A Preliminary Proposal for Quality Control Assessment and Harmonization of Leukocytes Morphology-Structural Parameters (Cell Population Data Parameters)

Seghezzi, Michela; Buoro, Sabrina; Previtali, Giulia; Moioli, Valentina; Manenti, Barbara; Simon-Lopez, Ramon; Ottomano, Cosimo; Lippi, Giuseppe

doi:10.2478/jomb-2018-0005

Cited by 11 publications

(8 citation statements)

References 21 publications

(27 reference statements)

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“…9,10 Here we considered the clinical utility of CPD parameters, LY-Y parameter showed a particular trend in our study with negative correlation in sepsis cases, which was consistent with Buoro et al However, the major disadvantage of these CPD parameters were less analytical quality specification and harmonization among different instruments and clinical laboratories. 11 This was rectified to an extent by the study done on quality control assessment and harmonization of CPD parameters by Seghezzi et al They found minimal imprecision for all CPD parameters except for those reflecting width of dispersion .Though in our study, no imprecision test was performed, combining our statistical analysis with Seghezzi et al we would like to streamline our findings to all CPD parameters with exception NE-SFC, MO-Z, LY-Y and those reflecting width of dispersion would aid in early diagnosis of sepsis.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Utility of Cell Population Data (CPD) In Sepsis Using Automated Hematology Analysers

Rao

et al. 2019

APALM

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Background: Sepsis still remains an important cause of morbidity and mortality posing a diagnostic challenge to clinicians across the globe. Lack of a single specific and sensitive test paved the way to many researches. Our study evaluated clinical significance of cell population data (CPD) as biomarkers in sepsis. CPD assesses morphological and functional characteristics of leucocytes using automated hematology analyzer. Methods:The study population consisted of 100 healthy subjects and 100 clinically suspected cases of sepsis with quick sequential organ failure assessment (qSOFA) score >2. The various cell population data (CPD) were collected using automated hematology analyzer, Sysmex XN1000 during a span of 6 months from January to June 2018 in a tertiary care center and the results were statistically analyzed using Z test. Result:The WBC count and CPD parameters were assessed. Except for neutrophil , monocyte cell size (NE-FSC, MO-Z) and lymphocyte fluorescence intensity(LY-Y) all other CPD parameters show significant difference (p<0.001) in sepsis group compared to healthy controls. Conclusion:This study suggested utility of CPD parameters which provide details of size and internal structure of leucocytes to be considered as an important biomarker for diagnosis and management of sepsis.

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Section: Discussionmentioning

confidence: 99%

Diagnostic Utility of Cell Population Data (CPD) In Sepsis Using Automated Hematology Analysers

Rao

et al. 2019

APALM

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“…Along the lack of harmonization among different instruments and clinical laboratories, another disadvantage of these CPD parameters is less analytical quality specification. Metrological issues, such as stability, quality control targets and harmonization of CPD, are now under intense evaluation (43,44).…”

Section: Cpd: Opportunity and Weaknessmentioning

confidence: 99%

Reviewing the value of leukocytes cell population data (CPD) in the management of sepsis

Urrechaga¹

2020

Ann Transl Med

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Sepsis is a medical emergency that describes the body's systemic immune response to an infection and can lead to end-stage organic dysfunction and death. Despite the advances in understanding the pathophysiology of this syndrome and therapies, sepsis remains one of the leading causes of morbidity and mortality in critically ill patients. Early diagnosis and rapid intervention are essential to improve outcomes, which inspired the concept "golden hour," during which the correction of shock and organic dysfunction can improve the patients' outcomes. But the initial presentation of sepsis is often nonspecific and its severity is difficult to assess. Anomalies in temperature, heart and respiratory rates and leukocyte counts are manifestations of systemic inflammatory response syndrome (SIRS). Diagnosis, management and followup of patients with sepsis remains a challenge, and diverse biomarkers have been proposed for the timely diagnosis and prognosis of septic patients: lactic acid, procalcitonin (PCT), C-reactive protein, immature granulocytes. The host's initial response to infection is a humoral, cellular and neuroendocrine reaction to infection, and leukocytes interact with endothelial cells. The new generation of hematological analyzers incorporates technological innovations allowing to expand the information derived from the complete blood count: new leukocyte derived parameters are emerging as potentially useful markers in different clinical situations. Additional research parameters cell population data (CPD), characterizing different leukocyte populations have become available, and preliminary observations suggest their utility in the diagnosis of sepsis. This review emphasizes the value of CPD, reported by modern cellular counters for early recognition of sepsis, and therefore the potential improvement in patient outcomes.

