extracted from twelve strains. Polymerase chain reaction was performed using seven ISSR primers. GenAlex 6.501 and XLSTAT software was used to analyze data. Results of analysis of molecular variance showed that 54% of observed polymorphism belongs to variance among populations and 46% corresponds to diversity between populations. The highest and the lowest polymorphism percentage belonged to (GAG)5GC (90%) and (AG)8C (41%) markers, respectively. Percentage of polymorphic loci ranges from 32.31% to 86.15%. The results of phylogenetic tree showed that this tree was divided into 2 major clusters. Cluster I included only 2 populations and cluster II contained other 10 studied populations. It is suggested that this marker system was found to be efficient in discriminating each genotype at the molecular level and can be used for genetic diversity analysis for aquarium guppy fish because a greater understanding of the potential of the species is necessary for supporting long-term genetic improvement.

GENETIC VARIATION IN TWO PHENOTYPES OF BALI CATTLE (Bos javanicus) INFERRED BY (AG)9C ISSR MARKER

“…In sheep, the (AG)9C ISSR marker has a significant effect on body weight (Zamani et al, 2015;Mohammadabadi, 2016). Thus, this marker has an I value of 0.25 for Mehraban sheep (Zamani et al, 2011) and 1.03 for Kermani sheep (Mohammadabadi et al, 2017). Moreover, the (AG)9C ISSR marker of Tuvinian short-fat-tailed sheep reveals the ne, I, and PIC values of about 1.24; 0.22, and 0.45, respectively (Stolpovsky et al, 2010).…”

KERAGAMAN GENETIK GUPPY (Poecilia reticulata) MENGGUNAKAN METODE RAPD (Random Amplified Polymorphic DNA)

“…Pola warna dikontrol oleh gen dan diturunkan dari induk kepada keturunannya (Laimeheriwa et al, 2019). Guppy merupakan salah satu ikan yang digunakan sebagai model untuk mempelajari genetika populasi, inbreeding, dan heterosis (Mohammadabadi et al, 2021). Penelitian terkait variasi genetik pada beberapa strain ikan Guppy, diantaranya polymorfisme pada guppy jantan (Blows et al, 2003); variasi genetik gen pengontrol pigmen melanin (Tezuka et al, 2011); serta hubungan genetik empat strain Guppy yaitu Albino Full Platinum (AFP), Albino German Yellow (AGY), Top Sword (TS) dan Guppy Yellow Cobra (GYC) menggunakan metode RAPD-PCR (Nuradha et al, 2021).…”

“…Pengetahuan terkait variasi genetik pada ikan Guppy masih sangat diperlukan untuk menghindari terjadinya persilangan antara individu yang memiliki hubungan kekerabatan Jurnal Perikanan, 13 (4), 1032-1042 (2023) http://doi.org/10.29303/jp.v13i3.663 1034 sangat dekat (inbreeding) (Nuradha et al, 2021). Inbreeding dapat menimbulkan konsekuensi negatif seperti menurunnya keragaman genetik (Mohammadabadi et al, 2021). Salah satu teknik yang digunakan untuk mengetahui hubungan kekerabatan tersebut adalah dengan teknik RAPD-PCR.…”

Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics

“…ISSR is a part of the genome between microsatellite sequences in the opposite direction. ISSR is more accurate than RAPD and AFLP because it is stable, effective, efficient (Mohammadabadi et al 2021), and can distinguish genotypes in the population (Daryono et al 2019). This study aimed to determine a maturity stage and predict morphological characters in the melon cultivar 'Melona' as a description of consumer preferences and reveal the molecular character of ISSR to provide information on the stability and uniformity of the Melona cultivar.…”