Using diffuse reflectance spectroscopy probe tracking to identify non-tumour and tumour tissue in upper gastrointestinal specimens

Gkouzionis, Ioannis; Nazarian, Scarlet; Anandakumar, Arun; Darzi, Ara; Patel, Nisha; Peters, Christopher J.; Elson, Daniel S.

doi:10.1117/12.2614476

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Therefore evidence should be available quickly when and where it is needed and be relevant to the task performed [1,2]. In vivo diagnostic imaging methods such as diffuse optical imaging [3,4], diffuse reflectance spectroscopy [5][6][7][8], fluorescence imaging [6,9], optical coherence tomography [10] and in vivo microscopy [11] are being seeing continual development as medical diagnostic techniques. Applications range across medical disciplines, from screening, to tumour margin detection and surgical guidance, and many techniques have been adapted into medical devices seeing real world use.…”

Section: Introductionmentioning

confidence: 99%

Overcoming tissue specular reflection challenges in micro camera endoscopy for in-vivo clinical applications.

Niemitz¹,

Stel²,

Sørensen³

et al. 2023

Preprint

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Surgical guidance and diagnostics by diffuse optical imaging using micro camera technology at the tip endoscopic probes have the potential to act as intra-operative supportive tools for clinicians. Micro camera probes need to address undesirable specular reflections in order to be clinically relevant. In this work we overcome specular reflections caused by the glossy uneven tissue surface. We adapt and compare two techniques for miniaturised probes designed to view tissue. Two camera probes are developed using different modalities to remove these surface reflections, with line-of-sight to further miniaturisation. 1) The multi-flash technique illuminates the sample from four different positions, causing a shift in reflections which is filtered out in a post processing image reconstruction step. 2) The cross polarisation technique integrates orthogonal polarisers on to the tip of the illumination fibres and camera, respectively, to filter out the polarisation maintaining reflections. These form part of an imaging system that is capable of rapid image acquisition using different illumination wavelengths. The system is validated on tissue mimicking phantoms with high surface reflection, as well as excised human breast tissue. It is demonstrated that both methods effectively remove the specular reflections, revealing previously hidden underlying information. The methods demonstrate two effective options for improving image quality in miniaturised systems, for human and machine observers, in a surgical setting.

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Section: Introductionmentioning

confidence: 99%

Overcoming tissue specular reflection challenges in micro camera endoscopy for in-vivo clinical applications.

Niemitz¹,

Stel²,

Sørensen³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, evidence should be rapidly available when and where it is needed and be relevant to the task performed [ 1 , 2 ]. In vivo diagnostic imaging methods such as diffuse optical imaging [ 3 , 4 ], diffuse reflectance spectroscopy [ 5 , 6 , 7 , 8 ], fluorescence imaging [ 6 , 9 ], optical coherence tomography [ 10 ] and in vivo microscopy [ 11 ] are undergoing continual development as medical diagnostic techniques. Applications range across medical disciplines, from screening, to tumour margin detection and surgical guidance, and many techniques have been adapted into medical devices seeing real-world use.…”

Section: Introductionmentioning

confidence: 99%

Microcamera Visualisation System to Overcome Specular Reflections for Tissue Imaging

et al. 2023

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In vivo tissue imaging is an essential tool for medical diagnosis, surgical guidance, and treatment. However, specular reflections caused by glossy tissue surfaces can significantly degrade image quality and hinder the accuracy of imaging systems. In this work, we further the miniaturisation of specular reflection reduction techniques using micro cameras, which have the potential to act as intra-operative supportive tools for clinicians. In order to remove these specular reflections, two small form factor camera probes, handheld at 10 mm footprint and miniaturisable to 2.3 mm, are developed using different modalities, with line-of-sight to further miniaturisation. (1) The sample is illuminated via multi-flash technique from four different positions, causing a shift in reflections which are then filtered out in a post-processing image reconstruction step. (2) The cross-polarisation technique integrates orthogonal polarisers onto the tip of the illumination fibres and camera, respectively, to filter out the polarisation maintaining reflections. These form part of a portable imaging system that is capable of rapid image acquisition using different illumination wavelengths, and employs techniques that lend themselves well to further footprint reduction. We demonstrate the efficacy of the proposed system with validating experiments on tissue-mimicking phantoms with high surface reflection, as well as on excised human breast tissue. We show that both methods can provide clear and detailed images of tissue structures along with the effective removal of distortion or artefacts caused by specular reflections. Our results suggest that the proposed system can improve the image quality of miniature in vivo tissue imaging systems and reveal underlying feature information at depth, for both human and machine observers, leading to better diagnosis and treatment outcomes.

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Using diffuse reflectance spectroscopy probe tracking to identify non-tumour and tumour tissue in upper gastrointestinal specimens

Cited by 2 publications

References 8 publications

Overcoming tissue specular reflection challenges in micro camera endoscopy for in-vivo clinical applications.

Overcoming tissue specular reflection challenges in micro camera endoscopy for in-vivo clinical applications.

Microcamera Visualisation System to Overcome Specular Reflections for Tissue Imaging

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