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“…Most of the studies performed in this field so far have been carried out using the hematological analyzer of the Sysmex® laboratory, since it is one of the few that incorporate CPD parameters. 15,18 These parameters prove to be interesting in several aspects as biomarkers, in addition to providing information on changes in leukocytes in a quantitative way, they are obtained very quickly and more accurately during automated differential analysis without additional sample requirements, in an objective and cost-effective manner, They are considered very advantageous when working and analyzing in the laboratory, being useful for the management of hematological pathology, neoplasm and infection, being postulated as predictive and novel parameters in the detection of infection and sepsis. 20 Regarding the results obtained from CPD in this study, they are in agreement with some published studies 2,9,10,12 where of the CPD parameters studied, they suggest that MON-X and NEU-Y parameters may provide useful information in the management of septic patients and be of CPD the best candidates as biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…12,13,15,16 Most of the studies performed in this field to date have been carried out using the hematological analyzer of the Sysmex® laboratory since it is one of the few that incorporate CPD parameters. [17][18][19][20] This is one of the first studies to utilize the BC 6800 Plus Mindray® analyzer to obtain CPD parameters data for neutrophils (NEU), lymphocytes (LYM) and monocytes (MON). The emitted optical signals indicate changes in internal cellular complexity (NEU-X, LYM-X and MON-X), nucleic acid content (NEU-Y, LYM-Y, MON-Y) and cell size (NEU-Z, LYM-Z, MON-Z).…”

Section: Introductionmentioning

confidence: 99%

Analysis of leukocyte cell population data (CPD) as biomarkers in the diagnosis of sepsis

IM¹,

R²,

Bellido³

et al. 2022

HTIJ

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Background: Leukocyte cell population data (CPD) are parameters currently being studied as biomarkers in sepsis and other pathologies. CPD was procured with the BC 6800 Plus Mindray® hematology analyzer that also provides information on the internal cellular complexity, nucleic acid content, and size of leukocytes. This study aimed to assess the efficacy of CPD parameters as biomarkers in the diagnosis of sepsis by comparing these with the standard sepsis biomarker, procalcitonin (PCT). Methods: In total 107 patients with suspected sepsis were included in the study, and serum procalcitonin levels were measured and WBC cell population data (CPD) were analyzed in a hemogram to confirm the diagnostic accuracy of these biomarkers. ROC curves were plotted for all parameters and patients were categorized based on their serum procalcitonin levels. IBM SPSS Stadistics version 24 was used for statistical analysis of the data. Results: All parameters except the CPD NEU-X (neutrophil complexity), NEU-Z (neutrophil size), LYM-Z (lymphocyte size) and MON-Z (monocyte size) showed statistically significant results (p<0.05). ROC curve analysis showed that the CPD parameters MON-X and NEU-Y had area under the ROC curve (AUC) > 0.7, reflecting the better diagnostic performance in sepsis. A lower AUC of 0.650 was obtained for PCT; 87.8% of septic patients had serum PCT levels between ≥5 ng/mL and 51.2% ≥ 2 ng/mL. Conclusion: This study suggests that some of the new CPD parameters (MON-X and NEU-Y) have the potential to be a useful diagnostic marker for sepsis.

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A Preliminary Proposal for Quality Control Assessment and Harmonization of Leukocytes Morphology-Structural Parameters (Cell Population Data Parameters)

Cited by 11 publications

References 21 publications

Diagnostic Utility of Cell Population Data (CPD) In Sepsis Using Automated Hematology Analysers

Diagnostic Utility of Cell Population Data (CPD) In Sepsis Using Automated Hematology Analysers

Reviewing the value of leukocytes cell population data (CPD) in the management of sepsis

Analysis of leukocyte cell population data (CPD) as biomarkers in the diagnosis of sepsis

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