Using an Electrical Potential to Reversibly Switch Surfaces between Two States for Dynamically Controlling Cell Adhesion

Ng, Cheuk Chi Albert; Magenau, Astrid; Ngalim, Siti Hawa; Ciampi, Simone; Chockalingham, Muthukumar; Harper, Jason B.; Gaus, Katharina; Gooding, J. Justin

doi:10.1002/ange.201202118

Cited by 41 publications

(38 citation statements)

References 29 publications

(18 reference statements)

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“…It is easy to operate, fast, highly efficient, and also can be widely employed on different surfaces [20]. To efficiently control cell behavior, previous electrochemical approaches often involve to controlled reductive desorption of the Au-S bonds on surface by the application of a negative voltage, triggered electrochemically using of the hydroquinone/ quinone redox pair or switched a surface from hydrophilic to hydrophobic, etc., which then usually mediated the surface binding peptide sequence Arg-Gly-Asp (RGD) as recognition probe for cell adhesion [15,[21][22][23][24]. However, due to the longtime applied potential on surface [15] and irreversible formation or breaking of chemical bonds [21][22][23][24], an ultrasensitive electrochemical substrate for cell switching is highly required.…”

Section: Introductionmentioning

confidence: 99%

“…To realize such control, different functional substrates have been developed, where cell adhesion can be controlled in response to external stimuli, such as heat [9,10], chemicals [11], light [12][13][14], potential and enzymatic activities [15,16], etc. However, most of the stimuli-responsive substrate focuses on the single response till now, which would not satisfy the needs of double or multiple stimulation in future applications.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Feng

Ren

et al. 2014

Nano Res.

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Here we report a dual-functionalized electrochemical substrate to trigger cancer cells release based on the supramolecular interaction between β-cyclodextrin (β-CD) and Fc on clinical trial II aptamer AS1411 functionalized graphene platform. On one hand, the host-guest interaction can be reversible electrochemically controlled to realize cancer cells capture/release, and 1-adamantylamine binding can further amplify this surface change by competing interaction with β-CD. On the other hand, the AS1411 aptamer and its complementary DNA (cDNA) also can be used as a switchable anchor for cell adhesion. Our work gives an example for label-free, multi-functionalized triggered cell release based on aptamer and β-CD/graphene-modified surface and this multi-ways for cell catch-and-release on graphene modified surface also provides their potential biomedical application.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Feng

Ren

et al. 2014

Nano Res.

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“…These switchable surfaces are playing an increasingly important part in the development of highly sensitive biosensors [85], novel drug delivery systems [148] and highly functional microfluidic [277], bioanalysis [278], and bioseparation [279] systems. Additionally, dynamic, synthetic surfaces that can control the presentation of regulatory signals [220,280] to a cell are expected to have a significant impact in tissue engineering [176] and regenerative medicine [208], and to provide unprecedented opportunities in fundamental studies of cell biology.…”

Section: Resultsmentioning

confidence: 99%

“…SPR has further shown that these responsive surfaces can control binding ability to greater than 90%. Following this work, Gooding and coworkers [220] have extended the concept of molecular mechanical motions of surface-bound electro-switchable molecules to control cell adhesion. The two-component SAMs comprised a protein-resistant hexa(ethylene glycol) (EG6) chain, which contained a charged moiety on its distal end, and a terminal RGD component on which cellular adhesion receptors, integrins, can bind (Fig.…”

Section: Samsmentioning

confidence: 99%

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Stimuli‐Responsive Surfaces in Biomedical Applications

Pranzetti¹,

Preece²,

Mendes³

2013

Intelligent Stimuli‐Responsive Materials

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Nature provides mechanisms that are able to dynamically control specific and nonspecific interactions between cells and biological surfaces [1,2]. Scientists have long tried to reproduce these dynamic biological events and have recently made an important step in that direction by creating artificial stimuli-responsive surfaces [3][4][5][6][7]. These smart substrates present modulatory surface properties that are able to respond to external chemical/biochemical [8][9][10][11][12], thermal [13][14][15], electrical [16][17][18][19][20], and optical stimuli [21][22][23][24][25][26][27][28][29][30][31]. Due to their dynamic nature such substrates are very appealing for applications in the biomedical field [32]. Progress to date has led to control over biomolecule activity [33] and immobilization of a diverse array of proteins, including enzymes [34] and antibodies [35]. These prior achievements have encouraged researchers to take the challenge of using dynamic surfaces to modulate larger and more complex systems, such as bacteria [36] and mammalian cells [37].Achieving control over surface properties could provide new insights in the understanding of cell behavior and can offer distinct benefits with regard to the development of medical devices. For instance, the modulation of cell attachment and detachment could lead to the prevention of unwanted bacteria fouling on implants, reducing the risk of infections and rejection [38][39][40][41][42]. Furthermore, dynamic surfaces able to present on demand regulatory signals to a cell could provide unprecedented opportunities in studies of cell responses in real-time. Cells in tissues adhere to and interact with their extracellular environment via specialized cell-cell and cell-extracellular

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About Chemical Strategies to Fabricate Cell‐Instructive Biointerfaces with Static and Dynamic Complexity

Koçer

Jonkheijm

2018

Adv Healthcare Materials

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Properly functioning cell-instructive biointerfaces are critical for healthy integration of biomedical devices in the body and serve as decisive tools for the advancement of our understanding of fundamental cell biological phenomena. Studies are reviewed that use covalent chemistries to fabricate cell-instructive biointerfaces. These types of biointerfaces typically result in a static presentation of predefined cell-instructive cues. Chemically defined, but dynamic cell-instructive biointerfaces introduce spatiotemporal control over cell-instructive cues and present another type of biointerface, which promises a more biomimetic way to guide cell behavior. Therefore, strategies that offer control over the lateral sorting of ligands, the availability and molecular structure of bioactive ligands, and strategies that offer the ability to induce physical, chemical and mechanical changes in situ are reviewed. Specific attention is paid to state-of-the-art studies on dynamic, cell-instructive 3D materials. Future work is expected to further deepen our understanding of molecular and cellular biological processes investigating cell-type specific responses and the translational steps toward targeted in vivo applications.

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Using an Electrical Potential to Reversibly Switch Surfaces between Two States for Dynamically Controlling Cell Adhesion

Cited by 41 publications

References 29 publications

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Stimuli‐Responsive Surfaces in Biomedical Applications

About Chemical Strategies to Fabricate Cell‐Instructive Biointerfaces with Static and Dynamic Complexity

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