Using DMSO for chlorophyll spectroscopy

Ritchie, Raymond J.; Sma-Air, Suhailar; Phongphattarawat, Sornsiri

doi:10.1007/s10811-021-02438-8

Cited by 18 publications

(5 citation statements)

References 22 publications

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“…Carotenoids are another group of pigments found in photosynthetic organisms [11] and can be divided in two categories: hydrocarbon carotenes and xanthophylls, which are responsible for creating colours ranging from yellow to red. Although carotenoids are another important pigments in plants, they are masked by chlorophyll and have a minimal influence on plant colour [12]. Carotenoids play a role that protects chlorophyll levels from intensive sunlight.…”

Section: The Effects Of Environmental Stress On Chlorophyll Content 2...mentioning

confidence: 99%

Evaluating the Effects of Environmental Stress on Leaf Chlorophyll Content as an Index for Tree Health

Talebzadeh

Valeo

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Chlorophyll content plays a vital role in photosynthetic and biomass production in all plants. Because chlorophyll shows a greater sensitivity to changes in external conditions than do other pigments in foliage, such as carotenoids for example, chlorophyll content in leaves may be a good surrogate for environmental stress, changes in temperature and humidity, as well as in pollutant levels both in the air and in the soil. This paper reviews the potential for chlorophyll content in the leaves of trees as a measure of tree health, resistance to stress and environmental conditions. Because chlorophyll content is shown to decrease dramatically with increases in pollution, non-destructive methods for evaluating the amount of chlorophyll in leaves and its changes over a time may be a sufficient indicator for environmental pollutant levels in the air, in the soil and in the water used by a tree.

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Section: The Effects Of Environmental Stress On Chlorophyll Content 2...mentioning

confidence: 99%

Evaluating the Effects of Environmental Stress on Leaf Chlorophyll Content as an Index for Tree Health

Talebzadeh

Valeo

2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…By adding DMSO to the supernatants, the volume was increased to 10 mL. The extracts of chlorophyll and carotenoids were placed in a cuvette (volume: 250 μL) and the absorbance was measured at 663, 645, and 470 nm by an Epoch microplate spectrophotometer (USA) (Ritchie et al, 2021).…”

Section: Photosynthetic Pigmentsmentioning

confidence: 99%

Photosynthesis and chlorophyll fluorescence of Iranian licorice (Glycyrrhiza glabra l.) accessions under salinity stress

2022

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While salinity is increasingly becoming a prominent concern in arable farms around the globe, various treatments can be used for the mitigation of salt stress. Here, the effective presence of Azotobacter sp. inoculation (A1) and absence of inoculation (A0) was evaluated on Iranian licorice plants under NaCl stress (0 and 200 mM) (S0 and S1, respectively). In this regard, 16 Iranian licorice (Glycyrrhiza glabra L.) accessions were evaluated for the effects on photosynthesis and chlorophyll fluorescence. Leaf samples were measured for photosynthetic pigments (via a spectrophotometer), stomatal and trichome-related features (via SEM), along with several other morphological and biochemical features. The results revealed an increase in the amount of carotenoids that was caused by bacterial inoculation, which was 28.3% higher than the non-inoculated treatment. Maximum initial fluorescence intensity (F0) (86.7) was observed in the ‘Bardsir’ accession. Meanwhile, the highest variable fluorescence (Fv), maximal fluorescence intensity (Fm), and maximum quantum yield (Fv/Fm) (0.3, 0.4, and 0.8, respectively) were observed in the ‘Eghlid’ accession. Regarding anatomical observations of the leaf structure, salinity reduced stomatal density but increased trichome density. Under the effect of bacterial inoculation, salinity stress was mitigated. With the effect of bacterial inoculation under salinity stress, stomatal length and width increased, compared to the condition of no bacterial inoculation. Minimum malondialdehyde content was observed in ‘Mahabad’ accession (17.8 μmol/g FW). Principle component analysis (PCA) showed that ‘Kashmar’, ‘Sepidan’, ‘Bajgah’, ‘Kermanshah’, and ‘Taft’ accessions were categorized in the same group while being characterized by better performance in the aerial parts of plants. Taken together, the present results generally indicated that selecting the best genotypes, along with exogenous applications of Azotobacter, can improve the outcomes of licorice cultivation for industrial purposes under harsh environments.

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“…where BC 2 and BC 1 represent the biomass concentration on days t 2 and t 1 , respectively. The concentration of photosynthetic pigments was measured by the method of dimethyl sulfoxide (DMSO) extraction [27]. The photosynthetic pigment concentrations were estimated using the following equations:…”

Section: Analysis Of Algal Growthmentioning

confidence: 99%

Phycoremediation Potential of Salt-Tolerant Microalgal Species: Motion, Metabolic Characteristics, and Their Application for Saline–Alkali Soil Improvement in Eco-Farms

Chen,

Yu,

et al. 2024

Microorganisms

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Microalgae have great potential for remediating salt-affected soil. In this study, the microalgae species Coelastrella sp. SDEC-28, Dunaliella salina SDEC-36, and Spirulina subsalsa FACHB-351 were investigated for their potential to rehabilitate salt-affected soils. Nylon screens with optimal aperture sizes and layer numbers were identified to efficiently intercept and harvest biomass, suggesting a correlation between underflow capability and the tough cell walls, strong motility, and intertwining characteristics of the algae. Our investigations proved the feasibility of incorporating monosodium glutamate residue (MSGR) into soil extracts at dilution ratios of 1/200, 1/2000, and 1/500 to serve as the optimal medium for the three microalgae species, respectively. After one growth period of these three species, the electrical conductivities of the media decreased by 0.21, 1.18, and 1.78 mS/cm, respectively, and the pH remained stable at 7.7, 8.6, and 8.4. The hypotheses that microalgae can remediate soil and return profits have been verified through theoretical calculations, demonstrating the potential of employing specific microalgal strains to enhance soil conditions in eco-farms, thereby broadening the range of crops that can be cultivated, including those that are intolerant to saline–alkali environments.

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Using DMSO for chlorophyll spectroscopy

Cited by 18 publications

References 22 publications

Evaluating the Effects of Environmental Stress on Leaf Chlorophyll Content as an Index for Tree Health

Evaluating the Effects of Environmental Stress on Leaf Chlorophyll Content as an Index for Tree Health

Photosynthesis and chlorophyll fluorescence of Iranian licorice (Glycyrrhiza glabra l.) accessions under salinity stress

Phycoremediation Potential of Salt-Tolerant Microalgal Species: Motion, Metabolic Characteristics, and Their Application for Saline–Alkali Soil Improvement in Eco-Farms

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