Usefulness of the lymphocyte positional parameters in the Sysmex <scp>XN</scp> haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Furundarena, Jose Ramon; Uranga, Alasne; Sainz, Marta; González, Carmen González; Uresandi, N.; Argoitia, N.; Araiz, Maria

doi:10.1111/ijlh.12726

Cited by 13 publications

(16 citation statements)

References 10 publications

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“…These represent numerical values correlated to the intrinsic biophysical properties and morphology of leukocytes. UniCel DxH 800 haematology analyser measures positional parameter with VCS technology (Volume, Conductivity, and Scatter), using 3 independent energy sources simultaneously: direct current impedance to measure cell volume of all cell types; radio frequency opacity, to characterize conductivity for internal composition of each cell; a laser beam to measure light scatter for cytoplasmic granularity and nuclear structure [ 20 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Monocyte distribution width (MDW) as a new tool for the prediction of sepsis in critically ill patients: a preliminary investigation in an intensive care unit

et al. 2021

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Background Monocyte Distribution Width (MDW), a simple proxy marker of innate monocyte activation, can be used for the early recognition of sepsis along with Procalcitonin. This study explored the added value of MDW as an early predictor of ensuing sepsis in patients hospitalised in an Intensive Care Unit. Methods We performed an observational prospective monocentric study to estimate the analytical performance of MDW in detecting ensuing sepsis in a sample of consecutive patients assisted in an Intensive Care Unit for > 48 h for any reason. Demographic and clinical characteristics, past medical history and other laboratory measurements were included as potential predictors of confirmed sepsis in multivariate logistic regression. Results A total of 211 patients were observed, 129 of whom were included in the final sample due to the suspect of ensuing sepsis; of these, 74 (57%) had a confirmed diagnosis of sepsis, which was best predicted with the combination of MDW > 23.0 and PCT > 0.5 ng/mL (Positive Predictive Value, PPV: 92.6, 95% CI: 82.1–97.9). The best MDW cut-off to rule out sepsis was ≤20.0 (Negative Predictive Value, NPV: 86.4, 95% CI: 65.1–97.1). Multivariate analyses using both MDW and PCT found a significant association for MDW > 23 only (OR:17.64, 95% CI: 5.53–67.91). Conclusion We found that values of MDW > 23 were associated with a high PPV for sepsis, whereas values of MDW ≤ 20 were associated with a high NPV. Our findings suggest that MDW may help clinicians to monitor ICU patients at risk of sepsis, with minimal additional efforts over standard of care.

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Section: Methodsmentioning

confidence: 99%

Monocyte distribution width (MDW) as a new tool for the prediction of sepsis in critically ill patients: a preliminary investigation in an intensive care unit

et al. 2021

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“…Since most individual CBC or VCS parameters were not powerfully discriminatory between the two groups, combinatorial approaches were used to construct LIS-compatible logistic regression equations/models with binary outcomes i.e. 0 or 1 corresponding to benign or clonal etiologies [12]. Different strategies were adopted to obtain these models-forward, backward and manual stepwise regression procedures.…”

Section: Discussionmentioning

confidence: 99%

“…overfitting), we focused on the percentage of correctly classified cases rather than ROC curves in selecting best models. The models' optimal cut-offs were derived using receiver operating characteristics (ROC) curves [12].…”

Section: Discussionmentioning

confidence: 99%

Leukocyte Cell Population Data for Hematology Analyzer-Based Distinction of Clonal-versus-Non-Clonal Lymphocytosis: A Real-World Testing Experience

Rastogi¹,

Sharma²,

Varma³

et al. 2018

Indian J Hematol Blood Transfus

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“…Recent studies showed that NE CPD parameters (i.e., NE-SSC, NE-SFL) may be useful for diagnosing myelodysplastic syndromes and sepsis, whilst NE-SFL and MO-WX may play a role in differential diagnosis of some acute leukemias (4, 5, 6, 7, 8, 9). Some LY CPD parameters (i.e., LY-X, LY-Y and LY-Z) may then provide valuable information for screening lymphoproliferative diseases (10, 11). Nevertheless, little information is available on the analytical quality specification of CPD, and even less is known on the degree of harmonization of these measures among different instrumentation and clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Proposal for Quality Control Assessment and Harmonization of Leukocytes Morphology-Structural Parameters (Cell Population Data Parameters)

Seghezzi¹,

Buoro²,

Previtali³

et al. 2018

Journal of Medical Biochemistry

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SummaryBackgroundThe cell population data (CPD) measured by Sysmex XN-9000 can be used for screening many hematological and non-hematological disorders. Since little information is available on harmonization of CPD among different instrumentation and clinical laboratories, this study aimed at assessing the current degree of CPD harmonization between separate Sysmex XN modules allocated to the same laboratory.MethodsA total number of 78291 data were used for verification of within-run imprecision, analyzers harmonization, reference ranges and assessment of blood sample stability of CPD parameters, including results of daily quality control testing and those generated in samples collected from blood donors and healthy volunteers.ResultsWithin-run imprecision of CPD parameters ranged between 0.4 and 14.1%. Good agreement was found among five different XN-modules, especially when values were adjusted after calculation of instrument-specific alignment factors. The bias of all parameters remained always lower than the reference change values in samples stored for up to 8 hours, regardless of storage temperature.ConclusionsThe imprecision of CPD parameters was acceptable, except for those reflecting the dispersion of cellular clusters. Due to the lack of reference control materials, we showed that the use of data generated on a large number of normal routine samples (i.e., a Moving Average population) may be a reliable approach for testing analyzers harmonization. Nevertheless, availability of both calibration and quality control materials for these parameters is highly advisable in the future. We finally showed that whole blood samples may be stable for up to 2–4 hours for most CPD parameters.

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Usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Cited by 13 publications

References 10 publications

Monocyte distribution width (MDW) as a new tool for the prediction of sepsis in critically ill patients: a preliminary investigation in an intensive care unit

Monocyte distribution width (MDW) as a new tool for the prediction of sepsis in critically ill patients: a preliminary investigation in an intensive care unit

Leukocyte Cell Population Data for Hematology Analyzer-Based Distinction of Clonal-versus-Non-Clonal Lymphocytosis: A Real-World Testing Experience

A Preliminary Proposal for Quality Control Assessment and Harmonization of Leukocytes Morphology-Structural Parameters (Cell Population Data Parameters)

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