Using anticipatory and drug-evoked appetitive ultrasonic vocalization for monitoring the rewarding effect of amphetamine in a rat model of drug self-administration

Kuchniak, Karolina; Wyszogrodzka, Edyta; Chrapusta, Stanisław J.; Czarna, Magdalena; Michalak, Magdalena; Płaźnik, Adam; Krząścik, Paweł; Mierzejewski, Paweł; Taracha, Ewa

doi:10.1016/j.bbr.2019.112187

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…The studies of Calipari et al [ 54 , 55 ] regarding differences in dopamine signaling in different patterns of cocaine self-administration, suggest that tolerance depends not on the pattern of administration, but on the total cocaine intake within sessions. Similar patterns of behavior were demonstrated in our previous study regarding amphetamine self-administration [ 56 ].…”

Section: Discussionsupporting

confidence: 87%

Molecular pattern of a decrease in the rewarding effect of cocaine after an escalating-dose drug regimen

et al. 2022

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Background Long-term cocaine exposure leads to dysregulation of the reward system and initiates processes that ultimately weaken its rewarding effects. Here, we studied the influence of an escalating-dose cocaine regimen on drug-associated appetitive behavior after a withdrawal period, along with corresponding molecular changes in plasma and the prefrontal cortex (PFC). Methods We applied a 5 day escalating-dose cocaine regimen in rats. We assessed anxiety-like behavior at the beginning of the withdrawal period in the elevated plus maze (EPM) test. The reinforcement properties of cocaine were evaluated in the Conditioned Place Preference (CPP) test along with ultrasonic vocalization (USV) in the appetitive range in a drug-associated context. We assessed corticosterone, proopiomelanocortin (POMC), β-endorphin, CART 55–102 levels in plasma (by ELISA), along with mRNA levels for D2 dopaminergic receptor (D2R), κ-receptor (KOR), orexin 1 receptor (OX1R), CART 55–102, and potential markers of cocaine abuse: miRNA-124 and miRNA-137 levels in the PFC (by PCR). Results Rats subjected to the escalating-dose cocaine binge regimen spent less time in the cocaine-paired compartment, and presented a lower number of appetitive USV episodes. These changes were accompanied by a decrease in corticosterone and CART levels, an increase in POMC and β-endorphin levels in plasma, and an increase in the mRNA for D2R and miRNA-124 levels, but a decrease in the mRNA levels for KOR, OX1R, and CART 55–102 in the PFC. Conclusions The presented data reflect a part of a bigger picture of a multilevel interplay between neurotransmitter systems and neuromodulators underlying processes associated with cocaine abuse.

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Section: Discussionsupporting

confidence: 87%

Molecular pattern of a decrease in the rewarding effect of cocaine after an escalating-dose drug regimen

et al. 2022

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“…The rats emit 50-kHz USV during different elements of behavior related to their high arousal states ( Bell, 1974 ; Berz et al, 2021 ) such as: socio-sexual interactions including copulation ( Barfield et al, 1979 ; Bialy et al, 2000 ; Burgdorf et al, 2008 ), fighting ( Sales, 1972 ; Burke et al, 2017 ), playing ( Reinhold et al, 2019 ), and even tickling by the experimenter ( Burgdorf et al, 2005 ; Panksepp, 2005 ). Additionally, pharmacologically induced high levels of general arousal [related to movement activity and sensory sensitization associated with increased level of wakefulness during activation of the gigantocellular reticular nucleus and associated structures ( Pfaff, 2017 )] result in the expression of 50-kHz vocalizations with a strong positive correlation between the number of 50-kHz ultrasounds emitted and the level of activation of the dopaminergic and noradrenergic pathways ( Brudzynski, 2007 , 2015 ; Wright et al, 2012 ; Simola, 2015 ; Hamed et al, 2016 ; Simola and Costa, 2018 ; Kuchniak et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Anticipatory 50-kHz Precontact Ultrasonic Vocalizations and Sexual Motivation: Characteristic Pattern of Ultrasound Subtypes in an Individual Analyzed Profile

Bogacki-Rychlik

Rolf

Bialy

2021

Front. Behav. Neurosci.

