Using injection molding and reversible bonding for easy fabrication of magnetic cell trapping and sorting devices

Royet, David; Hériveaux, Yoann; Marchalot, Julien; Scorretti, Riccardo; Dias, Ana Carolina; Dempsey, Nora; Bonfim, Marlio; Simonet, Pascal; Frénéa‐Robin, Marie

doi:10.1016/j.jmmm.2016.10.102

Cited by 23 publications

(18 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CI-powder (44890, Sigma–Aldrich) used in this study had a particle size distribution of 5–9 μm, a density of 7.86 g/mL, and a purity of 99.5%, with a color that was pale to dark gray. The magnetic film was prepared by blending a specific ratio of PDMS solution (a 15:1 ratio of Sylgard 184A base to Sylgard 184B curing agent) (Royet et al, 2017). PDMS solution was next added at CI-powder-to-PDMS weight ratios of 2:1, 1:1, 2:1, and 4:1 (CI:PDMS), and was stirred thoroughly for 10 min until uniform.…”

Section: Methodsmentioning

confidence: 99%

The Development of Controllable Magnetic Driven Microphysiological System

Yang

Chen

et al. 2019

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Current research has enabled the use of microphysiological systems and creation of models for alveolar and pulmonary diseases. However, bottlenecks remain in terms of medium- and long-term regulation of cell cultures and their functions in microchannel systems, as well as in the enhancement of in vitro representation of alveolar models and reference values of the data. Currently used systems also require on-chip manufacturing of complex units, such as pumps, tubes, and other cumbersome structures for maintaining cells in culture. In addition, system simplification and minimization of all external and human factors major challenges facing the establishment of in vitro alveolar models. In this study, a magnetically driven dynamic alveolus cell-culture system has been developed to use controlled magnetic force to drive a magnetic film on the chip, thereby directing the fluid within it to produce a circulating flow. The system has been confirmed to be conducive with regard to facilitating uniform attachment of human alveolar epithelial cells and long-term culture. The cell structure has been recapitulated, and differentiation functions have been maintained. Subsequently, reactions between silica nanoparticles and human alveolar epithelial cells have been used to validate the effects and advantages of the proposed dynamic chip-based system compared to a static environment. The innovative concept of use of a magnetic drive has been successfully employed in this study to create a simple and controllable yet dynamic alveolus cell-culture system to realize its functions and advantages with regard to in vitro tissue construction.

show abstract

Section: Methodsmentioning

confidence: 99%

The Development of Controllable Magnetic Driven Microphysiological System

Yang

Chen

et al. 2019

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Also, these DLD example devices possess an enrichment step and use multiple pumps [13] or have been tested to process up to 5 mL samples with a 91% targeted cell capture efficiency [14]. Microfluidic devices that make use of magnetic field gradients to enhance selectivity and increase throughput in cell separation and trapping applications have been developed [10,[15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…As summarized in Table, 1, most of these magnetic separation microfluidic devices used sample volumes, ranging from a few μL to no more than 10 mL [16,17,19,20,[28][29][30][31]. For these volumes, relatively low flow-rates, typically less than 20 µL/s, were sufficient to achieve results in short time [15][16][17][18][28][29][30][31]. For example, Zanini, et al developed a microfluidic device with an integrated array of micromagnets with alternating polarities for the separation of magnetic nanoparticles, which resulted in > 94% particle capture efficiencies (with 0.25 µL/s flow rate) [28].…”

Section: Introductionmentioning

confidence: 99%

A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring

2019

View full text Add to dashboard Cite

The long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as Escherichia coli, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for magnetic separation microfluidic analysis platforms that typically function with <20 µL/s throughput. Here, we report the use of 1.5-µm-diameter magnetic microdisc to selectively tag target bacteria, and a high-throughput microfluidic device that can potentially isolate the magnetically tagged bacteria from 100 mL water samples in less than 15 min. Simulations and experiments show~90% capture efficiencies of magnetic particles at flow rates up to 120 µL/s. Also, the platform enables the magnetic microdiscs/bacteria conjugates to be directly imaged, providing a path for quantitative assay.From the large-volume samples experiments with IONs, the magnetic separation microfluidic device was capable of processing > 50 mL samples (Figure 7). The 50 mL sample (IONs concentration of 100 µg/mL) was filtered in~7 min at a flow rate of 120 µL/s and the estimated capture efficiency was 70.0 ± 2.3%. Similarly, the 100 mL sample (IONs concentration 12.5 µg/mL, 100 mL) was filtered in Micromachines 2020, 11, 16 9 of 13 15 min at a flow rate of 120 µL/s and the estimated capture efficiency was 72.2 ± 2.0%. Figure 7 show 100 mL sample experiment images of the solutions before filtering (A), after filtering (B), the µFMS device with all captured IONs (C), and the VSM data to estimate capture efficiency for 100 mL sample (D).

show abstract

“…If simple and fast fabrication is required, additional PDMS-based magnetic strips in microfluidic devices could lead to enhanced magnetic cell separation [135]. An additional external rotating magnetic field can also be added for further mixing enhancement.…”

Section: Outlook On Magnetic Mixingmentioning

confidence: 99%

Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids

et al. 2019

View full text Add to dashboard Cite

Microfluidic mixing becomes a necessity when thorough sample homogenization is required in small volumes of fluid, such as in lab-on-a-chip devices. For example, efficient mixing is extraordinarily challenging in capillary-filling microfluidic devices and in microchambers with stagnant fluids. To address this issue, specifically designed geometrical features can enhance the effect of diffusion and provide efficient mixing by inducing chaotic fluid flow. This scheme is known as “passive” mixing. In addition, when rapid and global mixing is essential, “active” mixing can be applied by exploiting an external source. In particular, magnetic mixing (where a magnetic field acts to stimulate mixing) shows great potential for high mixing efficiency. This method generally involves magnetic beads and external (or integrated) magnets for the creation of chaotic motion in the device. However, there is still plenty of room for exploiting the potential of magnetic beads for mixing applications. Therefore, this review article focuses on the advantages of magnetic bead mixing along with recommendations on improving mixing in low Reynolds number flows (Re ≤ 1) and in stagnant fluids.

show abstract

Using injection molding and reversible bonding for easy fabrication of magnetic cell trapping and sorting devices

Cited by 23 publications

References 30 publications

The Development of Controllable Magnetic Driven Microphysiological System

The Development of Controllable Magnetic Driven Microphysiological System

A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring

Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids

Contact Info

Product

Resources

About