Using copper ions to amplify ROS-mediated fluorescence for continuous online monitoring of extracellular glucose in living rat brain

Su, Cheng‐Kuan; Chen, Chen-Yu; Tseng, Po‐Jen; Sun, Yuh‐Chang

doi:10.1016/j.bios.2014.09.091

Cited by 6 publications

(2 citation statements)

References 49 publications

(50 reference statements)

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“…The fluorescence determination strategy, following an online enzymatic biochemical derivatization scheme, has received much attention because of its specificity, adaptability, diversity, and cost-effectiveness. , For online in vivo monitoring of rat brain extracellular glucose and lactate, we have, for the first time, immobilized GOx and LOx onto the inner surfaces of 3D-printed ABS flow reactors to convert glucose and lactate, respectively, into H 2 O 2 and related derivatives during the passage of the microdialysate. We applied GA, a cross-linker commonly used to facilitate fixing of biomolecules to electrode surfaces or sensing components, , to link the enzymes to the ABS surface of the 3D-printed object.…”

Section: Resultsmentioning

confidence: 99%

Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

Yen

et al. 2016

Anal. Chem.

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In this study we constructed a highly sensitive system for in vivo monitoring of the concentrations of rat brain extracellular glucose and lactate. This system involved microdialysis (MD) sampling and fluorescence determination in conjunction with a novel sample derivatization scheme in which glucose oxidase and lactate oxidase were immobilized in ABS flow bioreactors (manufactured through low-cost three-dimensional printing (3DP)), via fused deposition modeling, for online oxidization of sampled glucose and lactate, respectively, in rat brain microdialysate. After optimizing the experimental conditions for MD sampling, the manufacture of the designed flow reactors, the enzyme immobilization procedure, and the online derivatization scheme, the available sampling frequency was 15 h(-1) and the system's detection limits reached as low as 0.060 mM for glucose and 0.059 mM for lactate, based on a 20-μL conditioned microdialysate; these characteristics were sufficient to reliably determine the concentrations of extracellular glucose and lactate in the brains of living rats. To demonstrate the system's applicability, we performed (i) spike analyses of offline-collected rat brain microdialysate and (ii) in vivo dynamic monitoring of the extracellular glucose and lactate in living rat brains, in addition to triggering neuronal depolarization by perfusing a high-K(+) medium from the implanted MD probe. Our analytical results and demonstrations confirm that postprinting functionalization of analytical devices manufactured using 3DP technology can be a powerful strategy for extending the diversity and adaptability of currently existing analytical configurations.

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Section: Resultsmentioning

confidence: 99%

Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

Yen

et al. 2016

Anal. Chem.

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“…However, most of the fluorophores or organic molecules used in previous work have broad emission widths and poor photostability. Their fluorescent signals are easily affected by the decay of chromophores and the working environment ( Dong et al, 2013 ; Khan and Pickup, 2013 ; Su et al, 2015 ). To circumvent these issues, kinds of luminescent nanomaterials such as organic-dye doped nanoparticles ( Bagheri et al, 2014 ), semiconductor quantum dots ( Chen et al, 2014 ; Zhai et al, 2016 ; Samuei et al, 2017 ), fluorescent carbon nanodots ( Ma et al, 2017 ; Zou et al, 2018 ), and metal nanoclusters ( Wang et al, 2014 ; Cheng et al, 2018 ) have been devised as the analytical probe because of their preferable photoluminescence properties.…”

Section: Introductionmentioning

confidence: 99%

Glucose determination in human serum by applying inner filter effect quenching mechanism of upconversion nanoparticles

Chen¹,

Yang²,

Chen³

et al. 2023

Front. Bioeng. Biotechnol.

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Accurate blood glucose determination is essential to the clinical diagnosis and management of diabetes. This work establishes an inner filter effect (IFE) strategy between upconversion nanoparticles (UCNPs) and quinone-imine complex for glucose monitoring in human serum simply and efficiently. In this system, the enzyme glucose oxidase (GOx) catalyzes the reaction of glucose into hydrogen peroxide (H2O2) and gluconic acid when compulsion by oxygen. In the presence of horseradish peroxidase (HRP), the produced H2O2 can catalytically oxidize phenol and 4-amino antipyrine (4-AAP) to generate quinone-imine products. The purple-colored quinone-imine complex effectively absorbed the fluorescence of NaYF4:Yb3+, Er3+ UCNPs, leading to the strong fluorescence quenching of UCNPs through IFE. Thus, a new approach was established for glucose monitoring by determining the fluorescence intensity. Under the optimal condition, this approach shows better linearity to glucose from 2–240 μmol/L with a low detection limit at 1.0 μmol/L. Owing to the excellent fluorescence property and background-free interference of the UCNPs, the biosensor was applied for glucose measurements in human serum and got a satisfactory result. Furthermore, this sensitive and selective biosensor revealed great potential for the quantitative analysis of blood glucose or different kinds of H2O2-involved biomolecules for the application of clinical diagnosis.

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3D-printed CuO nanoparticle–functionalized flow reactor enables online fluorometric monitoring of glucose

Tseng

2019

Microchim Acta

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Using copper ions to amplify ROS-mediated fluorescence for continuous online monitoring of extracellular glucose in living rat brain

Cited by 6 publications

References 49 publications

Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

Glucose determination in human serum by applying inner filter effect quenching mechanism of upconversion nanoparticles

3D-printed CuO nanoparticle–functionalized flow reactor enables online fluorometric monitoring of glucose

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