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We verified the hypothesis of the existence of forms of individual-specific differences in the emission of anticipatory precontact vocalization (PVs) indicating individualization related to sexual experience and motivation in male rats. Long-Evans males were individually placed in a chamber and 50-kHz ultrasounds were recorded during 5-min periods. In experiment 1, PVs were recorded before the introduction of a female in four consecutive sessions during the acquisition of sexual experience. In experiment 2, PVs were analyzed in three groups of sexually experienced males: with the highest, moderate, and the lowest sexual motivation based on previous copulatory activity. In both experiments, the total number of ultrasounds, as well as 14 different specific subtypes, was measured. The ultrasound profiles for each male were created by analyzing the proportions of specific dominant subtypes of so-called 50-kHz calls. We decided that the dominant ultrasounds were those that represented more than 10% of the total recorded signals in a particular session. The number of PVs was positively correlated with the acquisition of sexual experience and previous copulatory efficiency (measured as the number of sessions with ejaculation). Furthermore, PVs showed domination of the frequency modulated signals (complex and composite) as well as flat and short with upward ramp ultrasounds with some individual differences, regardless of the level of sexual motivation. The results show a characteristic pattern of PVs and confirm the hypothesis that the number of PVs is a parameter reflecting the level of sexual motivation.

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“…That increase in negative calls plus the lack of major changes in the call rates of either frequency at ABS highlight the presence of negative affect during drug use after repeated exposure to the drug, not just once the drug has left the body and withdrawal sets in. The mix of both 22 and 50 kHz calls during the 6 h ABS session may reflect fluctuations between a negative affective state associated with opioid withdrawal [6] and a positive affective state associated with anticipation of drug for the 31st consecutive day, since 50 kHz calls can indicate an anticipatory state [8,33,34].…”

Section: Discussionmentioning

confidence: 99%

Chronic Fentanyl Self-Administration Generates a Shift toward Negative Affect in Rats during Drug Use

et al. 2021

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Drug addiction is thought to be driven by negative reinforcement, and it is thought that a shift from positive affect upon initial exposure to negative affect after chronic exposure to a drug is responsible for maintaining self-administration (SA) in addicted individuals. This can be modeled in rats by analyzing ultrasonic vocalizations (USVs), a type of intraspecies communication indicative of affective state based on the frequency of the emission: calls in the 22 kHz range indicate negative affect, whereas calls in the 50 kHz range indicate positive affect. We employed a voluntary chronic, long-access model of fentanyl SA to analyze affective changes in the response to chronic fentanyl exposure. Male Sprague-Dawley rats self-administered either fentanyl (N = 7) or saline (N = 6) for 30 consecutive days and USVs were recorded at four different time points: the day before first SA session (PRE), the first day of SA (T01), the last day of SA (T30), and the first day of abstinence (ABS). At T01, the ratio of 50 to 22 kHz calls was similar between the fentanyl and saline groups, but at T30, the ratio differed between groups, with the fentanyl group showing significantly fewer 50 kHz calls and more 22 kHz calls relative to saline animals. These results indicate a shift toward a negative affect during drug use after chronic exposure to fentanyl and support negative reinforcement as a main driving factor of opioid addiction.

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Using anticipatory and drug-evoked appetitive ultrasonic vocalization for monitoring the rewarding effect of amphetamine in a rat model of drug self-administration

Cited by 11 publications

References 48 publications

Molecular pattern of a decrease in the rewarding effect of cocaine after an escalating-dose drug regimen

Molecular pattern of a decrease in the rewarding effect of cocaine after an escalating-dose drug regimen

Anticipatory 50-kHz Precontact Ultrasonic Vocalizations and Sexual Motivation: Characteristic Pattern of Ultrasound Subtypes in an Individual Analyzed Profile

Chronic Fentanyl Self-Administration Generates a Shift toward Negative Affect in Rats during Drug Use

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